This CCNE1 protein is a semi-custom product. There are 5 expression system options: Yeast, E. coli, In Vivo Biotinylation in E. coli, Baculovirus, and Mammalian cell. Your requirements will be given top priority in determining the protein tags. For proteins within 800 aa, risk-free custom service is guaranteed. It means you will not be charged if the protein cannot be delivered.
CCNE1, a protein crucial for cell cycle regulation, is involved in cell cycle progression, particularly in the G1/S transition by controlling DNA synthesis initiation and other functions during the S phase [1]. It forms a functional complex with CDK2 to regulate the transition from the G1 phase to the S phase [2]. Overexpression of CCNE1 promotes cell cycle progression and tumorigenesis in various cancers, including breast cancer and hepatocellular carcinoma [3][4][5]. Additionally, CCNE1 has been implicated in promoting chromosome instability, DNA replication initiation, and centrosome duplication, which can lead to oncogenesis through genomic instability [6][7].
Studies have shown that CCNE1 is essential for cell cycle re-entry from the G0 phase and oncogenic transformation [5]. Furthermore, CCNE1 has been linked to CDK4/6 inhibitor resistance in breast cancer, where its stabilization by long non-coding RNA (lncRNA) EILA contributes to resistance mechanisms [8]. The stability of cyclin E1 is regulated by various factors, such as NF90 and SPOP, which impact its function in cell cycle progression and cancer development [4][9]. Moreover, the deubiquitinating enzyme OTUB1 has been found to promote prostate cancer progression by stabilizing cyclin E1 [10].
References:
[1] X. Guo and R. Hartley, Hur contributes to cyclin e1 deregulation in mcf-7 breast cancer cells, Cancer Research, vol. 66, no. 16, p. 7948-7956, 2006. https://doi.org/10.1158/0008-5472.can-05-4362
[2] J. Xu, F. Huang, Z. Yao, C. Jia, X. Zhang, H. Lianget al., Inhibition of cyclin e1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression, Cell Communication and Signaling, vol. 17, no. 1, 2019. https://doi.org/10.1186/s12964-019-0398-3
[3] C. Caldon, C. Sergio, J. Kang, A. Muthukaruppan, M. Boersma, A. Stoneet al., Cyclin e2 overexpression is associated with endocrine resistance but not insensitivity to cdk2 inhibition in human breast cancer cells, Molecular Cancer Therapeutics, vol. 11, no. 7, p. 1488-1499, 2012. https://doi.org/10.1158/1535-7163.mct-11-0963
[4] W. Jiang, H. Huang, L. Ding, P. Zhu, H. Saiyin, G. Jiet al., Regulation of cell cycle of hepatocellular carcinoma by nf90 through modulation of cyclin e1 mrna stability, Oncogene, vol. 34, no. 34, p. 4460-4470, 2014. https://doi.org/10.1038/onc.2014.373
[5] X. Guo, Y. Wu, & R. Hartley, Cold‐inducible rna‐binding protein contributes to human antigen r and cyclin e1 deregulation in breast cancer, Molecular Carcinogenesis, vol. 49, no. 2, p. 130-140, 2009. https://doi.org/10.1002/mc.20582
[6] C. Caldon, C. Sergio, J. Schütte, M. Boersma, R. Sutherland, J. Carrollet al., Estrogen regulation of cyclin e2 requires cyclin d1 but not c-myc, Molecular and Cellular Biology, vol. 29, no. 17, p. 4623-4639, 2009. https://doi.org/10.1128/mcb.00269-09
[7] K. Aziz, J. Limzerwala, I. Sturmlechner, E. Hurley, C. Zhang, K. Jeganathanet al., Ccne1 overexpression causes chromosome instability in liver cells and liver tumor development in mice, Gastroenterology, vol. 157, no. 1, p. 210-226.e12, 2019. https://doi.org/10.1053/j.gastro.2019.03.016
[8] Z. Cai, Lncrna eila promotes cdk4/6 inhibitor resistance in breast cancer by stabilizing cyclin e1 protein, Science Advances, vol. 9, no. 40, 2023. https://doi.org/10.1126/sciadv.adi3821
[9] L. Ju, Y. Zhu, Q. Long, X. Li, X. Lin, S. Tanget al., Spop suppresses prostate cancer through regulation of cyclin e1 stability, Cell Death and Differentiation, vol. 26, no. 6, p. 1156-1168, 2018. https://doi.org/10.1038/s41418-018-0198-0
[10] Y. Liao, N. Wu, K. Wang, M. Wang, J. Gao, B. Zhonget al., Otub1 promotes progression and proliferation of prostate cancer via deubiquitinating and stabling cyclin e1, Frontiers in Cell and Developmental Biology, vol. 8, 2021. https://doi.org/10.3389/fcell.2020.617758
