CCNB2 Antibody

Datasheet

Code	CSB-PA004808LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate All lanes: CCNB2 antibody at 2.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa Immunofluorescence staining of Hela cells with CSB-PA004808LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CCNB2 Polyclonal antibody

Uniprot No.

O95067

Target Names

CCNB2

Alternative Names

ccnb2 antibody; CCNB2_HUMAN antibody; CycB2 antibody; Cyclin B2 antibody; G2 mitotic specific cyclin B2 antibody; G2/mitotic specific cyclin B2 antibody; G2/mitotic-specific cyclin-B2 antibody; HsT17299 antibody; MGC108931 antibody; MGC140694 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human G2/mitotic-specific cyclin-B2 protein (7-163AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CCNB2 Antibody (Product code: CSB-PA004808LA01HU) is Non-conjugated. For CCNB2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA004808LB01HU	CCNB2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA004808LC01HU	CCNB2 Antibody, FITC conjugated
Biotin	CSB-PA004808LD01HU	CCNB2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

Gene References into Functions

Data show that Islet-1 (ISL1) activated the expression of cyclin B1 (CCNB1), cyclin B2 (CCNB2) and c-myc (c-MYC) genes by binding to the conserved binding sites on their promoters or enhancers. PMID: 27183908
Data show that the cyclin B2 was overexpressed in bladder cancer tissue. PMID: 26706119
overexpression of CCNB2 protein is associated with clinical progression and poor prognosis in non-small cell lung cancer PMID: 26349989
Cyclin B1 and cyclin B2 are interchangeable for ability to promote G2 and M transition in HeLa cells. PMID: 24324638
there was no significance in the 5-year overall survival rates among the patients with squamous cell carcinoma between expressing lower and higher level of cyclin B2 mRNA PMID: 24375198
Suggest that cytoplasmic CCNB2 may function as an oncogene and could serve as a potential biomarker of unfavorable prognosis over short-term follow-up in breast cancer. PMID: 23282137
The relative expression level of circulating CCNB2 mRNA in cancer patients was significantly higher than that in normal controls and benign diseases group. Circulating CCNB2 mRNA level significantly correlated with cancer stage and metastasis status. PMID: 21161946
Data suggest that cyclin A2 helps initiate mitosis, in part through its effects on cyclin B1, and that cyclins B1 and B2 are particularly critical for the maintenance of the mitotic state. PMID: 20660152
Overexpression of B-type cyclins alters chromosomal segregation PMID: 11960377
p300 binds to multiple NF-Y trimers to regulate cyclin B2 promoter function PMID: 12482752
cyclin B2 expression in colorectal adenocarcinoma is dependent on NF-Y PMID: 17289878
severe reduction by separate RNA interference of either cyclin B1 or cyclin B2 protein levels results in little or no alteration of the cell cycle and, more specifically, of mitosis progression PMID: 17533373
This gene may be used as a very reliable biomarker of lung adenocarcinoma. PMID: 17549666

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Protein Families

Cyclin family, Cyclin AB subfamily

Database Links

HGNC: 1580

OMIM: 602755

KEGG: hsa:9133

STRING: 9606.ENSP00000288207

UniGene: Hs.194698

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Protein Service

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Related pathways

p53 signaling pathway Cell cycle Cellular senescence FoxO signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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