Code | CSB-RA204558A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:20-1:200 |
The process for producing a CCNE1 recombinant monoclonal antibody involves four key steps. First, the CCNE1 monoclonal antibody gene is sequenced. Then, the gene is cloned into a plasmid vector and introduced into a host cell line. The CCNE1 recombinant monoclonal antibody is subsequently purified from the cell culture supernatant using affinity chromatography. Finally, the purified antibody is tested and characterized. The CCNE1 monoclonal antibody is created using a synthesized peptide derived from human CCNE1 as the immunogen. This CCNE1 recombinant monoclonal antibody is highly recommended for use in ELISA and IF applications to detect human CCNE1 protein.
The CCNE1 protein mainly regulates the progression of cells through the G1/S checkpoint of the cell cycle. CCNE1 forms a complex with cyclin-dependent kinase 2 (CDK2), which phosphorylates and activates substrates that are involved in DNA replication and cell cycle progression. CCNE1 is expressed in a variety of tissues and is critical for normal cell growth and proliferation. Dysregulation of CCNE1 expression or activity has been associated with various diseases, including cancer.
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