BTN3A1 Recombinant Monoclonal Antibody

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Datasheet

Code	CSB-RA002873MA2HU
Size	$49.9
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Image	IHC image of CSB-RA002873MA2HU diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA002873MA2HU at 1:30 ,counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific.
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Target Background

Product Details

Uniprot No.

O00481

Target Names

BTN3A1

Alternative Names

BT3A1_HUMAN antibody; BTN3A1 antibody; Butyrophilin subfamily 3 member A1 antibody; CD277 antibody

Species Reactivity

Human

Immunogen

Recombinant Human BTN3A1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

18F9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BTN3A1, also known as CD277, is a member of the butyrophilin subfamily that plays a pivotal role in immune regulation and γδ T cell activation. This transmembrane protein has garnered significant research attention for its involvement in phosphoantigen sensing and its emerging importance in cancer immunotherapy, where it serves as a critical mediator of immune surveillance mechanisms.

This recombinant monoclonal antibody, clone 18F9, offers researchers the consistency and reliability that only sequence-defined production can provide. Generated against recombinant human BTN3A1 protein and expressed as a human IgG1 isotype, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and reproducibility across experiments. The affinity-chromatography purification ensures high purity for demanding applications.

Validation studies demonstrate robust performance across multiple immunodetection platforms. In immunohistochemistry, this antibody successfully detected BTN3A1 in paraffin-embedded human liver cancer tissue using citrate buffer antigen retrieval at dilutions of 1:50 to 1:200, revealing expression patterns relevant to tumor immunology research. Immunofluorescence applications have been validated in HeLa cells, where the antibody produced clear membrane and cytoplasmic staining patterns at 1:30 dilution, with DAPI counterstaining confirming cellular localization. The antibody is also suitable for ELISA-based detection methods.

Supplied in a stabilized liquid format containing 50% glycerol in PBS with Proclin 300 preservative, this antibody is ready for immediate use in your experimental workflows. For researchers investigating γδ T cell biology, tumor immunology, or butyrophilin family function in human samples, this antibody provides a dependable tool for characterizing BTN3A1 expression and distribution.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.

Gene References into Functions

Microtubule-associated protein 4 (MAP4) controls the dynein-dependent transport of BTN3A1 in response to nucleic acid stimulation, thereby identifying MAP4 as an upstream regulator of BTN3A1. Thus, the depletion of either MAP4 or BTN3A1 impairs cytosolic DNA- or RNA-mediated type I IFN responses. PMID: 27911820
results show that ligand binding to BTN3A1 induces a conformational change within the intracellular domain that involves the JM region and is required for full activation. PMID: 28705810
findings show that changes in the juxtamembrane domain of BTN3A1, but not its transmembrane domain, induce a markedly enhanced or reduced gammadelta T cell reactivity PMID: 28461569
These findings support intracellular sensing of prenyl pyrophosphates by BTN3A1 rather than extracellular presentation. PMID: 26475929
Human gamma-delta T cells are activated by cytosolic interactions of BTN3A1 with soluble phosphoantigens and the cytoskeletal adaptor periplakin. PMID: 25637025
evidence that gene(s) on Chr6 in addition to BTN3A1 are mandatory for PAg-mediated activation of Vgamma9Vdelta2 T cells. PMID: 24890657
Ligand binding to the BTN3A1 B30.2 domain affects residues in the juxtamembrane region, suggesting ligand-induced conformational change. PMID: 25065532
These studies demonstrate that internal sensing of changes in pAg metabolite concentrations by BTN3A1 molecules is a critical step in Vgamma9Vdelta2 T cell detection of infection and tumorigenesis. PMID: 24703779
BTN3A1 represents an antigen-presenting molecule required for the activation of Vgamma9Vdelta2 T cells. PMID: 23872678
investigation of role of CD277 in activation/inactivation of T-lymphocytes: Data indicate that modulation of CD277 interaction (with agonists or blocking antibodies) with T-cell antigen receptor can modulate activation/inactivation of T-lymphocytes. PMID: 22767497
BTN3A1 is necessary for Vgamma9Vdelta2 activation and begin to unravel the extracellular events that occur during stimulation through the Vgamma9Vdelta2 T cell receptor. PMID: 22846996
CD277 triggering is not involved in CD16- or NKp46-induced NK cell activation. PMID: 21918970
Results point to a role for CD277 up-regulated by microenvironmental signals in the acquisition of a regulatory phenotype by ovarian tumor-associated myeloid cells. PMID: 21113407

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily, BTN/MOG family

Tissue Specificity

Detected on T-cells, natural killer cells, dendritic cells and macrophages (at protein level). Ubiquitous. Highly expressed in heart, pancreas and lung, Moderately expressed in placenta, liver and muscle.

Database Links

HGNC: 1138

OMIM: 613593

KEGG: hsa:11119

STRING: 9606.ENSP00000289361

UniGene: Hs.191510

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BTN3A1 (CD277) Target: γδ T Cell Immune Recognition Mechanism and Cancer Immunotherapy Research Progress Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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