Code | CSB-RA968740A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
The CCNE1 recombinant antibody production process involves four key steps: sequencing the CCNE1 monoclonal antibody gene, inserting the gene into a plasmid vector, transfecting the recombinant vector into a host cell line, and purifying the CCNE1 recombinant monoclonal antibody from the cell culture supernatant via affinity chromatography. The CCNE1 monoclonal antibody is derived from CCNE1 antibody-producing hybridomas, and the production process involves using a synthesized peptide from human CCNE1 as the immunogen. This CCNE1 recombinant monoclonal antibody is recommended for use in detecting human CCNE1 protein through ELISA, IHC, and IF applications.
The CCNE1 protein plays a critical role in the regulation of the cell cycle. It is a regulatory protein that binds to and activates cyclin-dependent kinase 2 (CDK2) to form a complex that is required for the progression of cells from the G1 to the S phase of the cell cycle. This complex phosphorylates various substrates, leading to DNA replication and cell division. The expression of CCNE1 is tightly regulated, with elevated levels of expression associated with various types of cancer. In addition to its role in the cell cycle, CCNE1 has also been implicated in DNA repair and apoptosis.
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