Product Details

Uniprot No.

Target Names

Alternative Names

BCL2 Adenovirus E1B 19kDa Interacting Protein 3 antibody; BCL2/adenovirus E1B 19 kDa protein interacting protein 3 antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 antibody; BNIP 3 antibody; BNIP3 antibody; BNIP3_HUMAN antibody; NIP 3 antibody; NIP3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human BNIP3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5E11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3) is a pro-apoptotic BH3-only member of the BCL2 family that plays a central role in hypoxia-induced cell death and mitophagy. As a key mediator of cellular responses to oxygen deprivation, BNIP3 has become an important focus in cancer biology, cardiovascular research, and studies of metabolic stress. Its expression is tightly regulated by hypoxia-inducible factors, making it a valuable marker for investigating tumor microenvironments and ischemic conditions.

This recombinant rabbit monoclonal antibody (clone 5E11) offers the reproducibility and consistency that demanding research requires. Generated against a synthetic peptide derived from human BNIP3, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. Affinity purification further enhances performance by reducing background signal.

Validation data demonstrate reliable detection across multiple experimental platforms. Western blot analysis of U-251MG glioblastoma cell lysates reveals a clean band at the predicted 22 kDa molecular weight, confirming accurate target recognition. For tissue-based studies, immunohistochemistry has been validated in paraffin-embedded human kidney and prostate cancer sections, providing researchers with confidence when examining BNIP3 expression in both normal and malignant contexts. Flow cytometry validation using 786-O renal carcinoma cells demonstrates clear positive signal separation from isotype controls, supporting intracellular detection workflows.

Whether you are investigating hypoxia signaling pathways, characterizing mitophagy mechanisms, or exploring BNIP3 as a potential biomarker in oncology research, this antibody provides the flexibility and reliability needed for rigorous cell biology investigations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.

Gene References into Functions

BNIP3 was mainly localized in the cytoplasm, and high expression of BNIP3 accounted for 31.9% (15/47) of the uveal melanoma patients in our study. High expression of BNIP3 was demonstrated to be significantly associated with more pigment (P=0.018) and deeper scleral invasion (P=0.013). PMID: 29982263
lncRNA HULC up-regulated BNIP3. PMID: 29803927
TUG1 overexpression aggravates hypoxia injury of cardiomyocytes by regulating miR-145-5p-Bnip3 axis to activate Wnt/beta-catenin pathways. PMID: 29207102
In ESCC cells, although BNIP3-induced autophagy is protective, BNIP3 exerts a pro-death function under hypoxia. PMID: 28859361
Silencing of BNIP3 suppressed free fatty acid synthesis in mesenchymal stem cells. BNIP3 induction by hypoxia stimulates FASN-dependent free fatty acid production. PMID: 28704726
Apoptosis, but not autophagy, that is induced via p21-activated BNIP3 expression accounts for the efficacy of ICMT inhibition in sensitive pancreatic cancer cells in both in vitro and in vivo models. In contrast, cells resistant to ICMT inhibition demonstrated no mitochondria dysfunction or p21 signaling changes under ICMT suppression PMID: 28167504
BNIP3 is a significant contributor to the pathogenesis of inflammation-induced heart failure pathologies [review] PMID: 27112557
The authors find that KDM3A promotes anoikis through transcriptional activation of BNIP3 and BNIP3L, which encode pro-apoptotic proteins. PMID: 27472901
BNIP3-induced autophagy. PMID: 28151469
the results suggest that BNIP3 plays a vital role in regulating PINK1 mitochondrial outer membrane localization, the proteolytic process of PINK1 and PINK1/parkin-mediated mitophagy under physiological conditions. PMID: 27528605
High BNIP3 expression is associated with chronic myelogenous leukemia. PMID: 27592257
Results suggest that changes in mitochondrial morphology and transmembrane potential, induced by mutant htt protein, are dependent and linked to BNip3 and not to Bax/Bak activation. PMID: 26358776
Hypoxia-induced autophagy contributes to the invasion of salivary adenoid cystic carcinoma through the HIF-1alpha/BNIP3 signaling pathway. PMID: 26323347
The data indicated that BNIP3 plays a vital role in the tumorigenesis of adenoid cystic carcinoma and could be a new target for gene therapy of adenoid cystic carcinoma. PMID: 25769455
BNIP3 deletion can be used as a prognostic marker of tumor progression to metastasis in human triple-negative breast cancer PMID: 26232272
BNIP3 expression was found to be regulated by Sp3 in prostate cancer. PMID: 26012884
phosphorylation of these C-terminal BNIP3 residues blocks cell death without preventing autophagy, providing evidence that the two functional roles of BNIP3 can be regulated independently PMID: 26102349
Bnip3 dual-functionality and crosstalk between mitophagy and apoptosis pathways is presented here. PMID: 26253153
these findings revealed that silibinin induced autophagic cell death through ROS-dependent mitochondrial dysfunction and ATP depletion involving BNIP3 in MCF7 cells. PMID: 25891311
The findings of the present study reveal a novel survival pathway that functionally couples the unique glycolytic phenotype in cancer cells to hypoxia resistance via a PDK2-dependent mechanism that switches Bnip3 from cell death to survival. PMID: 26416963
Mevalonate depletion through HMG-CoA reductase inhibition impairs the viability of primary human mesenchymal stells via NF-kappaB/Bnip3 signaling. PMID: 25547946
protective role of BNIP3 in the form of autophagy inducer to reduce the misfolded proteins directly and prevention of neurodegenerative diseases indirectly PMID: 24928088
our results bring the evidence of a mechanism that links apoptosis and ROS-induced BNIP3 expression in MG-63 cells with bacalein treatment and suggest that baicalein has a good potential as an anti-osteosarcoma drug. PMID: 25618603
Results show that BNIP3 interacts with the voltage-dependent anion channel (VDAC) to directly induce mitochondrial release and nuclear translocation of EndoG. PMID: 25436615
AT101 caused caspase-independent, non-apoptotic malignant peripheral nerve sheath tumor cell death, which was accompanied by autophagy and was mediated through HIF-1alpha induced expression of the atypical BH3-only protein BNIP3. PMID: 24824755
BNIP3 shows low levels of promoter hypermethylation in invasive breast cancer, mostly lower than the proposed 15% threshold that supposedly discriminates "true" from background methylation. PMID: 20978322
Low BNIP3 expression is associated with renal cancer. PMID: 23851496
BNIP3 has a protective effect against UVB-induced apoptosis in keratinocytes PMID: 24402046
the impact of Glutamate carboxypeptidase II (GCPII) haplotypes on the expression of PSMA, BNIP3, Ec-SOD, GSTP1 and RASSF1 genes were elucidated to understand the epigenetic basis of oxidative stress and prostate cancer risk. PMID: 23979608
The upregulation of miR-210 directly suppresses BNIP3 expression to maintain the survival of NPCs under hypoxia. PMID: 23688833
This paper aims to summarize the existing body of knowledge related to the role of BNIP3 in autophagy, as well as the importance of this process in tumorigenesis. PMID: 23667095
BNIP3 is initially silenced by methylation, but upon re-expression remains under predominant control of Ras and the related MEK/MAPK pathway (as presented in DLD-1 and HT-29 cells). PMID: 24211581
our data show for the first time that glucose availability significantly affects the hypoxia-induced HIF-1/BNIP3 response, and in particular glucose absence results in enhancing the oxidative phosphorylation rate. PMID: 23538299
Both expression level and activity of DNMT1 were inversely correlated with the expression level of BNIP3 in colon carcinoma cells after treatment with chemotherapeutic agents and radiation PMID: 23364257
ULK1-dependent autophagy degrades BNIP3 via MTORC1 and AMPK PMID: 23291726
The accelerated autophagic status in non-small cell lung carcinoma is unrelated to Beclin 1 and BNIP3 expression, but does show significant association with Bcl-2 reactivity. PMID: 23216071
results suggest aberrations in one-carbon metabolism appear to induce altered gene expression of EC-SOD, GSTP1, and BNIP3, and thus contribute to the increased oxidative stress and increased susceptibility to coronary artery disease PMID: 23160801
pro-survival Bcl-x(L) positively regulated Bnip3 binding to LC3B, sequestration, and mitochondrial autophagy, further supporting an anti-apoptotic role for Bnip3-induced mitophagy PMID: 23209295
BNIP3 expression induced autophagosome accumulation with lysosome consumption in cardiomyocytes. PMID: 22302006
BNIP3 expression and TGM2 expression are independent prognostic factors in laryngeal SCC patients receiving postoperative radiotherapy. PMID: 22458929
Bnip3 induced removal of both ER (ERphagy) and mitochondria (mitophagy) via autophagy. The clearance of these organelles was mediated in part via binding of Bnip3 to LC3 on the autophagosome. PMID: 22505714
The expression of both HIF-1alpha and VEGF were not significantly increased in BNIP3 positive clear cell renal cell carcinoma in comparison with BNIP3 negative tumors. PMID: 22455137
The physical interaction of Mieap, BNIP3 and NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. PMID: 22292033
The detection of genes selectively methylated in high-grade Intraductal papillary mucinous neoplasms (IPMNs) such as BNIP3 may have use in the clinical evaluation of IPMNs PMID: 22173550
MTRR A66G and cSHMT C1420T polymorphisms influence CpG island methylator phenotype of BNIP3, thus epigenetically regulating BNIP3 in breast cancer PMID: 21987236
The BNIP3 promoter is methylated in both human hepatoma and colon carcinoma cells and tumor specimens. PMID: 21911457
proapoptotic regulator Bcl2/adenovirus EIB 19 kDa-interacting protein 3 has an effect on radiosensitivity of cervical cancer PMID: 21711117
Hypermethylation of BNIP3 gene is associated with advanced gastric cancer. PMID: 21409489
p53 suppressed BNIP3 expression by directly binding to the p53-response element motif and recruiting corepressor mSin3a to the BNIP3 promoter. PMID: 21792176
DNA methylation of the BNIP3 promoter is responsible for the inhibition of BNIP3 induction PMID: 21573703

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Subcellular Location

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note=Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Protein Families

NIP3 family

Database Links

HGNC: 1084

OMIM: 603293

KEGG: hsa:664

STRING: 9606.ENSP00000357625

UniGene: Hs.144873

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Code	CSB-RA552618A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: U-251MG whole cell lysate(30µg) All lanes:BNIP3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time：2min IHC image of CSB-RA552618A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA552618A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA552618A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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BNIP3 Recombinant Monoclonal Antibody

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Target Background