Product Details

Uniprot No.

Target Names

Alternative Names

CD74; DHLAG; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; CD antigen CD74

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8H11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CD74, also known as the invariant chain or Ii, serves as a critical chaperone for MHC class II molecules, guiding their assembly and trafficking within antigen-presenting cells. Beyond this classical role, CD74 functions as a receptor for macrophage migration inhibitory factor (MIF), positioning it at the intersection of antigen presentation and inflammatory signaling. This dual functionality makes CD74 a compelling target for researchers investigating immune regulation, autoimmune disorders, and tumor immunology, where its expression patterns often correlate with disease progression.

This recombinant monoclonal antibody, clone 8H11, offers the reproducibility that demanding immunological studies require. Because the antibody sequence is defined and produced through recombinant expression in rabbit host cells, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations.

Validation testing demonstrates reliable performance across complementary platforms. Immunohistochemical staining in paraffin-embedded human tissues, including ovarian cancer and endometrial samples, shows specific detection at 1:100 dilution using citrate buffer antigen retrieval, giving you confidence when examining CD74 expression in archival clinical specimens. Flow cytometry analysis using Raji cells, a B-cell lymphoma line with robust MHC class II expression, confirms the antibody's utility for quantitative single-cell studies. This combination of tissue-based and cell-based validation provides flexibility whether your workflow centers on spatial expression patterns or population-level analysis.

For researchers exploring antigen presentation mechanisms, tumor microenvironment characterization, or MIF-mediated signaling pathways, this antibody delivers the specificity and consistency needed to generate reliable, publishable data in human immunology applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.; Binds to the peptide-binding site of MHC class II alpha/beta heterodimers forming an alpha-beta-CLIP complex, thereby preventing the loading of antigenic peptides to the MHC class II complex until its release by HLA-DM in the endosome.; Stabilizes the conformation of mature CTSL by binding to its active site and serving as a chaperone to help maintain a pool of mature enzyme in endocytic compartments and extracellular space of antigen-presenting cells (APCs). Has antiviral activity by stymieing the endosomal entry of Ebola virus and coronaviruses, including SARS-CoV-2. Disrupts cathepsin-mediated Ebola virus glycoprotein processing, which prevents viral fusion and entry. This antiviral activity is specific to p41 isoform.

Gene References into Functions

High CD74 expression is associated with progressive multiple sclerosis. PMID: 28923927
These findings predict the binding mode of Hu-MIF-1 and orthologs with CD74. PMID: 28513076
results suggest that two immune biomarkers, CD74 and IL10, could be relevant tools for the identification of Intensive care unit-acquired infections risk in Intensive care unit patients. PMID: 28477143
Increased CD74 expression levels predicted worse stroke severity and outcomes in subjects with ischemic stroke. PMID: 27884769
The data validate CD74 as a useful prognostic tumor cell protein marker associated with favorable recurrence-free survival and overall survival in stage III melanoma. PMID: 26783288
These results have implications for the manner in which D-DT and MIF compete with each other for binding to the CD74 receptor and for the relative potency of DRa1-MOG-35-55 and RTL1000 for competitive inhibition of D-DT and MIF binding and activation through CD74. PMID: 27573366
CLIP expression and activated gamma-delta T cells are responsible for the development of preeclampsia. PMID: 28642294
these observations are consistent with the view that CD74 expression in tumor cells promotes the intratumoral immune response and is associated with a better prognosis in basal-like breast cancer PMID: 27058619
MIF-CD74 signaling inhibits interferon (IFN)-gamma secretion in microglia through phosphorylation of microglial ERK1/2 (extracellular signal-regulated protein kinases 1 and 2). The inhibition of MIF signaling or its receptor CD74 promotes IFN-gamma release and amplifies tumor death either through pharmacological inhibition or through siRNA-mediated knockdown. PMID: 27157615
High CD74 expression is associated with colorectal cancer. PMID: 27599658
the roles of CD74 and MIF in the immune surveillance escape process PMID: 26866879
CD74 downregulation in placental macrophages is present in preeclampsia. PMID: 27199465
CD74 was increased in burn patients. PMID: 27209369
predicted binding of one CD74 trimer to a single RTL1000 antagonist utilized the same two 5 residue determinants PMID: 26851955
an inverse correlation with the tumor size for stromal MIF and a positive correlation with the triple receptor negative tumor status for stromal CD74 seem to be showed PMID: 26412712
Studies indicate that the invariant chain (CD74) is critical for antigen presentation. PMID: 27033518
FOXP1 represents a novel regulator of genes targeted by the class II MHC transactivator CIITA and CD74. PMID: 26500140
Interferon gamma promotes melanoma cell survival regulating CD74-MIF interaction. PMID: 26039541
The results of this stusy suggested that the CD74 represents a positive prognostic marker most probably because of its association with an M1-polarized immune milieu in high-grade gliomas. PMID: 25175718
Invariant chain is a new chaperone for TLR7 in B cells. (Review) PMID: 26198699
CD74 expression and its therapeutic potential in thyroid carcinoma PMID: 25600560
Specific targeting of the CD74 on the lymphoma cell surface will sensitize CD74-expressing cancer cells to Fas-mediated apoptosis. PMID: 25304249
A conserved WW domain-like motif regulates CD74 antigen-dependent cell-surface transport of the NKG2D ligand ULBP2. PMID: 25983110
these results demonstrate that the subunit stoichiometry of oligomeric Ii/MHCII complexes is influenced by p35. PMID: 24638068
Findings showed an increased extent of MIF expression in cancer cells and in stromal fibroblasts of Breast Cancer tumor, in contrast to a less uniform increase of CD74 expression. PMID: 24939415
In human B cells, SPPL2a is indispensable for turnover of CD74 N-terminal fragment. PMID: 25035924
Results show that CD74-NRG1 gene fusions are activating genomic alterations in invasive mucinous lung adenocarcinomas. PMID: 24469108
In TPA-induced skin inflammation, MIF is released from damaged keratinocytes and then triggers the chemotaxis of CD74(+)CXCR2(+) NKT cells for IFN-gamma production. PMID: 25172501
Data show that clear cell renal cell carcinoma (ccRCC) tissue and malignant cell lines expressed higher levels of CD74 and hypoxia inducible factor 1alpha (HIF-1alpha) than adjacent normal renal tissue and normal cell HK-2. PMID: 23273913
Polymorphisms in CD74 is associated with hematologic toxicity in patients with non-small-cell lung cancer after platinum-based chemotherapy. PMID: 24220096
MIF/CD74 pathway may represent a crucial target for treating disc degeneration since inhibiting the function of MIF with its antagonist ISO-1 can reduce MIF-induced inflammation and exert potent therapeutic effects. PMID: 24569872
demonstrate that CLIP expression on leukemia-associated phenotype (LAP)-positive cells during follow-up is significantly correlated with a shortened relapse-free survival PMID: 24731748
High CD74 expression is associated with head and neck squamous cell carcinomas. PMID: 24663824
High expression of CD74 is an independent prognostic factor for prolonged overall survival in mesothelioma patients PMID: 24594996
These results demonstrate natural antagonist activity of DRalpha1 for macrophage migration inhibitory factor PMID: 24683185
Recombinant vaccinia virus vaccines encoding CD74 may be useful tools to improve CD4 T-cell responses to viral and tumour antigens. PMID: 24205828
when CD74 is overexpressed in human cancer and noncancerous epithelial cells, it interacts and interferes with the function of Scribble PMID: 23730214
Evaluated expression of CD74 in chronic lymphocytic leukemia patients. CD74 expression was significantly higher in CLL group than in controls. There was positive correlation between CD74 and ZAP70 expression. PMID: 23572149
Our data do not replicate prior reports of LN-2 as a sensitive and specific marker for undifferentiated pleomorphic sarcoma PMID: 23000905
CD74 antigen expression is increased in high grade, invasive urothelial bladder cancer. PMID: 22905972
Ii regulates the repertoire of tumor peptides presented by major histocompatibility complex class II+ breast cancer cells. PMID: 22942358
Data show that human Iip35 isoform (CD74 antigen) is expressed in mouse antigen presenting cells. PMID: 22689013
Data indicate that 25 +/- 1.3% of CD74 and 17 +/- 0.3% of HLA-DR are colocalized. PMID: 22889831
a novel regulatory mechanism governing cell migration during intervertebral disc degeneration PMID: 22952837
High Cd74 expression is associated with lymph node metastasis and triple-negative breast cancer. PMID: 22935920
Hepatitis C virus-mediated inhibition of cathepsin S increases invariant-chain expression on hepatocyte surface. PMID: 22761382
Expression of CD74-ROS in noninvasive NSCLC cell lines readily conferred invasive properties that paralleled the acquisition of E-Syt1 phosphorylation. PMID: 22659450
Stat1 and CD74 overexpression is co-dependent and linked to increased invasion and lymph node metastasis in triple-negative breast cancer. PMID: 22178447
Upregulation of MIF, CD74 and TLR4 are associated with increasing clinical stage and provide an opportunity as novel gastric cancer chemoprevention and/or treatment strategy. PMID: 22611320
This is the first study reporting that Ii chain up-regulation occurs on naturally infected antigen presenting cells obtained directly from HIV(+) subjects. PMID: 21945129

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Involvement in disease

A chromosomal aberration involving CD74 is found in a non-small cell lung tumor. Results in the formation of a CD74-ROS1 chimeric protein.

Subcellular Location

Cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane. Golgi apparatus, trans-Golgi network. Endosome. Lysosome. Note=Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.; [Isoform p41]: Late endosome. Lysosome.

Tissue Specificity

[Isoform p41]: In B cells, represents 10% of total CD74 expression.; [Isoform p33]: In B cells, represents 70% of total CD74 expression.

Database Links

HGNC: 1697

OMIM: 142790

KEGG: hsa:972

STRING: 9606.ENSP00000009530

UniGene: Hs.436568

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Code	CSB-RA004956A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA004956A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-RA004956A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Overlay histogram showing Raji cells stained with CSB-RA004956A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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CD74 Recombinant Monoclonal Antibody

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