CLDN6/9 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA005508MA3HU
Size	US$210
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Image	The Binding Activity of Human CLDN6 with Anti-CLDN6-9 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6 (CSB-MP005508HU(A4)) at 10 μg/mL can bind Anti-CLDN6-9 recombinant antibody. The EC₅₀ is 1.189-1.618 ng/mL. The VLPs (CSB-MP3838) is negative control. The Binding Activity of Human CLDN6 with Anti-CLDN6-9 recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN6-9 (CSB-MP005511HU) at 10 μg/mL can bind Anti-CLDN6-9 recombinant antibody. The EC₅₀ is 2.876-3.657 ng/mL. The VLPs (CSB-MP3838) is negative control. Untransfected HEK-293T cells (green line) and transfected Human CLDN6 HEK-293T stable cells (red line) were stained with anti-CLDN6/9 antibody(CSB-RA005508MA3HU) (2µg/110⁶cells), washed and then followed by APC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry. Untransfected CHO-K110⁶cells), washed and then followed by APC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry. Untransfected HEK-293T cells (green line) and transfected Human CLDN9 HEK-293T stable cells (red line) were stained with anti-CLDN6/9 antibody (CSB-RA005508MA3HU) (2µg/1*10⁶cells), washed and then followed by APC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry. The Binding Activity of Mouse cldn6 with Anti-CLDN6/9 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse cldn6(CSB-MP3347MO) at 5 μg/mL can bind Anti-CLDN6/9 recombinant antibody. The EC₅₀ is 7.349-12.12 ng/mL.
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Target Background

Product Details

Uniprot No.

P56747

Target Names

CLDN6/9

Alternative Names

Claudin-6 antibody; Claudin6 antibody; CLD6_HUMAN antibody; CLDN 6 antibody; CLDN6 antibody; OTTHUMP00000159248 antibody; Skullin 2 antibody; Skullin antibody; UNQ757/PRO1488 antibody

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human CLDN6/9 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

mFc2a

Clone No.

9E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Claudin-6 and Claudin-9 are closely related tight junction proteins that play essential roles in epithelial barrier formation and cell-cell adhesion. CLDN6 has garnered significant attention in cancer research due to its restricted expression pattern—largely silenced in normal adult tissues but aberrantly reactivated in various malignancies, making it an attractive target for therapeutic development and tumor biology studies. Both claudins also contribute to developmental processes and cellular signaling pathways, positioning them as valuable targets for researchers investigating stem cell biology, tissue morphogenesis, and signal transduction mechanisms.

This recombinant monoclonal antibody, clone 9E2, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing researchers to confidently compare results across extended studies without concerns about antibody variability affecting their data.

Validation studies demonstrate robust performance in both ELISA and flow cytometry applications. Functional ELISA testing confirms high-affinity binding to human CLDN6 with EC50 values in the low nanogram per milliliter range, while VLP negative controls verify binding specificity. Flow cytometry validation using CLDN6-transfected and CLDN9-transfected HEK-293T stable cell lines shows clear discrimination between transfected and untransfected populations, confirming the antibody's utility for detecting both claudin family members in cell-based assays. Cross-species reactivity extends to mouse CLDN6, as demonstrated by ELISA binding studies, providing flexibility for researchers working with murine model systems.

This antibody serves investigators exploring developmental biology, microbiology, signal transduction, and stem cell research, offering a reliable tool for characterizing claudin expression and function across multiple experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a major role in tight junction-specific obliteration of the intercellular space.; (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Gene References into Functions

Antibodies recognizing native CLDN6 as displayed on cell surfaces and mediating complement-dependent cytotoxicity were elicited in vaccinated animals. The data suggest applications of CLDN6 displaying Virus-like particles in cancer immunotherapy. PMID: 29131519
these results suggest that Helicobacter pylori lipopolysaccharide induces TLR2 expression in the gastric adenocarcinoma cells, and that the longer the exposure to lipopolysaccharide, the greater the expression of TLR2 in the cell membrane; consequently the expression of claudin-4, -6, -7 and -9 also increases PMID: 29031421
Study provides evidence that high expression of CLDN6 confers chemoresistance on breast cancer which is mediated by GSTP1, the activity of which is regulated by p53. PMID: 29116019
CLDN6 enhances the chemoresistance to ADM via activating the AF-6/ERK signaling pathway and up-regulating cancer stem cell characters in MDAMB231 cells. PMID: 29159771
we demonstrated that the downregulation of CLDN6 is regulated through promoter methylation by DNMT1, which depends on the SMAD2 pathway, and that CLDN6 is a key regulator in the SMAD2/DNMT1/CLDN6 pathway to inhibit EMT, migration and invasion of breast cancer cells PMID: 28867761
In conclusion, this information from bioinformatics analysis will help future attempts to better understand CLDN6 regulation and functions. PMID: 28656265
high expression of CLDN 6 was observed in approx. 65% of the myxofibrosarcomas, whereas the benign soft tissue tumors did not show a high expression of CLDN 6. The expression of CLDN 6 in the myxofibrosarcomas was significantly higher than those of other tumor specimens. Among the myxofibrosarcomas, the high expression of CLDN 6 was correlated with high FNCLCC grades and high AJCC stages. PMID: 28476380
Results show that DNA methylation down-regulates CLDN6 expression through MeCP2 binding to the CLDN6 promoter, deacetylating H3 and H4, and altering chromatin structure, consequently promoting migratory and invasive phenotype in breast cancer cells. PMID: 27461117
Cldn6 was decreased in alveolar type II-like epithelial cells (A549) and primary small airway epithelial cells when exposed to cigarette smoke ext PMID: 27982694
suggest that claudin-6 induces MMP-2 activation through claudin-1 membrane expression PMID: 27914788
Data show that claudin-6 (CLDN6) R209Q and occludin (OCLN) P24A mutations do not affect HCV pseudoparticles (HCVpp) entry. PMID: 26561856
The expression of ASK1 is correlated with the level of claudin-6 in cervical carcinoma cells and tissues. PMID: 26191261
High levels of CLDN6 are associated with non-small-cell lung cancer. PMID: 24710653
The expression of claudin-6 was down regulated in gastric cancer tissue. PMID: 23919729
Only some hepatitis C virus strains efficiently use CLDN6 for infection. PMID: 23775920
This work provides a proof of concept for the use of Claudin-6 to eliminate residual undifferentiated human pluripotent stem cells from culture. PMID: 23778593
Although claudin-6 and claudin-9 can serve as entry factors in cell lines, hepatitis C virus infection into human hepatocytes is not dependent on claudin-6 and claudin-9. PMID: 23864633
ASK1 signal may play a positive role in the inhibitory effect of claudin-6 in breast cancer. PMID: 22925655
Our results show that claudin-6 protein is significantly down-regulated in breast invasive ductal carcinomas PMID: 22455563
CLDN6 is not a specific biomarker for atypical teratoid rhabdoid tumors as it is expressed in a variety of other pediatric CNS and soft tissue tumors. PMID: 21989342
17beta-E2 might regulate the expression of claudin-6 and inhibit the proliferation and migration of MCF-7 cells. PMID: 20388399
Increased expression of claudin-6, claudin-7, or claudin-9 is sufficient to enhance tumorigenic properties of a gastric adenocarcinoma cell line. PMID: 20874001
CLDN6 may be a useful positive marker to help further identify atypical teratoid/rhabdoid tumors for diagnostic and treatment purposes PMID: 19220299
Claudins 6, 7, and 9 expressions are closely related to gastric carcinogenesis PMID: 19960275
The up-regulation of claudin-6 expression in MCF-7 breast cancer cells suppresses their malignant phenotypes with a correlation with the restoration of tight junction integrity. PMID: 20367941
claudin-6, downregulates the malignant phenotype of breast carcinoma. PMID: 20215972
Claudin 6 was not found in epithelioid glioblastomas or rhabdoid glioblastomas. PMID: 20118769
CLDN6 and CLDN9, but not CLDN1, are expressed in peripheral blood mononuclear cells, an additional site of HCV replication. PMID: 17804490
claudin-6 and claudin-9 expressed in CD81+ cells also enable the entry of HCV pseudoparticles derived from six of the major genotypes. PMID: 18234789
CLDN6, clustered with CLDN9 at human chromosome 16p13.3, is a four-transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C. PMID: 12736707

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Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

Protein Families

Claudin family

Tissue Specificity

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

Database Links

HGNC: 2048

OMIM: 615798

KEGG: hsa:9074

STRING: 9606.ENSP00000328674

UniGene: Hs.533779

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