Product Details

Uniprot No.

Target Names

DGAT1

Alternative Names

ACAT related gene product 1 antibody; ACAT-related gene product 1 antibody; Acyl coenzyme A:cholesterol acyltransferase related gene 1 antibody; Acyl-CoA retinol O-fatty-acyltransferase antibody; Acyl-CoA:diacylglycerol acyltransferase antibody; ARAT antibody; ARGP1 antibody; C75990 antibody; D15Ertd23e antibody; Dgat antibody; DGAT1 antibody; DGAT1_HUMAN antibody; Diacylglycerol O acyltransferase 1 antibody; Diacylglycerol O-acyltransferase 1 antibody; DIAR7 antibody; Diglyceride acyltransferase antibody; EC 2.3.1.20 antibody; hCG_24006 antibody; MGC139064 antibody; Retinol O fatty acyltransferase antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human DGAT1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Diacylglycerol O-acyltransferase 1 (DGAT1) catalyzes the final committed step in triglyceride biosynthesis, converting diacylglycerol and fatty acyl-CoA into triacylglycerol. This enzyme plays a central role in lipid metabolism, energy storage, and intestinal fat absorption, making it a compelling target for researchers investigating metabolic disorders, obesity, and lipid-related signaling pathways. DGAT1 also functions as an acyl-CoA retinol O-fatty-acyltransferase, linking it to retinoid metabolism and expanding its relevance across multiple research domains.

This recombinant monoclonal antibody, clone 14A8, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your results remain comparable across extended studies and collaborative projects.

Validation data demonstrates reliable performance across multiple applications. In western blot analysis, the antibody detects DGAT1 at the expected 55 kDa molecular weight in human cell lysates, including COLO205, HepG2, and A431 lines, with recommended dilutions ranging from 1:500 to 1:5000. Flow cytometry validation using MCF-7 cells confirms the antibody's utility for intracellular protein detection, with clear separation between specific staining and isotype control populations at 1:100 dilution. The antibody is also validated for ELISA applications.

Raised in rabbit against a synthetic peptide derived from human DGAT1, this affinity-purified IgG arrives in a glycerol-containing buffer optimized for long-term storage stability. Whether you are exploring lipid metabolism, investigating metabolic disease mechanisms, or characterizing signal transduction pathways, this antibody provides a dependable tool for your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. In contrast to DGAT2 it is not essential for survival. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG).

Gene References into Functions

Study identified a large cohort of patients with congenital diarrheal disorders with mutations in DGAT1 that reduced expression of its product; dermal fibroblasts and intestinal organoids derived from these patients had altered lipid metabolism and were susceptible to lipid-induced cell death. Expression of full-length wildtype DGAT1 or DGAT2 restored normal lipid metabolism in these cells. PMID: 29604290
our findings indicate that inhibition of both DGAT1 and ABHD5 using siRNA leads to reduction in prostate cancer cell growth. PMID: 28877685
data reveal an important role for DGAT activity and TG synthesis generally in averting ER stress and lipotoxicity, with specifically DGAT1 performing this function during stimulated lipolysis in adipocytes. PMID: 28768178
DGAT1 mutations result in a spectrum of diseases PMID: 28373485
A novel homozygous missense variant was identified in a family with protein losing enteropathy. A splice site mutation in intron 8 was identified in a second family with PLE. These cases of DGAT1 deficiency extend the molecular and phenotypic spectrum of PLE. PMID: 26883093
DGAT1 expression is down-regulated in viral hepatitis-related cirrhosis. PMID: 26493024
Data indicate no clinically relevant pharmacokinetic interaction between DGAT-1 inhibitor pradigastat and rosuvastatin. PMID: 25740267
the apparent lack of therapeutic window owing to GI side effects of AZD7687, particularly diarrhoea, makes the utility of DGAT1 inhibition as a novel treatment for diabetes and obesity questionable. PMID: 24118885
Downregulation of CLDN1 was associated with altered fatty acid homeostasis in the absence of DGAT1. PMID: 24899196
MGAT2 functions as a dimeric or tetrameric protein and selectively heterodimerizes with DGAT1 in mammalian cells PMID: 24573674
The structure-activity relationship studies of a novel series of carboxylic acid derivatives of pyridine-carboxamides as DGAT-1 inhibitors is described. PMID: 23317570
adipose overexpression of Dgat1 gene in transgenic mice leads to diet-inducible insulin resistance. PMID: 16306352
Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with the viral capsid core PMID: 23420847
results identify DGAT1 loss-of-function mutations as a rare cause of congenital diarrheal disorders PMID: 23114594
describe distinct but synergistic roles of the two DGATs in an integrated pathway of TAG synthesis and secretion, with DGAT2 acting upstream of DGAT1 PMID: 22748069
a comprehensive evaluation of a small molecule inhibitor for DGAT1 and suggests that pharmacological inhibition of DGAT1 holds promise in treating diverse metabolic disorders. PMID: 21990351
DGAT1 belongs to the family of oligomeric membrane proteins that adopt a dual membrane topology. PMID: 21846726
human small intestinal DGAT, which is mainly encoded by DGAT1, utilizes 1,2-DAG as the substrate to form TAG PMID: 21369919
The triglyceride-synthesizing enzyme diacylglycerol acyltransferase-1 (DGAT1) is identified as a key host factor for hepatitis C virus infection. PMID: 20935628
Identify DGAT1 as an important protein that participates in the effect of HNF4A on hepatic secretion of triglyceride-rich lipoproteins. PMID: 20167659
Polymorphism associated with alterations in body mass index, high density lipoprotein levels and blood pressure in Turkish women. PMID: 12123490
DGAT1 overexpression in murine white adipose tissue provides a model in which obesity does not impair glucose disposal PMID: 12401709
DGAT participates in the regulation of membrane lipid synthesis and lipid signaling, thereby playing an important role in modulating cell growth properties PMID: 14557275
Niacin selectively inhibited DGAT2 but not DGAT1 activity, but had no effect on the expression of DGAT1 and DGAT2 mRNA PMID: 15258194
thiazolidinediones upregulate the adipocyte lipid storage genes DGAT and FAS but have no significant effect on LPL PMID: 16894240
Review summarizes current knowledge of DGAT1 and DGAT2 enzymes, focusing on new advances since the cloning of their genes, including possible roles in human health and diseases. PMID: 18757836
the acylation of acylglycerols by DGAT1 is important for dietary fat absorption in the intestine PMID: 18768481
Report visceral and subcutaneous adipose tissue diacylglycerol acyltransferase activity in humans. PMID: 19197254

Hide All

Involvement in disease

Diarrhea 7 (DIAR7)

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily

Database Links

HGNC: 2843

OMIM: 604900

KEGG: hsa:8694

STRING: 9606.ENSP00000332258

UniGene: Hs.521954

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Code	CSB-RA044388A0HU
Size	US$210
	Order now
Image	Western Blot , Positive WB detected in:COLO205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: DGAT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time：10s Overlay Peak curve showing MCF-7 cells stained with CSB-RA044388A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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DGAT1 Recombinant Monoclonal Antibody

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Target Background