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The CLDN6/9 recombinant monoclonal antibody was meticulously produced by CUSABIO following a well-defined process. B cells were isolated from the spleen of an immunized animal, with the recombinant human CLDN6/9 protein used as the immunogen during the immunization procedure. RNA was extracted from the B cells and reverse-transcribed into cDNA. The gene encoding the CLDN6/9 antibody was then extended using a degenerate primer and inserted into a vector. This recombinant vector was subsequently introduced into host cells through transfection, enabling efficient antibody expression. The CLDN6/9 recombinant monoclonal antibodies were harvested from the cell culture supernatant and purified using affinity chromatography. Extensive validation, including ELISA and FC testings, was conducted to confirm this antibody's reactivity with human CLDN6 and CLDN9 protein, ensuring its accuracy and suitability for further applications.
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