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The generation of the DSG2 recombinant monoclonal antibody involved the incorporation of DSG2 antibody genes into plasmid vectors. These engineered plasmid vectors were subsequently introduced into suitable host cells using exogenous protein expression techniques to facilitate antibody production. After that, the DSG2 recombinant monoclonal antibody underwent a purification process via affinity chromatography. Rigorous validation for ELISA suitability of this antibody was performed. In a functional ELISA assay, it was established that the DSG2 recombinant monoclonal antibody exhibited robust binding affinity to the human DSG2 protein (CSB-MP622752HU) at a concentration of 2 μg/mL, with an EC50 falling within the range of 20.26 to 38.00 ng/mL.
The main function of the DSG2 protein is to mediate cell-cell adhesion by forming desmosomes, thereby contributing to tissue integrity and stability in various tissues, including the skin and the heart. Dysregulation of DSG2 can have significant implications for tissue function and may be associated with certain diseases such as pemphigus vulgaris and arrhythmogenic cardiomyopathy.
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