Code | CSB-RA228590A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:2000 |
FC | 1:50-1:200 |
CUSABIO's strategy for generating a recombinant monoclonal antibody targeting CAV1 commenced with the immunization of a rabbit using a synthesized peptide from human CAV1. B cells were subsequently isolated from the immunized rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was employed as a template to extend CAV1 antibody genes using degenerate primers. These extended CAV1 antibody genes were then introduced into a plasmid vector and transfected into host cells for expression. The CAV1 recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and subjected to ELISA, WB, and FC applications. It displays specific reactivity with human CAV1 protein.
CAV1 is a structural protein that oligomerizes to form caveolin complexes, which are essential for shaping and stabilizing caveolae on the cell membrane. Caveolae and CAV1 are implicated in lipid transport and metabolism, particularly in regulating lipid droplet formation, lipid uptake, and cholesterol trafficking. CAV1 has been associated with various cellular responses to stress, including oxidative stress, mechanical stress, and cellular damage. It can play a protective role in some instances.
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