EIF1AX Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA698596A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), A549 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A375 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: eIF1AX antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 18 kDa Exposure time：1min IHC image of CSB-RA698596A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA698596A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA698596A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P47813

Target Names

EIF1AX

Alternative Names

Eukaryotic translation initiation factor 1A, X-chromosomal (eIF-1A X isoform) (Eukaryotic translation initiation factor 4C) (eIF-4C), EIF1AX, EIF1A EIF4C

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Antibody

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EIF1AX plays a fundamental role in the initiation of protein synthesis, functioning as a critical component of the translation machinery that facilitates ribosomal scanning and start codon recognition. This eukaryotic translation initiation factor has garnered significant research attention due to its involvement in various cellular processes and its emerging connections to cancer biology, particularly in uveal melanoma and thyroid carcinomas where EIF1AX mutations have been identified as potential drivers.

This recombinant monoclonal antibody, generated against a synthetic peptide from human EIF1AX, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced through recombinant technology, clone 15E4 eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations, ensuring your results remain comparable across experiments.

Validation data demonstrates robust performance across multiple applications and sample types. Western blot analysis confirms reliable detection in human cell lines including HeLa, A549, HEK293, A375, and HepG2, with the observed band appearing at approximately 18 kDa rather than the predicted 16 kDa—a shift likely attributable to post-translational modifications common to translation factors. Cross-species reactivity extends to mouse liver and rat brain tissue lysates, broadening utility for comparative studies. Immunohistochemistry validation in human ovarian cancer and lung tissue sections demonstrates clear staining patterns suitable for pathological investigations, while flow cytometry data from HeLa cells confirms applicability for single-cell analysis of intracellular EIF1AX expression.

Whether investigating translational control mechanisms, exploring cancer-associated mutations, or characterizing protein synthesis pathways, this antibody provides a dependable tool for researchers working at the intersection of gene expression regulation and disease biology.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.

Gene References into Functions

Its mutation is found in patients with meningeal melanocytic tumor. PMID: 29476293
Study identified a new RNA-induced silencing complex component, eIF1A, which directly interacts with the MID-domain of Ago2 and functions in DICER-independent miRNA biogenesis and miRNA-mediated RNA interference. PMID: 29236257
EIF1AX alterations occurred infrequently in low-grade gliomas (1.4%), uterus endometrial carcinoma (1.25%), thyroid carcinoma (1%), and lung adenocarcinoma (0.4%). PMID: 27089234
In this report we show that patients with uveal melanoma harbor mutation-specific chromosomal patterns in the tumor. These chromosomal patterns are characterized by different types of chromosomal anomalies, thus illustrating that distinct biological mechanisms underlie uveal melanoma pathogenesis. PMID: 27916271
Coexpression of mutant NRAS and EIF1AX proteins promoted proliferation and clonogenic survival in LGSC cells, providing the first example of co-occurring, growth-promoting mutational events in ovarian cancer. PMID: 28646021
Short 5'UTR mRNAs are enriched with TISU (translation initiator of short 5'UTR), a 12-nucleotide element directing efficient scanning-independent translation. This study demonstrate that TISU is particularly dependent on eukaryotic initiation factor 1A (eIF1A) which interacts with both RPS3 and RPS10e. PMID: 28584194
Mutation in EIF1AX gene is associated with Uveal Melanoma. PMID: 28810145
we find iris melanomas to be related genetically to choroidal and ciliary body melanomas, frequently harboring GNAQ, GNA11, and EIF1AX mutations. PMID: 28700778
results indicate that the interactions between eIF1A and eIF5B are being continuously rearranged during translation initiation; presentation of a model how the dynamic eIF1A/eIF5B interaction network can promote remodeling of the translation initiation complexes, and the roles in the process played by intrinsically disordered protein segments PMID: 27325746
Patients with uveal melanoma can be classified into 3 groups, of which EIF1AX-mutated tumors and tumors without BAP1, SF3B1, or EIF1AX mutations are associated with prolonged survival and low metastatic risk, SF3B1-mutated tumors are associated with late metastasis PMID: 26923342
BAP1, SF3B1, and EIF1AX mutations occur during uveal melanoma tumor progression in an almost mutually exclusive manner and are associated with different levels of metastatic risk. PMID: 27123562
We report here the occurrence of EIF1AX mutations not only in thyroid cancer, but also in benign thyroid nodules, and demonstrate that phenotypically these mutations are associated with the encapsulated follicular variant of papillary thyroid carcinoma and benign follicular-pattern nodules. PMID: 26911375
The finding of this study suggested that an SF3B1 or EIF1AX mutation is present in a substantial subset of primary LMNs underscores that these tumors genetically resemble uveal melanoma and are different from cutaneous melanoma at the genetic level. PMID: 26769193
The effect of an EIF1AX mutation is estimated to be a decreased odds of metastasis in uveal melanoma. PMID: 24970262
the newly identified driver mutations in EIF1AX and SF3B1, specifically found in uveal melanomas with disomy 3, confirm and extend the established classification model of uveal melanoma. PMID: 23793026
Coordinated movements of eukaryotic translation initiation factors eIF1, eIF1A, and eIF5 trigger phosphate release from eIF2 in response to start codon recognition by the ribosomal preinitiation complex PMID: 23293029
eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RNA recognition motif and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition PMID: 20060839
Remarkable conservation of translation initiation factors: IF1/eIF1A and IF2/eIF5B are universally distributed phylogenetic markers. PMID: 11699879
Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. PMID: 12426392
determination of binding site on eukaryotic initiation factor 5B PMID: 12569173
The efficiency of the EF-1alpha promoter was higher than that of the cytomegalovirus promoter in all embryonic stem cells trnasfected. PMID: 17784828

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Protein Families

EIF-1A family

Database Links

HGNC: 3250

OMIM: 300186

KEGG: hsa:107984923

STRING: 9606.ENSP00000368927

UniGene: Hs.522590

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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