Product Details

Uniprot No.

Target Names

Alternative Names

0610041G12Rik antibody; DBLOH_HUMAN antibody; DBOH antibody; DFNA64 antibody; diablo antibody; Diablo homolog (Drosophila) antibody; Diablo homolog antibody; Diablo homolog mitochondrial antibody; Diablo IAP binding mitochondrial protein antibody; Diablo like protein antibody; DIABLO S antibody; Direct IAP binding protein with low pI antibody; Direct IAP-binding protein with low pI antibody; FLJ10537 antibody; FLJ25049 antibody; mitochondrial antibody; Mitochondrial Smac protein antibody; Second mitochondria derived activator of caspase antibody; Second mitochondria-derived activator of caspase antibody; second mitochondrial activator of caspases antibody; SMAC 3 antibody; Smac antibody; Smac protein antibody; SMAC3 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human DIABLO

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14H5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DIABLO, also known as Smac (second mitochondria-derived activator of caspase), plays a pivotal role in the intrinsic apoptotic pathway by antagonizing inhibitor of apoptosis proteins (IAPs). Upon mitochondrial outer membrane permeabilization, DIABLO is released into the cytosol where it binds and neutralizes IAPs, thereby promoting caspase activation and programmed cell death. This regulatory function makes DIABLO a significant target in cancer research, particularly in studies investigating therapeutic strategies to overcome apoptosis resistance in tumor cells.

This recombinant rabbit monoclonal antibody (clone 14H5) offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human DIABLO, the recombinant format ensures sequence-defined specificity and eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, providing confidence across longitudinal studies and collaborative projects.

Validation across multiple platforms demonstrates versatile experimental utility. Western blot analysis confirms robust detection in diverse human cell lines including HEK293, PC-3, SW620, Jurkat, MCF-7, A431, and HepG2, with cross-species reactivity verified in NIH/3T3 cells and mouse heart tissue. The observed band at 21 kDa, rather than the predicted 27 kDa, reflects the mature processed form of DIABLO following mitochondrial import signal cleavage. Immunohistochemical staining in human glioma tissue sections reveals clear target localization, while flow cytometry analysis in SH-SY5Y neuroblastoma cells demonstrates suitability for single-cell quantification studies.

Whether investigating mitochondrial apoptotic signaling, screening for therapeutic compounds that modulate IAP function, or characterizing DIABLO expression in cancer models, this antibody provides a reliable tool for advancing your apoptosis research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Gene References into Functions

Mechanistic studies showed that Smac can inhibit the expression of Survivin, promote cell apoptosis of drug-resistant ovarian cancer cells and reverse the drug resistance. PMID: 29562492
Serum Smac expression level was significantly lower in the EAOC group than in the control group and benign ovarian tumor group (P< 0.05), while HE4 and CA125 expression levels were significantly higher in the EAOC group than the other two groups. PMID: 29226858
SMAC expression in locally advanced breast cancer is a novel favourable prognostic factor in LABC for pathological complete remission and disease-free survival. PMID: 29895124
administration of SMAC or BH3 mimetics following short-term paclitaxel treatment could be an effective therapeutic strategy for TNBC, while only BH3-mimetics could effectively overcome long-term paclitaxel resistance. PMID: 28187446
Antagonism strategies to modulate the actions of XIAP, cIAP1/2 and survivin are the central focus of current research and this review highlights advances within this field with particular emphasis upon the development and specificity of second mitochondria-derived activator of caspase (SMAC) mimetics (synthetic analogs of endogenously expressed inhibitors of IAPs SMAC/DIABLO). PMID: 28424988
analysis of Smac-mediated apoptosis in chronic lymphocytic leukemia cells PMID: 27223062
Data show that oncolytic viruses (OV) and second mitochondrial activator of caspase (Smac)-mimetic compounds (SMC) synergistically kill cancer cells directly. PMID: 28839138
Expressions of SDF-1, survivin and smac were significantly higher in epithelial ovarian cancer tissue than those in normal tissue. PMID: 28852723
Results indicate that Smac plays an important role in reticulum stress-induced apoptosis in human lens epithelial cells, suggesting its close association with cataract development. PMID: 28682901
Smac mimetic APG-1387 exerts a potent antitumor effect on nasopharyngeal carcinoma cells by inducing apoptosis. PMID: 27424523
Study found a negative correlation between Smac and XIAP at the level of protein but not mRNA in non-small cell lung carcinoma (NSCLC) patients. Overexpressed XIAP could degrade through ubiquitination, the mature Smac inhibiting NSCLC apoptosis. PMID: 27498621
Report role of RIP1 in Smac mimetic mediated chemosensitization of neuroblastoma cells. PMID: 26575016
This review discusses the promise as well as some challenges at the translational interface of exploiting Smac mimetics as cancer therapeutics. PMID: 26567362
Data indicate that Smac/DIABLO showed an inverse correlation with inhibitor of apoptosis proteins XIAP, cIAP-1 and cIAP-2. PMID: 25549803
Data show that mitochondrial X-linked inhibitor of apoptosis protein (XIAP) entry requires apoptosis regulatory proteins Bax or Bak through mitochondrial permeabilization and Smac/DIABLO protein degradation. PMID: 26134559
SapC-DOPS acts through a mitochondria-mediated pathway accompanied by an early release of Smac and Bax. PMID: 25889084
this is the first demonstration that a dual approach using simultaneous overexpression of a cell penetrable form of Smac and TRAIL sensitize and promote apoptotic process even in resistant breast cancer cells. PMID: 25586349
Preoperative measurement of serum VEGF, survivin, and Smac/DIABLO may be of help in early detection of serous ovarian cancer and may provide important information about the patient's outcome and prognosis. PMID: 25577253
Smac-DIABLO adopts a tetrameric assembly in solution. PMID: 25650938
IRF1 is a dual regulator of BV6-induced apoptosis and inflammatory cytokine secretion. PMID: 25501823
CLIC4, ERp29, and Smac/DIABLO integrated into a novel panel based on cancer stem-like cells in association with metastasis stratify the prognostic risks of colorectal cancer. PMID: 24916695
Within mitochondria, XIAP selectively signals lysosome- and proteasome-associated degradation of its inhibitor Smac. PMID: 25080938
Describe a new alternatively spliced isoform of Smac which promotes thr formation of mammospheres. PMID: 25337193
The phosphorylation of Smac at the Nterminal serine 6 residue is functionally linked to Smac release during TNFalphainduced apoptosis. PMID: 25310587
XIAP, cIAP1, and cIAP2, members of inhibitor of apoptosis (IAP) proteins, are critical regulators of cell death and survival; the SMAC/DIABLO protein is an endogenous antagonist of XIAP, cIAP1, and cIAP2 PMID: 24841289
It represents a powerful way to enhance the destruction of cancer cells and increase the efficiency and duration of gene expression required for apoptosis. PMID: 24771354
Over-expression of cellular Smac can inhibit inhibitor of apoptosis proteins (IAPs), enhance caspases activity and the apoptosis rate of PC-3 cells induced by TRAIL, which may provide a useful experimental basis for prostate cancer therapy. PMID: 22528226
The present study indicated the significance of Smac and survivin in determining the breast cancer response to anthracyclinebased chemotherapy, and may permit further stratifying of prechemotherapy patients to undertake more tailored treatments. PMID: 24317109
Smac/DIABLO decreases the proliferation and increases the apoptosis of hypertrophic scar fibroblasts. PMID: 23857156
Results show that Smac mimetics exerts an antitumor effect on nasopharyngeal carcinoma cancer stem cells. PMID: 23699656
We report that an Smac-mimetic selectively induces TNF-alpha-dependent cystic renal epithelial cell death, leading to the removal of cystic epithelial cells from renal tissues and delaying cyst formation. PMID: 23990677
The activin A signals via SMAD proteins, but not TAK1 or p38, to regulate murine and ovine Fshb transcription in gonadotrope-like cells. PMID: 22549017
Results demonstrate an essential and apoptosis-independent function of SMAC in tumor suppression and provide new insights into the biology and targeting of colon cancer. PMID: 22751125
Overexpression of Smac promotes Cisplatin-induced apoptosis by activating caspase-3 and caspase-9 in lung cancer. PMID: 23252748
Expressions of SMAC/DIABLO and survivin were significantly reciprocal in breast cancer and benign tumor tissues. PMID: 22161156
Identification of a novel anti-apoptotic E3 ubiquitin ligase that ubiquitinates antagonists of inhibitor of apoptosis proteins SMAC, HtrA2, and ARTS. PMID: 23479728
Smac, XIAP, caspase 3 might be associated with the growth and carcinogenesis of nonnasal inverted papilloma. PMID: 23156805
Higher expression of Smac and Ki-67 appear to play a role in the pathogenesis of pancreatic cancer. Combined detection of these proteins may improve the prognostic evaluation of this disease. PMID: 22534537
differential redistribution of cyt c and Smac occurs under various conditions PMID: 22848756
these data suggest a new mechanism by which NOXA chemosensitized ovarian cancer cells to cisplatin by inducing alterations in the Bax/Smac axis. PMID: 22590594
Overall, the findings suggest that measuring the levels of Smac/DIABLO in the serum may be considered a prognostic parameter in patients with bladder cancer. PMID: 22218530
Data show that the apoptosis rate of Eca109/Smac was significantly increased with the concentration of cispaltin increased. PMID: 22482401
The over-expression of PTEN gene may inhibit the proliferation of K562 cells and promote cell apoptosis via the regulation of Survivin, Xiap and Smac genes. PMID: 22333553
Data show that Smac mimetic- and TNFalpha-mediated cell death occurs without characteristic features of apoptosis (i.e., caspase activation, DNA fragmentation) in FADD-deficient cells. PMID: 22028622
Data suggest that downregulation of Smac may be a chemoresistance mechanism in ESCC. PMID: 21676925
Results establish a critical role of Smac in mediating therapeutic responses of HNSCC cells and provide a strong rationale for combining Smac mimetics with other anticancer agents to treat HNSCC. PMID: 21242120
Low Smac expression is associated with breast cancer. PMID: 21744997
DFNA64 genotype is the human genetic disorder associated with DIABLO malfunction and suggests that mutant DIABLO(S71L) might cause mitochondrial dysfunction. PMID: 21722859
Patients with positive smac/DIABLO tumors had a longer disease-specific survival when compared with those with negative tumors in the 10-year follow-up. PMID: 21478115
The dimerization of Smac is critical for the XIAP-mediated retention of Smac at or inside the mitochondria. PMID: 21354220

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Involvement in disease

Deafness, autosomal dominant, 64 (DFNA64)

Subcellular Location

Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.

Tissue Specificity

Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.

Database Links

HGNC: 21528

OMIM: 605219

KEGG: hsa:56616

STRING: 9606.ENSP00000398495

UniGene: Hs.169611

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Code	CSB-RA586177A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg),PC-3 whole cell lysate(30µg),SW620 whole cell lysate(30µg),Jurkat whole cell lysate(30µg),MCF-7 whole cell lysate(30µg),A431 whole cell lysate(30µg),HepG2 whole cell lysate(30µg),NIH/3T3 whole cell lysate(30µg),Mouse heart tissue lysate(30µg) All lanes: DIABLO antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 21 kDa Exposure time：20s IHC image of CSB-RA586177A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA586177A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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DIABLO Recombinant Monoclonal Antibody

Product Details

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Target Background