CCNE2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA045623A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: U-251MG whole cell lysate(30µg), A431 whole cell lysate(30µg), Raji whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: Cyclin E2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time：2min IHC image of CSB-RA045623A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA045623A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA045623A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O96020

Target Names

CCNE2

Alternative Names

G1/S-specific cyclin-E2, CCNE2

Species Reactivity

Human

Immunogen

A synthesized peptide from human CCNE2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14F8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cyclin E2 plays a central role in cell cycle progression, functioning as a regulatory subunit of CDK2 to drive the G1/S phase transition. As a key component of the cell cycle machinery, CCNE2 is frequently dysregulated in various cancers, making it an important marker for studies investigating proliferation, tumorigenesis, and cell cycle checkpoint control.

This recombinant monoclonal antibody, generated from clone 14F8, offers the consistency and reliability that demanding research applications require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, you can expect reproducible performance across experiments and between lots—an essential consideration for longitudinal studies or when establishing standardized protocols in your laboratory.

Validation testing demonstrates robust performance across multiple experimental platforms. Western blot analysis detects CCNE2 at the expected 47 kDa molecular weight in human cell lines including U-251MG glioblastoma, A431 epidermoid carcinoma, Raji B lymphocytes, and THP-1 monocytic cells, confirming reliable detection across diverse cellular contexts. Immunohistochemistry staining has been validated in paraffin-embedded human placenta tissue using citrate buffer antigen retrieval, while immunofluorescence studies in HeLa cells reveal the expected nuclear and cytoplasmic localization pattern consistent with cyclin E2's biological function. Flow cytometry validation further extends the utility of this antibody for quantitative single-cell analysis of CCNE2 expression levels.

This antibody serves researchers investigating cell cycle regulation, cancer biology, and proliferative disorders. Its validated performance across immunoblotting, tissue staining, cellular imaging, and flow cytometric applications provides flexibility for comprehensive characterization of CCNE2 expression in your experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential for the control of the cell cycle at the late G1 and early S phase.

Gene References into Functions

the molecular mechanism of trastuzumab action in BT474 cell line may be regulated by miR-26a and miR-30b and CCNE2 overexpression might play an important role in acquired trastuzumab resistance in HER2+ breast cancer. PMID: 28120942
Knockdown of CCNE2 promoted MCF7 cell apoptosis and G2-stage cell cycle arrest. PMID: 30157476
Combinatorial PX-866 and Raloxifene Decrease Rb Phosphorylation, Cyclin E2 Transcription, and Proliferation of MCF-7 Breast Cancer Cells PMID: 26660119
The results suggest that miR30a may function as a novel tumor suppressor in CRPC. Its antioncogenic activity may occur by the reduced expression of a distinct cell cycle protein, CCNE2. PMID: 27431942
Overexpression of cyclin E2 is an early event in gastric carcinogenesis. PMID: 27034264
Survivin and cyclin E2 genes expression may have clinical relevance and can be considered as molecular risk factors for AL. Also they may be useful as predictive markers for treatment outcome in leukemic patients. PMID: 26600399
miR-26a regulated mouse hepatocyte proliferation by directly targeting the 3' untranslated regions of cyclin D2/cyclin E2. PMID: 26818545
In breast cancer patients, high levels of HMGA1 and CCNE2 expression are associated with the YAP/TAZ signature. PMID: 26265440
data suggests the potential role of monomorphic morphology, high cyclin E2 and Ki67 expression as adverse prognostic factors for TNKLPD PMID: 25475054
miR-144-5p functions as tumour suppressor in BC cells. CCNE1 and CCNE2 were directly regulated by miR-144-5p and might be good prognostic markers for survival of bladder cancer patients PMID: 26057453
Our study shows miR-25 is overexpressed in small cell lung cancer and acting as oncogenic regulator by regulating cyclin E2. PMID: 25550809
Results suggest that the miR-30d-5p/CCNE2 axis may contribute to NSCLC cell proliferation and motility. PMID: 25843294
Data indicate that miR-26a overexpression inhibited pancreatic cancer cell growth by the downregulation of cyclin E2 expression. PMID: 24116110
In cancer cells Fbw7, fails to effectively target cyclin E2 for proteosomal degradation. PMID: 23324394
Cyclin E2 induction of genomic instability by a mechanism distinct from cyclin E1 indicates that these two proteins have unique functions in a cancer setting. PMID: 23324395
protein and mRNA expressions of Cyclin E2 in nasopharyngeal carcinoma PMID: 22394640
Tamoxifen resistant tumors displayed enriched expression of genes related to cell cycle and proliferation, as well as elevated activity of E2F transcription factors. PMID: 21789246
expression is deregulated in primary breast tumors PMID: 12466974
The CCNE2 qualify as independent prognostic markers for lymph node-negative breast cancer patients. PMID: 16740753
Since SCF(Fbxw7/hCdc4) is functionally inactivated in several cancer types, alteration of this molecular pathway could contribute to the deregulation of cyclin E2 in tumorigenesis. PMID: 19084516
A hypothetical model whereby PTEN loss upregulates cell cycle genes such as cdc6 and cyclin E2 that in turn promote metastatic colonization at distant sites. PMID: 19107233
Data indicate that cyclin E2-Cdk2 activation by estrogen occurs via E2F- and CHD8-mediated transcription of cyclin E2 downstream of cyclin D1, and does not require c-myc. PMID: 19564413
Investigated downregulation of coronary vascular smooth muscle cell growth by siRNA against E2F1, cyclins E1 and E2. Data shows reduction in the phosphorylation levels of the retinoblastoma protein pRB and a decrease in the amount of cyclin A2. PMID: 19603101
Loss of cyclin D1 in ovarian cancer cells treated with SHetA2 is sufficient to induce G(1) cell cycle arrest and this strategy is not impeded by the presence of cyclin E2. PMID: 19638577

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Subcellular Location

Nucleus.

Protein Families

Cyclin family, Cyclin E subfamily

Tissue Specificity

According to PubMed:9858585, highest levels of expression in adult testis, thymus and brain. Lower levels in placenta, spleen and colon. Consistently elevated levels in tumor-derived cells compared to non-transformed proliferating cells. According to PubM

Database Links

HGNC: 1590

OMIM: 603775

KEGG: hsa:9134

STRING: 9606.ENSP00000309181

UniGene: Hs.521693

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

p53 signaling pathway PI3K-Akt signaling pathway Cell cycle Cellular senescence

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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