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BH3-interacting domain death agonist is a protein in humans that is encoded by BID gene. The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
The following BID reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
BID Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA002698HA01HU | BID Antibody |
Human | ELISA, IHC |
| CSB-PA002698HC01HU | BID Antibody, FITC conjugated |
Human | |
| CSB-PA002698GA01HU | BID Antibody |
Human | ELISA,WB,IHC |
| CSB-PA000015 | BID Antibody |
Human | WB, ELISA |
| CSB-PA000994 | BID Antibody |
Human,Mouse | WB, IHC, ELISA |
| CSB-PA040302 | Phospho-BID (S78) Antibody |
Human,Mouse | IHC, ELISA |
| CSB-PA787438 | BID Antibody |
Human | ELISA,WB,IHC |
| CSB-PA225375 | BID Antibody |
Human | ELISA,WB,IHC |
BID Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP002698MO CSB-EP002698MO CSB-BP002698MO CSB-MP002698MO CSB-EP002698MO-B |
Recombinant Mouse BH3-interacting domain death agonist(Bid) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BID Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP002698HU CSB-BP002698HU CSB-MP002698HU CSB-EP002698HU-B |
Recombinant Human BH3-interacting domain death agonist(BID) |
Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-EP002698HU | Recombinant Human BH3-interacting domain death agonist(BID) |
E.coli |
BID Proteins for Sus scrofa (Pig)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP678611PI CSB-EP678611PI CSB-BP678611PI CSB-MP678611PI CSB-EP678611PI-B |
Recombinant Pig BH3-interacting domain death agonist(BID) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BID Proteins for Gallus gallus (Chicken)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP814259CH CSB-EP814259CH CSB-BP814259CH CSB-MP814259CH CSB-EP814259CH-B |
Recombinant Chicken BH3-interacting domain death agonist(BID) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
BID Proteins for Rattus norvegicus (Rat)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP867557RA CSB-EP867557RA CSB-BP867557RA CSB-MP867557RA CSB-EP867557RA-B |
Recombinant Rat BH3-interacting domain death agonist(Bid) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
The BID gene encodes the BH3-interacting domain death agonist (BID), a pro-apoptotic member of the Bcl-2 protein family [1]. BID possesses a single BH3 (Bcl-2 homology 3) domain and lacks a C-terminal transmembrane domain for the outer mitochondrial membrane[1]. BID is localized in the cytosolic or on the plasma membrane. BID is inactive, but becomes activated after Caspase-8 cleavage [2][3][4]. Truncated BID (tBID) may expose the BH3 motif buried in the uncleaved molecule. Protease-cleaved BID subsequently translocates to mitochondria where it induces permeabilization of the mitochondrial outer membrane (MOM) dependent on the pro-apoptotic proteins Bax and/or Bak. BID, therefore, acts as a sentinel for protease-mediated death signals [7]. Mutagenesis study indicated that the BH3 domain is required for the apoptotic activity of BID since BID with an intact BH3 domain may retain interaction with BAX [4][5]. Cells generate pro-apoptotic proteins with a single active domain called BH3 in stress or damage. BID acts as an activator to induce the overexpression of effector pro-apoptotic proteins such as BAX (BCL2-associated X) and BAK (BCL2 antagonist/killer 1) [6]. The production of BAX and BAK further leads to cytochrome c release into the cytoplasm, following caspase activation and apoptosis [6].
[1] Wang K, Yin XM, et al. BID: a novel BH3 domain-only death agonist [J]. Genes Dev. 1996, 10 (22): 2859-9.
[2] Gross A., Yin X.-M., et al. Caspase cleaved BID targets mitochondria and is required for cytochrome c release, while BCL-XL prevents this release but not TNF-R1/Fas death [J]. J. Biol. Chem. 1999; 274: 1156-1163.
[3] Li H., Zhu H., et al. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis [J]. Cell. 1998; 94: 491-501.
[4] Luo X., Budihardjo I. et al. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors [J]. Cell. 1998; 94: 481-490.
[5] Wang K., Gross A., et al. Mutagenesis of the BH3 domain of BAX identifies residues critical for dimerization and killing [J]. Mol. Cell. Biol. 1998; 18: 6083-6089.
[6] Korsmeyer SJ, Wei MC, et al. Pro-apoptotic cascade activates BID, which oligomerizes BAK or BAX into pores that result in the release of cytochrome c [J]. Cell Death Differ 2001; 7: 1166-173.
