Product Details

Uniprot No.

Target Names

Alternative Names

B-cell lymphoma/leukemia 10 (B-cell CLL/lymphoma 10) (Bcl-10) (CARD-containing molecule enhancing NF-kappa-B) (CARD-like apoptotic protein) (hCLAP) (CED-3/ICH-1 prodomain homologous E10-like regulator) (CIPER) (Cellular homolog of vCARMEN) (cCARMEN) (Cellular-E10) (c-E10) (Mammalian CARD-containing adapter molecule E10) (mE10), BCL10, CIPER CLAP

Species Reactivity

Human

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4D9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BCL10 serves as a critical signaling adapter protein that bridges antigen receptor activation to NF-κB pathway engagement, making it essential for understanding lymphocyte development, immune responses, and lymphomagenesis. Originally identified through its involvement in the t(1;14) translocation associated with MALT lymphoma, BCL10 has since emerged as a key player in the CBM signalosome complex, where it facilitates signal transduction from diverse immunoreceptors.

This recombinant monoclonal antibody, clone 4D9, offers the reproducibility and consistency that demanding research applications require. Generated in rabbit and produced through recombinant technology, it provides sequence-defined specificity against human BCL10, eliminating the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human BCL10, ensuring targeted epitope recognition.

Validation studies demonstrate reliable performance across multiple platforms. In western blot analysis using A431 whole cell lysate, the antibody detects a band at approximately 31 kDa, slightly larger than the predicted 26 kDa molecular weight. This size difference likely reflects post-translational modifications, including phosphorylation events known to regulate BCL10 function during immune signaling. Flow cytometry validation in BxPC-3 cells confirms robust intracellular detection with clear separation from isotype control, supporting applications in cell-based signaling studies.

With validated performance in ELISA and flow cytometry at working dilutions of 1:50–1:200, this antibody supports diverse experimental workflows investigating NF-κB signaling, lymphocyte biology, and oncogenic mechanisms. Researchers studying MALT lymphoma pathogenesis, T-cell receptor signaling, or inflammatory pathway regulation will find this reagent well-suited to their investigative needs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation. Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex. This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity. Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK.

Gene References into Functions

Results show that GSK3beta modulates serine phosphorylation of BCL10. PMID: 29358699
BCL10 forms CARMA1-BCL10-MALT1-TRAF6 signalosome and BCL10 polymerizes in a unidirectional manner. PMID: 29382759
The results suggest that the involvement of BCL10 in DNA damage-induced NF-kappaB is through the recruitment of TRAF6. PMID: 28717989
results define molecular determinants that control the production of Lin(Ub)n-Bcl10, an important signaling intermediate in TCR and oncogenic CARD11 signaling. PMID: 27777308
Psoriasis mutations disrupt CARD14 autoinhibition promoting BCL10-MALT1-dependent NF-kappaB activation PMID: 27071417
BCL10 promoted DNA double-strand breaks repair, enhancing cell survival after DNA damage. PMID: 26771713
Data show that caspase recruitment domain-containing protein 11/B-cell CLL/lymphoma 10/mucosa-associated lymphoid tissue lymphoma translocation gene 1 signaling drives lymphoproliferation through NF-kappa B and c-Jun N-terminal kinase activation. PMID: 26668357
BCL10 is not essential for actin polymerization after fibroblast FcgammaR stimulation. PMID: 26774590
characterization of zebrafish (Danio rerio) Bcl10 PMID: 25849213
we identified BCL10 as a bona fide target of BCR-induced linear ubiquitylation and demonstrated an important role of the linear ubiquitin ligase HOIP in BCR-induced phosphorylation PMID: 26038114
The results of this study indicate that inherited BCL10 deficiency should be considered in patients with combined immunodeficiency with B cell, T cell, and fibroblast defects. PMID: 25365219
B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
BCL10 induces cleavage of MALT1 at R149 in 293T cells. PMID: 25105596
Overexpression of CARMA-BCL10-MALT in T-ALL may contribute to the constitutive cleavage and inactivation of A20, which enhances NF-kappaB signaling and may be related to T-ALL pathogenesis. PMID: 25384343
BCL10 delivers UBC13 to RNF8/RNF168 to regulate ubiquitination-mediated double-strand break signaling and repair. PMID: 24732096
elevated in cholesteatoma PMID: 24702227
A novel susceptibility locus on 1p22 was discovered, which implicates BCL10 as a new susceptibility gene for leprosy. PMID: 23784377
Combining crystallography, nuclear magnetic resonance, and electron microscopy, we reveal the structure of the Bcl10 CARD filament and the mode of interaction between CARMA1 and Bcl10 PMID: 24074955
BCL10 expression is a useful marker for acinar cell differentiation, particularly in the diagnosis of endoscopic ultrasound-guided fine-needle aspiration specimens of Acinar cell carcinomas of the pancreas PMID: 23530562
Bcl10 links saturated fat overnutrition with hepatocellular NF-kB activation and insulin resistance. PMID: 22708078
BCL10 was commonly down-regulated in peripheral T cell lymphomas, suggest the T-cell receptor signaling cascade for future characterization. PMID: 22818167
identify Bcl10 as an early coordinator of NF-kappaB-mediated immune response with endosomal trafficking and signaling to F-actin remodeling PMID: 23153494
Results indicate that NF-kappaB binding to the BCL10 promoter can lead to prolonged activation of the carrageenan-induced inflammatory cascade by a transcriptional mechanism involving an NF-kappaB-BCL10 loop. PMID: 22579587
A proapoptotic role for protein kinase C zeta in the binding and phosphorylating Bcl10 at the nuclear envelope. PMID: 22812606
BCL10 plays an important role in controlling the growth of cervical cancer cells through NF-kappaB dependent cyclin D1 regulation. PMID: 22564715
FOXO3a promotes cell survival via BCL10/NF-kappaB in serum starvation PMID: 22474286
identify MIB2 as a novel component of the activated BCL10 signaling complex and a missing link in the BCL10-dependent NF-kappaB signaling pathway. PMID: 21896478
findings indicate that BCL10 phosphorylations act upstream of phosphorylations of NIK, TAK1, and IkappaBalpha and differentially affect the canonical and noncanonical pathways of NF-kappaB activation PMID: 21700900
Findings demonstrate that CaN functions as a critical signaling molecule during Th cell activation, regulating Bcl-10 phosphorylation and NF-kappaB activation. PMID: 21674474
study shows CaMKII is recruited to the immunological synapse where it interacts with and phosphorylates Bcl10; propose a mechanism whereby Ca(2+) signals can be integrated at the immunological synapse through CaMKII-dependent phosphorylation of Bcl10 PMID: 21513986
Data show that CARMA3 and Bcl10 contributed to several characteristics of EGFR-associated malignancy, including proliferation, survival, migration, and invasion. PMID: 21406399
Lipopolysaccharide induces activation of both canonical and non-canonical pathways of NF-kappaB and the non-canonical pathway requires phosphorylations of BCL10 (serine 138) and NIK. PMID: 20466000
A novel mutation of Bc1-10 gene in ocular adnexal MALT lymphoma was detected in Chinese patients. PMID: 18307945
BCL10 nuclear expression is common in ocular adnexal mucosa-associated lymphoid tissue lymphomas. PMID: 19035248
activation of NF-kappaB by CXCR4 occurs through Carma3/Bcl10/Malt1 (CBM) complex in OSCC. loss of components of CBM complex in HNSCC can inhibit SDF-1 alpha induced phosphorylation and degradation of IkappaBalpha. PMID: 20695076
Interaction of calmodulin with Bcl10 modulates NF-kappaB activation. PMID: 20439115
A specific single nucleotide polymorphism in the BCL10 gene may be responsible for the tumorigenesis of intracranial germinomas in Japanese individuals. PMID: 19690445
Results suggest that the NFkappaB regulator BCL10 is an IL-2-independent STAT5 target gene. PMID: 19709433
These findings indicate an upstream signaling role for BCL10, in addition to its effects on IKKgamma, the regulatory component of the IKK signalosome, and a requirement for BCL10 in both canonical and noncanonical pathways of NF-kappaB activation. PMID: 19897484
vimplicated in apoptosis, and it has been suggested that mutated forms gain oncogenic activity. The occurrence of genomic BCL10 mutations in gastric MALT-type lymphomas was investigated. PMID: 11830492
Mutations, relatively common in lymphomas, are extremely rare in malignant cartilaginous tumors. PMID: 11836626
REVIEW: Genetic alterations involving BCL10 underlying the pathogenesis of MALT lymphoma PMID: 11960389
There is a lack of BCL10 mRNA mutation in lymphold malignancies. PMID: 12017308
association of mutations with aberrant BCL10 localization in the nucleus in nasal NK/T-cell lymphomas PMID: 14523480
Bcl10 is post-translationally modified by Rip2 and has a role in T-cell signaling PMID: 14638696
Nuclear expression of BCL10 is unlikely to correlate with the API2-MALT1 fusion gene in ocular adnexal MALT lymphoma. PMID: 14674990
Together, these findings suggest that Bcl10 nuclear expression may modulate gene expression and Bcl10 is a potential transcriptional activator apart from its traditional roles that have been found. PMID: 15207693
BinCard inhibits BCL10-mediated activation of NF-kappa B. PMID: 15637807
The ability of Bcl10 expression to prevent B-cell antigen receptor-induced growth arrest and apoptosis of WEHI-231 cells was dependent on NF-kappaB activation. PMID: 15878976
nucleocytoplasmic shuttling of MALT1 and BCL10 complex may indicate that these molecules are involved not only in the nuclear factor kappaB (NF-kappaB) pathway but also in other biologic functions in lymphocytes PMID: 16123224

Hide All

Involvement in disease

Immunodeficiency 37 (IMD37); Lymphoma, mucosa-associated lymphoid type (MALTOMA)

Subcellular Location

Cytoplasm, perinuclear region. Membrane raft.

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 989

OMIM: 137245

KEGG: hsa:8915

STRING: 9606.ENSP00000271015

UniGene: Hs.193516

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA164531A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A431 whole cell lysate(30µg) All lanes: Bcl 10 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 26 kDa Observed band size: 31 kDa Exposure time：5min Overlay Peak curve showing BxPC-3 cells stained with CSB-RA164531A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

BCL10 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background