Product Details

Uniprot No.

Target Names

Alternative Names

API 1 antibody; API1 antibody; Apoptosis inhibitor 1 antibody; Baculoviral IAP repeat containing 2 antibody; Baculoviral IAP repeat containing protein 2 antibody; Baculoviral IAP repeat-containing protein 2 antibody; BIRC 2 antibody; BIRC2 antibody; BIRC2_HUMAN antibody; C IAP1 antibody; C-IAP1 antibody; Cellular inhibitor of apoptosis 1 antibody; cellular inhibitor of apoptosis protein 1 antibody; cIAP 1 antibody; cIAP1 antibody; HIAP 2 antibody; HIAP-2 antibody; HIAP2 antibody; IAP 2 antibody; IAP homolog B antibody; IAP-2 antibody; IAP2 antibody; Inhibitor of apoptosis protein 2 antibody; MIHB antibody; NFR2 TRAF signalling complex protein antibody; RING finger protein 48 antibody; RNF 48 antibody; RNF48 antibody; TNFR2 TRAF signaling complex protein 2 antibody; TNFR2-TRAF-signaling complex protein 2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human BIRC2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14B8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BIRC2, also known as cellular inhibitor of apoptosis protein 1 (cIAP1), plays a central role in regulating programmed cell death and cell survival signaling pathways. As a member of the inhibitor of apoptosis protein family, BIRC2 functions as an E3 ubiquitin ligase that modulates TNF receptor signaling and NF-κB activation, making it a key target for researchers investigating apoptosis regulation, cancer biology, and inflammatory responses.

This recombinant monoclonal antibody, clone 14B8, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation studies demonstrate reliable performance across multiple applications. In western blot analysis, the antibody detects BIRC2 at the expected 70 kDa molecular weight in human cell lines including U-251MG glioblastoma and COLO-205 colorectal adenocarcinoma cells, with recommended dilutions ranging from 1:500 to 1:5000. Flow cytometry validation using HeLa cells confirms utility for single-cell analysis of intracellular BIRC2 expression, with clear separation between specific staining and isotype control populations at dilutions between 1:50 and 1:200.

The antibody is supplied in a stabilized liquid format containing glycerol for convenient long-term storage at -20°C or -80°C. For researchers exploring apoptotic pathways, tumor cell survival mechanisms, or therapeutic targeting of IAP family proteins, this antibody provides a dependable tool for characterizing BIRC2 expression and function in human experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO, IKBKE and MXD1/MAD1. Can also function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle.

Gene References into Functions

study provides an interesting example using chemical biological approaches for determining distinct biological consequences from inhibiting vs. activating an E3 ubiquitin ligase and suggests a potential broad therapeutic strategy for targeting c-MYC in cancer treatment by pharmacologically modulating cIAP1 E3 ligase activity. PMID: 30181285
NAIP expression is most abundant in M2 macrophages, while cIAP1 and cIAP2 show an inverse pattern of expression in polarized cells, cIAP2 is preferentially expressed in M1-macrophages and cIAP1 in M2-macrophages. IAP antagonist treatment of resting M0 macrophages preceding polarization stimulation, induced upregulation of NAIP in M2 and downregulation of cIAP1 in M1 and M2 but an induction of cIAP2 in M1 macrophages. PMID: 29518103
we conclude that DMBT1 by binding with CRNDE and c-IAP1 associated with PI3K-AKT pathway is crucial for GBC carcinogenesis, and targeting this pathway may be pivotal in the treatment of GBC. PMID: 27637083
These results reveal an additional level of regulation of the stability and the activity of E2F1 by a non-degradative K63-poly-ubiquitination and uncover a novel function for the E3-ubiquitin ligase cIAP1. PMID: 28542143
High cIAP1 expression is associated with colorectal cancer. PMID: 27014915
the cytoplasmic retinoic acid receptor gamma (RARgamma) controls receptor-interacting protein kinase 1 (RIP1)-initiated cell death when cellular inhibitor of apoptosis (cIAP) activity is blocked. PMID: 28871172
High cIAP1 expression is associated with Lung Tumorigenesis. PMID: 27197231
These data suggest that molluscum contagiosum virus MC159 competitively binds to NEMO to prevent cIAP1-induced NEMO polyubiquitination. PMID: 28515292
The baseline tumor necrosis factor tumor (TNFalpha) expression correlates with the sensitivity to Inhibitors of apoptosis proteins (IAPs) antagonist T-3256336. PMID: 27608596
cIAP1-induced mitophagy led to dysfunctional mitochondria that resulted in abrogation of mitochondrial oxygen consumption rate along with the decrease in ATP levels. The ubiquitination of cIAP1 was found to be the critical regulator of mitophagy. PMID: 27693792
Overexpression of cIAP1 is associated with glioma. PMID: 26666816
High expression of cIAP1 is associated with triple-negative breast cancer. PMID: 26733177
cIAP1 and cIAP2 mediate BCL10 ubiquitination essential for BCR-dependent NF-kB activity in the ABC subtype of DLBCL. cIAP1/2 attach K63-linked polyubiquitin chains on themselves and on BCL10, recruiting IKK and LUBAC essential for IKK activation. PMID: 27070702
AZD5582 draws Mcl-1 down-regulation for induction of apoptosis through targeting of cIAP1 and XIAP in human pancreatic cancer PMID: 26314849
Data indicate that Smac/DIABLO showed an inverse correlation with inhibitor of apoptosis proteins XIAP, cIAP-1 and cIAP-2. PMID: 25549803
Data show that ectopic expression of interferon regulatory factor 1 (IRF-1) reduces NF-kappa B activity and suppresses TNF receptor-associated factor 2 (TRAF2) and inhibitor of apoptosis 1 protein (cIAP1) expression in breast cancer cells. PMID: 26011589
cIAP1 is associated with tumor progression in human ovarian cancer. PMID: 26168135
The E3 ubiquitin ligase activity of XIAP and cIAP1 activates NFkappaB signalling, leading to the direct binding of p65 to the promoter of Beclin 1 and to its transcriptional activation and induction of autophagy. PMID: 25669656
CIAP1 and cIAP2 represent novel therapeutic targets for the prevention of spontaneous preterm birth. PMID: 25046208
Data suggest that the seventh zinc finger motif of DNA-binding protein A20 plays important role in NFkappaB-mediated apoptosis induced by tumor necrosis factor-alpha; A20 appears to bind and thus down-regulate inhibitor of apoptosis proteins cIAP1/2. PMID: 25911380
these findings suggest that GDC-0152 results in human leukemia apoptosis through caspase-dependent mechanisms involving down-regulation of IAPs and inhibition of PI3K/Akt signaling. PMID: 25273171
Results identify IGF2BP1 as a critical translational regulator of cIAP1-mediated apoptotic resistance in rhabdomyosarcoma. PMID: 24704827
Survivin affected the stability of cIAP1 through binding, contributing to cell sensitivity to antineoplastic drug YM155. PMID: 25635055
cIAP1 and cIAP2 expression is increased in placenta from women with pre-existing maternal obesity and in response to treatment with pro-inflammatory cytokines PMID: 25113061
XIAP, cIAP1, and cIAP2, members of inhibitor of apoptosis (IAP) proteins, are critical regulators of cell death and survival and are attractive targets for new cancer therapy PMID: 24841289
Although motions within each interface of the 'closed' monomer are insufficient to activate cIAP1, they enable associations with catalytic partners and activation factors. PMID: 25383668
cIAP1 undergoes a dramatic conformational change during activation that is now shown to be due to the dynamic and metastable nature of the closed form of the enzyme. PMID: 25469840
Data suggest IAP1 is involved in pancreatic beta cell survival during endoplasmic reticulum stress; switching between IAP1 and transcription factor CHOP in unfolded protein response allows cells to survive or kills cells through apoptosis. [REVIEW] PMID: 24559922
we show that cIAP1 regulates TNF-induced actin cytoskeleton reorganization through a cdc42-dependentpathway PMID: 24276241
ARC is regulated via BIRC2/MAP3K14 signalling and its overexpression in AML or MSCs can function as a resistant factor to birinapant-induced leukaemia cell death. PMID: 25079338
study demonstrate EndoG interacts with cIAP1; results indicate IAPs interact and ubiquitinate EndoG via K63-mediated isopeptide linkages without affecting EndoG levels and EndoG-mediated cell death, suggesting EndoG ubiquitination by IAPs may serve as a regulatory signal independent of proteasomal degradation PMID: 25139236
cIAPs constitutively downregulate PACS-2 by polyubiquitination and proteasomal degradation, thereby restraining TRAIL-induced killing of liver cancer cells PMID: 24633224
Selenite caused CYLD upregulation via LEF1 and cIAP downregulation, both of which contribute to the degradation of ubiquitin chains on RIP1 and subsequent caspase-8 activation and colorectal tumor cell apoptosis. PMID: 24577083
Ubiquitin-dependent regulation of MEKK2/3-MEK5-ERK5 signaling module by XIAP and cIAP1.cIAP1 role in the physical and functional disassembly of ERK5-MAPK module and human muscle cell differentiation. PMID: 24975362
higher expression in the ovarian endometriomas PMID: 24382102
we note that the compounds that sensitize cancer cells to TRAIL are the most efficacious in binding to X-linked IAP, and in inducing cellular-IAP (cIAP)-1 and cIAP-2 degradation PMID: 24194568
Identify xIAP and cIAP1 as molecular targets of ceramide and show ceramide analog LCL85 is an effective sensitizer in overcoming resistance of metastatic colon and breast cancers to apoptosis induction to suppress metastasis in vivo. PMID: 24422988
Data indicate that silencing of IAPs, IAP1 IAP2 and XIAP, reduced the TRAP-evoked RhoA activation. PMID: 24371121
cisplatin significantly triggered the proteasomal degradation of cellular inhibitor of apoptosis protein 1 and 2 (c-IAP1 and c-IAP2), and X-linked inhibitor of apoptosis (XIAP) in a ROS-dependent manner PMID: 24425875
Data indicate the discovery of benzodiazepinone 36, a selective XIAP BIR2 inhibitors. PMID: 24093940
Data indicate that a potent BIR2-selective inhibitor with in vivo pharmacokinetic properties which potentiates apoptotic signaling. PMID: 24083782
palmitate did not decrease cIAP-1 and cIAP-2 mRNA expression in the cells PMID: 24008361
The cIAP1 expression was decreased by siRNA silencing of Foxa2, but increased by Foxa2-expressing vectors. PMID: 23275033
BIRC2 and BIRC3 act as a molecular brake to rein in activation of the JNK signalling pathway and to fine-tune NF-kappaB and JNK signalling to ensure transcriptional responses are appropriately matched to extracellular inputs. PMID: 23250032
Data suggest that inhibitor of apoptosis (IAP) antagonist-based cancer treatment may be compromised by osteoporosis and enhanced skeletal metastasis, which may be prevented by antiresorptive agents. PMID: 23269702
The 3'untranslated region of BIRC2 contains a cis-acting element that decreases stability of the message but increase protein expression PMID: 23605047
These results suggest that OTUB1 regulates NF-kappaB and MAPK signalling pathways and TNF-dependent cell death by modulating c-IAP1 stability. PMID: 23524849
a molecular mechanism for cIAP1's regulation in the NOD2 signaling pathway PMID: 23109427
c-IAP1 acts on both secretion of PCSK9 and its lysosomal localization. The novel pathway described here will open new avenues for exploring novel disease treatments. PMID: 23085658
IAP inhibitors and lexatumumab synergistically trigger apoptosis in a RIP1-dependent but TNFalpha-independent manner in RMS cells PMID: 22927431

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Subcellular Location

Cytoplasm. Nucleus. Note=Agents that induce either the extrinsic or intrinsic apoptotic pathways promote its redistribution from the nuclear compartment to the cytoplasmic compartment. Associated with the midbody in telophase cells, and found diffusely in the nucleus of interphase cells.

Protein Families

IAP family

Tissue Specificity

Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes.

Database Links

HGNC: 590

OMIM: 601712

KEGG: hsa:329

STRING: 9606.ENSP00000227758

UniGene: Hs.696238

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Code	CSB-RA583495A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: U-251MG whole cell lysate(30µg), COLO-205 whole cell lysate(30µg) All lanes: BIRC2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time：2min Overlay Peak curve showing Hela cells stained with CSB-RA583495A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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BIRC2 Recombinant Monoclonal Antibody

Product Details

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Target Background