DLX5 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA948135A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), Hela whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Raji whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: DLX5 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time：2min IHC image of CSB-RA948135A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA948135A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA948135A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P56178

Target Names

DLX5

Alternative Names

Homeobox protein DLX-5, DLX5

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human DLX5 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21F4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DLX5 is a homeobox transcription factor that plays essential roles in craniofacial development, osteogenesis, and neuronal differentiation. As a key regulator of bone formation, DLX5 drives osteoblast maturation and skeletal patterning, making it a critical target for researchers investigating developmental biology, bone disorders, and cancer progression where aberrant DLX5 expression has been implicated.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide from human DLX5, offers the consistency and reliability that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant technology, you can expect reproducible performance across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations.

Validation across multiple platforms demonstrates this antibody's versatility in your research toolkit. Western blot analysis confirms specific detection in diverse cell lines including K562, HeLa, HEK293T, Raji, and NIH/3T3 lysates, with the observed band at approximately 35 kDa slightly above the predicted 32 kDa molecular weight. This modest shift likely reflects post-translational modifications common to transcription factors. Immunohistochemistry staining has been validated in paraffin-embedded human cervical cancer and testis tissues using citrate buffer antigen retrieval, revealing clear nuclear localization patterns consistent with DLX5's function as a transcription factor. Flow cytometry applications have been confirmed using 786-O renal carcinoma cells, enabling quantitative analysis of DLX5 expression at the single-cell level.

With cross-species reactivity for human and mouse samples, this antibody supports both clinical specimen analysis and model organism studies. Researchers investigating skeletal development, stem cell differentiation, or tumor biology will find this antibody well-suited for their mechanistic and translational studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.

Gene References into Functions

Our results in mice suggest that long-range DLX5 enhancer elements located in the human SLC25A13 gene may underlie the sensorineural hearing loss that is sometimes associated with SHFM1. PMID: 29301908
These results indicate activation of DLX5 and RUNX2 via its distal promoter represents a unique feature of GFs, and is important for ECM regulation. Down-regulation of these transcription factors in PAFs could be associated with their property to degrade collagen, which may impact on the process of periodontitis. PMID: 27645561
these findings indicate that, in MSCs, DLX5 is a master regulator of osteogenesis. Furthermore, tanshinone IIA may be valuable for stem cell-based therapies of certain bone diseases. PMID: 28949384
As a result of disturbed imprinting, the upregulated DLX5 affects trophoblast proliferation in preeclampsia. PMID: 28904069
These data indicate that certain missense mutations diminish the ability of the Dlx5 homeodomain to recognize and bind target DNAs, and they likely destabilize the formation of functional complexes. PMID: 26829219
In cells grown on titanium support, DLX5 and RUNX1 were respectively upregulated (+3.12-fold) and downregulated (-2.14-fold) PMID: 25025858
Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I. PMID: 25332435
Heterozygous DLX5 nonsense mutation c.G115T(p.E39X) associated with isolated split-hand/foot malformation with reduced penetrance and variable expressivity in two unrelated Polish families. PMID: 25196357
A novel heterozygous mutation in exon 3 of DLX5 found in the family members with SHFM1 phenotype. PMID: 24496061
Genome sequencing of the deletion breakpoints showed that the DLX5 and DLX6 genes are disomic but the putative DYNC1I1 exon 15 and 17 enhancers are deleted. PMID: 24459211
The duplicated region harbors only DLX5 and DLX6, which are known for their role in SHFM1. PMID: 23169702
The strongest evidence for altered methylation patterns in shiftworkers was observed for DLX5 gene. PMID: 23193016
Cyclic tensile stress may induce differentiation of periodontal ligament stem cells towards mineralized tissue cells by promoting Dlx5 mRNA expression and decreasing Msx2 expression. PMID: 22332551
Two patients that have in common a p63-Dlx5/Dlx6 pathway dysregulation. PMID: 22342398
the first intragenic DLX5 mutation in split hand and foot malformation is found; a potential dual role for DLX5 in limb development is suggested PMID: 22121204
DLX5 is significantly increased in heart tissue from calcific aortic valve patients compared to controls. PMID: 21205918
MDA-MB-231 breast neoplasms did not express DLX5 but the resulting bone/lung metastases did. PMID: 21108812
Data suggest that DLX5 plays a significant role in the pathogenesis of some ovarian cancers by enhancing IRS-2-AKT signaling. PMID: 21045156
p63 binds to an enhancer element in the SHFM1 locus and this element controls expression of DLX6 and DLX5 which are important for limb development. PMID: 20808887
Results describe the expression of DLX5 and DLX6 in autistic spectrum disorder patients in an attempt to identify potential abnormality of expression. PMID: 19195802
A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. PMID: 11959851
DLX5 was confirmed to be imprinted in normal human lymphoblasts and brain tissues by a polymorphic analysis PMID: 12782124
DLX5 is a target for MeCP2, linking genomic imprinting and Rett syndrome [review] PMID: 15954098
In dental follicle cells, gene expression of runx2, DLX-5, and MSX-2 was unaffected during osteogenic differentiation in vitro. PMID: 16467978
high expression of mutated MECP2 in TRD mutation showed bialleic expression of DLX5 suggesting loss of imprinting PMID: 17363207
DLX5 and DLX6 are not imprinted in humans and are not likely to be direct targets of MeCP2 modulation. PMID: 17701895
Dlx5 can act as an oncogene by cooperating with Akt2 to promote lymphomagenesis PMID: 18316591
Activation of placenta-specific transcription factor distal-less homeobox 5 predicts clinical outcome in primary lung cancer patients. PMID: 18413826
The MYC promoter is specifically activated by overexpression of DLX5. PMID: 19497851
Dlxin-1 binds Dlx5 and several additional homeodomain proteins and may regulate the function of Dlx family members in bone formation PMID: 11084035

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Involvement in disease

Split-hand/foot malformation 1 with sensorineural hearing loss, autosomal recessive (SHFM1D)

Subcellular Location

Nucleus.

Protein Families

Distal-less homeobox family

Database Links

HGNC: 2918

OMIM: 220600

KEGG: hsa:1749

STRING: 9606.ENSP00000222598

UniGene: Hs.99348

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Email: [email protected]
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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Signaling pathways regulating pluripotency of stem cells

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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