CCNB2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA093733A0HU
Size	US$210
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Image	IHC image of CSB-RA093733A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA093733A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O95067

Target Names

CCNB2

Alternative Names

G2/mitotic-specific cyclin-B2, CCNB2

Species Reactivity

Human

Immunogen

A synthesized peptide from human CCNB2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cyclin B2 plays a central role in cell cycle progression, functioning as a regulatory subunit of cyclin-dependent kinases during the G2/M transition. As a key component of the mitotic machinery, CCNB2 accumulates during S and G2 phases before being rapidly degraded at the metaphase-anaphase transition, making it an important marker for studying cell proliferation, mitotic entry, and checkpoint regulation in both normal and malignant cells.

This recombinant monoclonal antibody offers the reproducibility and consistency that demanding research applications require. Generated through recombinant expression technology and derived from a single clone, it provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, combined with affinity chromatography purification, ensures high purity and reliable performance across experiments.

Validation studies demonstrate this antibody's utility across multiple experimental platforms. Immunohistochemistry performed on paraffin-embedded human colorectal cancer tissue shows clear detection of CCNB2 at dilutions between 1:50 and 1:200, with successful staining achieved using standard citrate buffer antigen retrieval. Flow cytometry analysis using HeLa cells confirms intracellular detection capability, with distinct positive signal separation from isotype control, supporting its application in cell cycle studies using this widely-used human cervical carcinoma model.

The validated performance in both tissue sections and cell-based assays makes this antibody well-suited for researchers investigating mitotic regulation, cancer cell proliferation, and cell cycle dynamics. Its compatibility with fixed and permeabilized samples provides flexibility for characterizing CCNB2 expression patterns in human tumor specimens and cultured cell populations.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Gene References into Functions

Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
Data show that the cyclin B2 was overexpressed in bladder cancer tissue. PMID: 26706119
overexpression of CCNB2 protein is associated with clinical progression and poor prognosis in non-small cell lung cancer PMID: 26349989
Cyclin B1 and cyclin B2 are interchangeable for ability to promote G2 and M transition in HeLa cells. PMID: 24324638
there was no significance in the 5-year overall survival rates among the patients with squamous cell carcinoma between expressing lower and higher level of cyclin B2 mRNA PMID: 24375198
Suggest that cytoplasmic CCNB2 may function as an oncogene and could serve as a potential biomarker of unfavorable prognosis over short-term follow-up in breast cancer. PMID: 23282137
The relative expression level of circulating CCNB2 mRNA in cancer patients was significantly higher than that in normal controls and benign diseases group. Circulating CCNB2 mRNA level significantly correlated with cancer stage and metastasis status. PMID: 21161946
Data suggest that cyclin A2 helps initiate mitosis, in part through its effects on cyclin B1, and that cyclins B1 and B2 are particularly critical for the maintenance of the mitotic state. PMID: 20660152
Overexpression of B-type cyclins alters chromosomal segregation PMID: 11960377
p300 binds to multiple NF-Y trimers to regulate cyclin B2 promoter function PMID: 12482752
cyclin B2 expression in colorectal adenocarcinoma is dependent on NF-Y PMID: 17289878
severe reduction by separate RNA interference of either cyclin B1 or cyclin B2 protein levels results in little or no alteration of the cell cycle and, more specifically, of mitosis progression PMID: 17533373
This gene may be used as a very reliable biomarker of lung adenocarcinoma. PMID: 17549666

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Protein Families

Cyclin family, Cyclin AB subfamily

Database Links

HGNC: 1580

OMIM: 602755

KEGG: hsa:9133

STRING: 9606.ENSP00000288207

UniGene: Hs.194698

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Related pathways

p53 signaling pathway Cell cycle Cellular senescence FoxO signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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