Product Details

Uniprot No.

P27824

Target Names

CANX

Alternative Names

Calnexin (IP90) (Major histocompatibility complex class I antigen-binding protein p88) (p90), CANX

Species Reactivity

Human

Immunogen

A synthesized peptide from human CANX protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11C2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Calnexin serves as a critical molecular chaperone within the endoplasmic reticulum, where it plays an essential role in the quality control of newly synthesized glycoproteins. This type I integral membrane protein ensures proper folding and assembly of proteins destined for secretion or membrane insertion, making it a key marker for ER studies and an important target in research exploring protein folding disorders, ER stress responses, and cellular homeostasis.

This recombinant monoclonal antibody, clone 11C2, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a sequence-defined rabbit IgG format, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The antibody was raised against a synthetic peptide derived from human CANX and purified by affinity chromatography to ensure high specificity.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis of HEK293 whole cell lysate reveals a distinct band at approximately 94 kDa, which differs from the predicted 68 kDa molecular weight due to the extensive glycosylation characteristic of this ER-resident chaperone. Immunofluorescence staining in HeLa cells shows clear ER localization patterns, while flow cytometry analysis confirms robust detection with excellent signal separation from isotype controls.

Whether you're investigating ER stress pathways, studying the unfolded protein response, or characterizing protein trafficking mechanisms, this antibody provides reliable detection of calnexin in human samples across western blotting, immunofluorescence, and flow cytometry applications, supporting both qualitative localization studies and quantitative expression analyses.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

Gene References into Functions

In summary, ER retention of pathogenic VLDLR mutants involves binding to calnexin, elevated endoplasmic reticulum stress, and delayed degradation which is dependent on SEL1L. PMID: 29371607
Increased tumour protein levels of calnexin may be of prognostic significance in colorectal cancer, and calnexin may represent a potential target for future therapies. PMID: 27369741
FUNDC1 integrates mitochondrial fission and mitophagy at the interface of the endoplasmic reticulum-mitochondrial contact site by working in concert with DRP1 and calnexin under hypoxic conditions in mammalian cells. PMID: 27145933
Results of further analyses by using a CNX mutant imply that ERp29 and ERp57 recognize the same domain of CNX, whereas the mode of interaction with CNX might be somewhat different between them. PMID: 28456374
Inhibit interaction between HIV-1 Nef and Calnexin to reverse HIV-induced lipid accumulation and prevent atherosclerosis. PMID: 27470515
Endogenous NOX4 forms macromolecular complexes with calnexin, which are needed for the proper maturation, processing, and function of NOX4 in the endoplasmic reticulum. PMID: 26861875
To study principles underlying dynamics and regulation of palmitoylation, the ER chaperone calnexin, which requires dual palmitoylation for function, was investigated. PMID: 26900856
These observations point at a previously unrecognized contribution of calnexin to the retention of NMT1 at the ER membrane. PMID: 26603938
Charcot-Marie-Tooth disease-related PMP22 is trapped in the endoplasmic reticulum by calnexin-dependent retention and Rer1-mediated early Golgi retrieval systems and partly degraded by the Hrd1-mediated endoplasmic reticulum-associated degradation system. PMID: 25385046
Data indicate that protein tyrosine phosphatase 1B (PTP1B) association with calnexin is ubiquitin conjugating enzyme 9 (UBC9)-dependent. PMID: 25586181
H-ERG trafficking was impaired by H2O2 after 48 h treatment, accompanied by reciprocal changes of expression between miR-17-5p seed miRNAs and several chaperones (Hsp70, Hsc70, CANX, and Golga20) PMID: 24386440
Nef regulates the activity of calnexin to stimulate its interaction with gp160 at the expense of ABCA1 PMID: 25170080
The impact of CANX genetic variants to heart rate was discovered. PMID: 24680774
Soluble calnexin may fulfill functions similar to calreticulin. PMID: 24056258
calnexin tunes the cellular responses to epidermal growth factor receptor in a manner that depends on the health status of the endoplasmic reticulum PMID: 23932718
These findings demonstrated that calnexin strictly monitors the maturation of S protein by its direct binding, resulting in conferring infectivity on severe acute respiratory syndrome coronavirus. PMID: 22915798
The data suggest that patients with low or defective TAP1 or calnexin in primary breast cancers may be at higher risks for developing brain metastasis due to the defects in T cell-based immunosurveillance. PMID: 22065046
cysteine residues within calnexin are important for the structure and function of calnexin. PMID: 21842374
The findings showed that calnexin is a stable component of the ribosome-translocon complex in a manner that is exquisitely dependent on calnexin's palmitoylation status. PMID: 22314232
HLA-I, TAP1, CNX, LMP7, Erp57, Tapasin and ERAP1 were down-regulated in 68%, 44%, 48%, 40%, 52%, 32% and 20% of esophageal squamous cell carcinoma lesions then, respectively. PMID: 21362330
Nixin/ZNRF4 to be central for the regulation of calnexin turnover. PMID: 21205830
Results reveal the P-domain functions as a unique protein-protein interaction domain and implicate a peptidyl prolyl isomerase as a new element in the calnexin cycle. PMID: 20801878
calnexin could bind PrP both in vitro and in vivo; calnexin prevents caspase-3-mediated cytotoxicity induced by PrP PMID: 20506117
Transmembrane segments prevent surface expression of sodium channel Nav1.8 and promote calnexin-dependent channel degradation PMID: 20720009
human delta opioid receptor (hdeltaOR) exists in a ternary complex with SERCA2b and the ER molecular chaperone calnexin PMID: 20528919
Env interacts with intracellular CNX and extracellular PDI via discrete, largely nonoverlapping, regions. PMID: 20202930
Results show that the phosphorylation of calnexin is linked to the efficiency of secretion of the cargo glycoprotein, in this case, alpha1-antitrypsin. PMID: 19815548
Data show that demonstrate that Cnx preferentially associates with misfolded mutant opsins associated with retinitis pigmentosa. PMID: 19801547
EDEM appeared to function in the ERAD (endoplasmic reticulum-associated degradation)pathway by accepting substrates from calnexin. PMID: 12610305
EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle PMID: 12610306
Data show that calnexin associates with newly synthesized proteolipid protein (PLP) molecules, binding stably to misfolded PLP. PMID: 12805210
calnexin-downregulation may contribute to the metastatic phenotype of melanoma cells in vivo PMID: 14732231
Calnexin provides long-term protection of wild-type Shaker protein from ER-associated degradation. PMID: 15161937
the contribution of both the b and b' domains to the binding with CNX and calreticulin was revealed PMID: 15236594
results support emerging models for a glycan-independent chaperone role for calnexin and for the mechanism of retention of misfolded membrane proteins in the endoplasmic reticulum PMID: 15537650
Calnexin decreases with aging and might contribute to a cytoprotection in a variety of human age-related diseases. PMID: 15557823
Data show that the major degradation pathway of the cystic fibrosis transmembrane conductance regulator with F508 deletion from the endoplasmic reticulum is independent of calnexin. PMID: 15923638
Calnexin associates with the neonatal Fc receptor for IgG (FcRn) heavy chain before it associates noncovalently with beta 2-microglobulin. PMID: 16002696
Polypeptide substrate recognition by CANX requires specific conformations of the CANX protein. PMID: 16061483
We characterized a molecular mechanism by which calnexin regulates ER-stress-mediated apoptosis in a manner independent of its chaperone functions but dependent of its binding to Bap31. PMID: 16858427
Here, we have observed that NCT N-linked oligosaccharides mediated specific interactions with the secretory pathway lectins calnexin and ERGIC-53 PMID: 16938437
proportion of the human and the rat WT gonadotropin-releasing hormone receptor appears to be retained in the endoplasmic reticulum by calnexin, an effect that decreases GnRHR signaling capacity PMID: 17170088
These results suggest that MCF-7 resistance to endoplasmic reticulum stress-induced apoptosis is partially mediated by the expression level of calnexin which in turn controls its sub-cellular localization, and its association with Bap31. PMID: 17203246
D1 and D2 dopamine receptor expression is regulated by direct interaction with the chaperone protein calnexin PMID: 17395585
Endoplasmic reticulum chaperones stabilize nicotinic receptor subunits and regulate receptor assembly. PMID: 17728248
Interaction with calnexin led to accumulation of GAT1 in concentric bodies corresponding to previously described multilamellar ER-derived structures. PMID: 18367207
the phosphorylation state of the calnexin cytosolic domain and its interaction with PACS-2 sort the chaperone between domains of the ER and the plasma membrane PMID: 18417615
A dependence on calnexin for proper assembly of CFTR's membrane spanning domains, was identified. PMID: 18716059
ERp57 must be physically associated with the calnexin cycle to catalyze isomerization reactions with most of its substrates PMID: 19054761
alphaIIb interacts with calnexin via its N15-linked glycan, and alphaIIbbeta3 biogenesis is partially controlled by engagement of alphaIIb in the calnexin cycle. PMID: 16304048

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Melanosome.

Protein Families

Calreticulin family

Database Links

HGNC: 1473

OMIM: 114217

KEGG: hsa:821

STRING: 9606.ENSP00000247461

UniGene: Hs.567968

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Code	CSB-RA593154A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg) All lanes: Calnexin antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 68 kDa Observed band size: 94 kDa Exposure time：10s Immunofluorescence staining of Hela cell with CSB-RA593154A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA593154A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CANX Recombinant Monoclonal Antibody

Product Details

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Target Background