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BID Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA143705A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: HeLa whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), MCF-7 whole cell lysate(30µg), K562 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), HEK293 whole cell lysate(30µg)
    All lanes: BID antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Exposure time: 3min
  • IHC image of CSB-RA143705A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • Overlay Peak curve showing Hela cells stained with CSB-RA143705A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
P55957
Target Names
BID
Alternative Names
Apoptic death agonist antibody; Apoptotic death agonist BID antibody; BH3 interacting domain death agonist antibody; BH3 interacting domain death agonist p11 antibody; BH3 interacting domain death agonist p13 antibody; BH3 interacting domain death agonist p15 antibody; BH3-interacting domain death agonist p11 antibody; BID antibody; BID isoform ES(1b) antibody; BID isoform L(2) antibody; BID isoform Si6 antibody; BID_HUMAN antibody; Desmocollin type 4 antibody; FP497 antibody; Human BID coding sequence antibody; MGC15319 antibody; MGC42355 antibody; p11 BID antibody; p13 BID antibody; p15 BID antibody; p22 BID antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human BID
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
3C9
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, IHC, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

BID (BH3-interacting domain death agonist) serves as a critical mediator in the intrinsic apoptotic pathway, functioning as a molecular link between death receptor signaling and mitochondrial-mediated cell death. Upon cleavage by caspase-8, truncated BID translocates to mitochondria where it promotes cytochrome c release, making it an essential target for researchers investigating apoptosis regulation, cancer biology, and therapeutic resistance mechanisms.

This recombinant monoclonal antibody, generated from clone 3C9 in rabbit host, offers the reproducibility and consistency that demanding apoptosis research requires. Because the antibody sequence is defined and production occurs through recombinant expression, researchers can expect reliable performance across experiments and between lots—a significant advantage when building long-term datasets or conducting studies requiring precise quantification of BID expression levels.

Validation across multiple experimental platforms demonstrates this antibody's versatility in your workflow. Western blot analysis confirms specific detection of BID at the expected 22 kDa molecular weight across a diverse panel of human cell lines, including HeLa, HepG2, A549, PC-3, MCF-7, K562, Jurkat, and HEK293 lysates at 1:1000 dilution. This broad validation across epithelial, hepatic, and hematopoietic cell types provides confidence when working with various experimental models. Immunohistochemistry staining has been optimized in paraffin-embedded human liver cancer tissue at 1:100 dilution, while flow cytometry analysis in HeLa cells demonstrates clear positive signal separation from isotype controls.

Whether you are characterizing apoptotic pathway activation in cancer models, investigating BID cleavage dynamics, or examining expression patterns in tissue specimens, this antibody provides the specificity and application flexibility needed for rigorous apoptosis research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

BID Antibodies

BID Proteins

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Target Background

Function
Induces caspases and apoptosis. Counters the protective effect of BCL2.; Induces caspase activation and apoptosis. Allows the release of cytochrome c.; Induces ICE-like proteases and apoptosis.; Induces ICE-like proteases and apoptosis.; Does not induce apoptosis.; Induces ICE-like proteases and apoptosis.
Gene References into Functions
  1. results suggest that histone modifications in the promoter region of miR-500a may be responsible for the increased expression of miR-500a in Hepatocellular carcinoma (HCC), which promotes cancer progression by targeting BID, indicating that miR-500a may be a potential prognostic predictor and therapeutic target for HCC patients. PMID: 29969781
  2. The caspase-8/Bid/cytochrome c axis links signals from death receptors to mitochondrial reactive oxygen species production. PMID: 28888620
  3. administration of SMAC or BH3 mimetics following short-term paclitaxel treatment could be an effective therapeutic strategy for TNBC, while only BH3-mimetics could effectively overcome long-term paclitaxel resistance PMID: 28187446
  4. caspase-10 was dispensable for enhancement of cisplatin/LA-12 and TRAIL combination-induced cell death and stimulation of Bid cleavage PMID: 29182622
  5. an oligomeric arrangement of Bid, Bax, and possibly other members of the Bcl-2 family of proteins that form a self-propagating network that permeabilizes the outer-mitochondrial membrane. PMID: 27763642
  6. Combined therapy with Seliciclib((R)) and Belinostat((R)) results in eradication of non-small cell lung cancer via apoptosis induction and BID activation. PMID: 27461583
  7. Results from genome-wide DNA methylation, functional network analysis and pyrosequencing, show selective CpG sites (NOS1AP, BID, and GABRB1) differentially methylated in smokers and chronic obstructive pulmonary disease patients compared to nonsmokers. PMID: 28416970
  8. The adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system can effectively eliminate CD133+ ovarian cancer stem cells, representing a novel therapeutic strategy for the treatment of ovarian cancer. PMID: 27878291
  9. MiR-20a-directed regulation of BID in colorectal cancer. PMID: 28004114
  10. the results establish that cleavage by caspase 8 and the subsequent association with the outer mitochondrial membrane are two critical events that activate Bid during death receptor-mediated apoptosis. PMID: 27053107
  11. The results indicate that BID-independent pathways are responsible for FAS-dependent human islet cell death in Type 1 diabetes. PMID: 26758067
  12. The BID-MTCH2 axis regulates the differentiation/apoptosis of stem cells and mitochondrial metabolism. (Review) PMID: 26827940
  13. Data suggest, in models of spatial propagation of mitochondrial permeabilization during apoptosis, there appears to be requirement for cooperative signaling involving truncated-BID and ROS (reactive oxygen species) for efficient/ robust propagation. PMID: 26699404
  14. this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
  15. Tax confers apoptosis resistance to HTLV-1-infected T cells by suppressing the expression of Bim and Bid. PMID: 25522269
  16. EG also activated the death receptor-dependent pathway of apoptosis by enhancing the expression of caspases-8, -9, and -3 and the Bcl-2 interacting domain (Bid). PMID: 23109891
  17. Inhibition of NANOGP8 or NANOG enhances the cytotoxicity of BH3 mimetics. PMID: 25208882
  18. JNK1/2 regulate Bid by direct phosphorylation at Thr59. PMID: 25077544
  19. Association of BID gene polymorphisms with proteinuria of immunoglobulin A nephropathy PMID: 24621205
  20. The extrinsic apoptotic pathway and BID are activated in adenomas from NSAID-treated patients. NSAIDS only activate BID in cells with APC deficiency and ensuing c-Myc activation. PMID: 25368155
  21. Gli1 has a role in regulating the S-phase checkpoint in tumor cells via Bid protein, and its inhibition sensitizes to DNA topoisomerase 1 inhibitors PMID: 25253693
  22. Bid is phosphorylated during mitosis within its regulatory loop and sensitizes mitochondria for mitochondrial outer membrane permeabilization if mitotic exit is delayed. PMID: 24767991
  23. BID expression was found in 53.6% of gastric cancer patients. PMID: 24741635
  24. BID is associated with ossification of the posterior longitudinal ligament , and both the G allele of a missense SNP (rs8190315, Ser10Gly) and C allele of a synonymous SNP (rs2072392, Asp60Asp) are risk factors for the development of ossification PMID: 24398548
  25. Reduced Bid expression is associated with endometrial cancer. PMID: 24645842
  26. FTY720 induces apoptosis of chronic myelogenous leukemia cells via dual activation of BIM and BID and overcomes various types of resistance to tyrosine kinase inhibitors. PMID: 23851982
  27. structural details on the membrane-associated state of tBid and the functional implications of its membrane-associated BH3 domain PMID: 24158446
  28. GrH induced cell death via a Bcl-2-sensitive mitochondrial pathway without direct processing of Bid. PMID: 23352961
  29. Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid PMID: 23418437
  30. Three cathepsin D-specific cleavage sites in Bid, Phe24, Trp48, and Phe183, were identified. PMID: 22964611
  31. Patients with Parkinson disease have an activated Bid-mediated destructive signal pathway via tumor necrosis factor death receptor I (TNFRI) in the temporal cortex. PMID: 23019260
  32. Molecular basis of the interaction between proapoptotic truncated BID (tBID) protein and mitochondrial carrier homologue 2 (MTCH2) protein PMID: 22416135
  33. Results suggest that truncated BID specifically interacts with phosphatidylserine/cardiolipin and decreases membrane integrity without the aid of other pro-apoptotic proteins. PMID: 22189507
  34. 14-3-3 theta/tau and tBID have roles as predictive biomarkers of neoadjuvant chemotherapy resistance in breast cancer PMID: 22115752
  35. Decreased apoptosis and expression of Bid, increased level of Bcl-Xl may play some important roles in human jejunal stromal tumors, and Bcl-Xl/Bid ratio may be as a new potentially associated index. PMID: 22339673
  36. Bid engages a ROS-dependent, local intermitochondrial potentiation mechanism that amplifies the apoptotic signal as a wave PMID: 22393005
  37. critical for controlling cell viability regulated by IFN-alpha in ovarian adenocarcinoma cells PMID: 22130162
  38. chronic ethanol increases CYP2E1 activity in adipose, leading to Bid-mediated apoptosis and activation of complement via C1q PMID: 21856753
  39. show that due to the high affinity of BAX for BCL-2, BCL-w and A1, and of BAK for BCL-X(L), MCL-1 and A1, only a subset of BH3-only proteins, including BID, could be expected to free BAX or BAK from the antiapoptotic BCL-2 proteins to elicit apoptosis PMID: 21060336
  40. a direct role for the BH3 interacting domain death agonist acting at the level of the DNA damage sensor complex to amplify the Atr-directed cellular response to replicative damage PMID: 21113148
  41. Data show that the expression of apoptosis proteins caspase-3,-8,-9, and Bid in the RNP of the disc and the SNP was different in each patient. PMID: 21178828
  42. Cells stabilize active caspase-8 on the mitochondria in order to specifically target mitochondria-associated BID. PMID: 21072056
  43. When tBid was introduced into the HIV-1 LTR-based, Tat- and Rev-dependent transgene expression vector pLRed(INS)2R, very efficient induction of apoptosis was observed within 24 hours,but only in the presence of both HIV-1 regulatory proteins Tat and Rev. PMID: 21223573
  44. tBID and other BCL-2 proteins have a role in osteosarcoma [commentary] PMID: 19029793
  45. In rheumatoid arthritis fibroblast like synovial cells phosphorylation of Akt protects against Fas-induced apoptosis through inhibition of Bid cleavage. PMID: 20187936
  46. The interface between apoptosis initiation and execution by determining caspase-8 activation, Bid cleavage and mitochondrial engagement (onset of mitochondrial depolarisation) in individual HeLa, was analysed. PMID: 20356928
  47. Data revealed that the level of tBid expression in breast cancer cells were nearly 11 times more than normal cells because of the cancer specific promoters. PMID: 19918914
  48. Bid may serve as a critical integrating factor of the death receptor and mitochondrial pathway in alpha-tocopheryl succinate-mediated apoptosis. PMID: 19640637
  49. tBid-induced permeabilization of the outer membrane permits release of cytochrome c and Smac/DIABLO from all domains of intermembrane space. tBid-induced loss of DeltaPsi(m) occurs after cytochrome c release and reflects impairment of oxidative metabolism PMID: 11741882
  50. Cytochrome c release upon Fas receptor activation on translocation of full-length bid PMID: 11790791

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Subcellular Location
Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane.; [BH3-interacting domain death agonist p15]: Mitochondrion membrane.; [BH3-interacting domain death agonist p13]: Mitochondrion membrane.; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm.; [Isoform 2]: Mitochondrion membrane.
Tissue Specificity
[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level).; [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level).; [Isoform 4]: Expressed in lung and pancreas (at protein level).
Database Links

HGNC: 1050

OMIM: 601997

KEGG: hsa:637

STRING: 9606.ENSP00000318822

UniGene: Hs.591054

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Related pathways
p53 signaling pathway Apoptosis Necroptosis Natural killer cell mediated cytotoxicity
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