CCT2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA242230A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg),Raji whole cell lysate(30µg), Hela whole cell lysate(30µg), SW620 whole cell lysate(30µg),Mouse brain tissue lysate(30µg),Rat brain tissue lysate(30µg) All lanes: TCP 1 beta antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time：1min Overlay Peak curve showing Raji cells stained with CSB-RA242230A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P78371

Target Names

CCT2

Alternative Names

99D8.1 antibody; CCT 2 antibody; CCT beta antibody; CCT-beta antibody; CCT2 antibody; CCTB antibody; Chaperonin containing t complex polypeptide 1 beta subunit antibody; Chaperonin containing t complex polypeptide 1 subunit 2 antibody; Chaperonin containing TCP1 subunit 2 antibody; Chaperonin containing TCP1 subunit 2 (beta) antibody; CTP:phosphocholine cytidylyltransferase 2 antibody; Epididymis secretory sperm binding protein Li 100n antibody; HEL S 100n antibody; MGC142074 antibody; MGC142076 antibody; MGC94480 antibody; PRO1633 antibody; T complex protein 1 beta subunit antibody; T complex protein 1 subunit beta antibody; T-complex protein 1 subunit beta antibody; TCP 1 beta antibody; TCP-1-beta antibody; TCPB_HUMAN antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human CCT2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.

Gene References into Functions

the novel LCA mutations in CCTbeta and the impact of chaperon disability by these mutations in cellular biology. PMID: 27645772
A role for the TRiC subunits TCP1 and CCT2, and potentially the entire TRiC complex, in breast cancer. PMID: 25704758
Increased expression of CCT2 is associated with tumor progression and the clinical behavior of gallbladder carcinoma. PMID: 23782473
PDCD5 bound the apical domain of the CCTbeta subunit, projecting above the folding cavity without entering it. Like PDCD5, beta-tubulin also interacts with the CCTbeta apical domain, but a second site is found at the sensor loop deep within the folding cavity. PMID: 24375412
PB2 associates with CCT2 as a monomer and the CCT binding site is located in a central region of the PB2 protein. PMID: 20573828
The chaperonin CCT is identified as a novel physiological substrate for p90 ribosomal S6 kinase (RSK) and p70 ribosomal S6 kinase (S6K). PMID: 19332537

Hide All

Subcellular Location

Cytoplasm.

Protein Families

TCP-1 chaperonin family

Database Links

HGNC: 1615

OMIM: 605139

KEGG: hsa:10576

STRING: 9606.ENSP00000299300

UniGene: Hs.189772

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address