All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Peptide
All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
  • Peptide

ELK1 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA971574A0HU
Size US$210
Order now
Image
  • Western Blot
    Positive WB detected in: A431 whole cell lysate(30µg), HT-29 whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg)
    All lanes: ELK1 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/40000 dilution
    Predicted band size: 45 kDa
    Observed band size: 62 kDa
    Exposure time：3min
  • Overlay Peak curve showing Hela cells stained with CSB-RA971574A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Uniprot No.
P19419
Target Names
ELK1
Alternative Names
ETS domain-containing protein Elk-1, ELK1
Species Reactivity
Human
Immunogen
A synthesized peptide from human ELK1 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
15D2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
FC 1:50-1:200
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

ELK1 is a member of the ETS transcription factor family that plays a central role in immediate-early gene regulation, functioning as a key nuclear target of the MAPK signaling cascade. Upon phosphorylation by ERK1/2, ELK1 activates transcription of genes involved in cell proliferation, differentiation, and survival, making it an important node for studying signal transduction from growth factor receptors to transcriptional outcomes. Researchers investigating oncogenic signaling, neuronal plasticity, or stress responses frequently examine ELK1 activity as a readout of pathway activation.

This recombinant monoclonal antibody, clone 15D2, offers the reproducibility that demanding experimental workflows require. Because recombinant antibodies are produced from a defined sequence rather than harvested from serum, you can expect consistent performance across lots, eliminating the variability that can complicate longitudinal studies or multi-site collaborations. The rabbit IgG format provides strong signal amplification while maintaining specificity against the immunizing peptide derived from human ELK1.

Validation by western blot demonstrates reliable detection across multiple human cell lines, including A431, HT-29, HeLa, and K562 whole cell lysates at 1:1000 dilution. The observed band at 62 kDa, rather than the predicted 45 kDa molecular weight, likely reflects post-translational modifications such as phosphorylation or glycosylation that are characteristic of transcriptionally active ELK1. Flow cytometry validation in HeLa cells confirms utility for single-cell analysis of intracellular ELK1 levels, expanding your options for quantitative studies.

Whether you are mapping MAPK pathway dynamics, characterizing transcription factor networks, or profiling signaling in cancer cell models, this antibody provides a dependable tool for your research.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

ELK1 Antibodies

ELK1 Proteins

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation.
Gene References into Functions
  1. enhanced ETS factor activity and the transcription of ETS family target genes related to spliceosome function and cell death induction via alternate MCL1 splicing, is reported. PMID: 29118074
  2. Authors found that miR-185-5p decreases HBV S1p activity by targeting ELK1. PMID: 30308183
  3. High ELK1 expression is associated with thyroid cancer progression. PMID: 30015900
  4. ID1, CTCF and ELK1 may be associated with prostate cancer, and may be potential therapeutic targets for the treatment of this disease. PMID: 29956775
  5. Study demonstrates that miR-135a regulates cell proliferation in breast cancer by targeting ELK1 and ELK3 oncogenes, and suggests that miR-135a potentially can act as a tumor suppressor. PMID: 29892795
  6. Phospho-ELK1 overexpression serves as a predictor of poor prognosis in patients with urothelial carcinoma of the upper urinary tract. PMID: 29518027
  7. TAB1 was identified as a functional target of miR-134, and the expression of TAB1 was increased by the transcription factors of NF-kappaB1, c-Rel, and ELK1 via miR-134. PMID: 28206956
  8. This study indicated that lncrna-TCONS_00026907 was upregulated in cervical cancer and TCONS_00026907 promoted the progression of cervical cancer through inhibition of miR-143-5p and promotion of ELK1. PMID: 28544557
  9. FOXE1 interacts with ELK1 on thyroid relevant gene promoters, establishing a new regulatory pathway for its role in adult thyroid function. Co-regulation of TERT suggests a mechanism by which allelic variants in/near FOXE1 are associated with thyroid cancer risk. PMID: 27852061
  10. Data suggest that inability of lithium, an anti-manic agent, to regulate circadian rhythms in cells from patient with bipolar disorder reflects reduced ERK1/2 activity and MAP kinase signaling through ELK1. (ERK = extracellular signal-related kinase; ELK1 = ETS-domain protein ELK1) PMID: 27216486
  11. we have shown that miR-1275 is a novel negative regulator of human visceral preadipocyte differentiation, which appears to act via post-transcriptional silencing of ELK1. PMID: 27154547
  12. Purified ELK1 and AR bound with a dissociation constant of 1.9 x 10(-8) m A purified mutant ELK1 in which the D-box and DEF motifs were disrupted did not bind AR. PMID: 27793987
  13. Study identified ELK1 as a novel target for miR- 150 which is up-regulated in apoptotic human umbilical cord vein endothelial cells. PMID: 28110404
  14. Study identified a core region encompassing nt - 118 to + 108 of IPO4 gene that is necessary for its promoter activity. Transcription factors binding to this region were screened, resulting in the identification of two members of the Ets family, Ets-like transcription factor-1 and GA binding protein, which repress or activate its promoter activity, respectively. PMID: 28254634
  15. High expression of ELK1 is associated with cholangiocarcinoma. PMID: 27658773
  16. Overexpression of PAD4 constrains the activity of EMT via suppressing Elk1 expression. PMID: 27176594
  17. These data reveal a novel role for Elk1 regulating ITGB6 expression and highlight how dysregulation of Elk1 can contribute to human disease. PMID: 26861876
  18. Downregulated expression of transcriptional activator ELK-1 may play an important role in the pathogenesis of atrial fibrillation. PMID: 26617947
  19. ELK1 is likely to be activated in prostate cancer cells and promote tumor progression. Furthermore, silodosin that inactivates ELK1 in prostate cancer cells not only inhibits their growth but also enhances the cytotoxic activity of gemcitabine. PMID: 26864615
  20. results suggest that ELK1 plays an important role in bladder tumorigenesis and cancer progression. PMID: 26342199
  21. Negative feedback regulation of AXL by miR-34a modulates apoptosis in lung cancer cells by activating the transcription factor ELK1 via the JNK signaling pathway. PMID: 26667302
  22. These findings suggest that PKCalpha expression in HCC could be stimulated by the formation of MZF-1/Elk-1 complex, which directly binds to the PKCalpha promoter. PMID: 26010542
  23. interleukin-1beta (IL-1beta)-induced IER3 expression is mediated by the ERK1/2 target, transcription factor Elk-1. PMID: 25066273
  24. Data show that afatinib reduced Elk-1 transcription factor binding to the CIP2A protein promoter and suppressed CIP2A transcription. PMID: 25537503
  25. TNF-alpha modulation of intestinal epithelial tight junction barrier is regulated by ERK1/2 activation of Elk-1. PMID: 24121020
  26. Analysis implies a role of ELK-1 in the differences between pluripotent stem cells with distinct X chromosome inactivation statuses. PMID: 23871667
  27. Elk-1 interacts with the cell cycle kinase Aurora-A, and when Aurora inhibitors are used, P-S383-Elk-1 fails to localize to the poles and remains associated with DNA. PMID: 23322625
  28. our data suggest that preferential binding of activated Elk-1 to the IL10 rs3122605-G allele upregulates IL10 expression and confers increased risk for SLE in European Americans. PMID: 24130510
  29. Two members of the ETS (E-26) family (PEA3 and ELK-1) regulate the expression of miRNA-200b. PEA3 promotes the expression of miRNA-200b, and ELK-1 is a transcriptional repressor of miRNA-200b. PMID: 24072701
  30. FBXO25 mediates ELK-1 degradation through the ubiquitin proteasome system and thereby plays a role in regulating the activation of ELK-1 pathway in response to mitogens PMID: 23940030
  31. The binding of Ets1 and Elk1 together to the proximal CIP2A promoter is absolutely required for CIP2A expression in cervical, endometrial and liver carcinoma cell lines PMID: 23117818
  32. Sorafenib induces endometrial carcinoma apoptosis by inhibiting Elk-1-dependent Mcl-1 transcription and inducing Akt/GSK3beta-dependent protein degradation. PMID: 23463670
  33. Authors define the minimal promoter region of EVI1 and demonstrate that RUNX1 and ELK1, two proteins with essential functions in hematopoiesis, regulate EVI1 in AML. PMID: 22689058
  34. Ethanol increases Pol III transcription through a response element which is composed of the overlapping Elk1 and AP-1 binding sites of the TBP promoter. The binding sites may play a role in ethanol-induced deregulation of Pol III genes in liver tumors. PMID: 23454483
  35. Strikingly, promoters bound by ELK1 without ERK2 are occupied by Polycomb group proteins that repress genes involved in lineage commitment. PMID: 23727019
  36. although ELK1 and GABPA ultimately control the same biological process, they do so by regulating different cohorts of target genes associated with cytoskeletal functions and cell migration contro PMID: 23284628
  37. Elk-1 pT417 is present in epithelial cell nuclei of various normal and cancer tissues and the number of pT417-positive cells correlates with differentiation grade of colonic adenocarcinomas. PMID: 23114923
  38. The ETS domain transcription factor ELK1 directs a critical component of growth signaling by the androgen receptor in prostate cancer cells. PMID: 23426362
  39. Elk1 is positively associated with estrogen receptor and Cyclin D1 expression in breast cancer. Luminal A/B Her-2 negative subtypes showed more Elk-1 activity compared to Her-2 and Basal subtype. No clinicopathologic or prognostic associations were found. PMID: 23127278
  40. This is demonstrated for the unique binding mode where a novel role for ELK1 in controlling cell migration is revealed. PMID: 22589737
  41. demonstrate that ELK-1 expression arises by a combination of leaky scanning and reinitiation, with the latter mediated by the small upstream ORF2 conserved in both spliced isoforms PMID: 22354998
  42. Although the findings showed elevated expression of Elk-1 and PKCalpha in 5637 cells, the regulator of PKCalpha in bladder cancer cells is yet to be determined. PMID: 22559731
  43. association between the expression of PKCalpha and the expression of the transcription factors Elk-1 and MZF-1 in breast cancer cell lines PMID: 22242952
  44. Activation of the Elk-1 led to an increased survival and a proliferative response with the EGF stimulation, and knocking-down the Elk-1 caused a decrease in survival of U138 glioblastoma cells. PMID: 22085529
  45. Formation of ternary complex of human biliverdin reductase-protein kinase Cdelta-ERK2 protein is essential for ERK2-mediated activation of Elk1 protein, nuclear factor-kappaB, and inducible nitric-oxidase synthase (iNOS). PMID: 22065579
  46. DJ-1 regulates SOD1 expression through Erk1/2-Elk1 pathway in its protective response to oxidative insult. PMID: 21796667
  47. the ERK/ELK-1 cascade is involved in p53-independent induction of p21 and BAX gene expression. PMID: 21642427
  48. Genome-wide analysis reveals PADI4 cooperates with Elk-1 to activate c-Fos expression in breast cancer cells. PMID: 21655091
  49. AC3-33 is a novel member of the secretory family and inhibits Elk1 transcriptional activity via ERK1/2 MAP PMID: 20680465
  50. preferential activation of PTPRZ1 by HIF-2 results at least in part from cooperative binding of HIF-2 and ELK1 to nearby sites on the PTPRZ1 promoter region PMID: 20224786

Show More

Hide All

Subcellular Location
Nucleus.
Protein Families
ETS family
Tissue Specificity
Lung and testis.
Database Links

HGNC: 3321

OMIM: 311040

KEGG: hsa:2002

STRING: 9606.ENSP00000247161

UniGene: Hs.181128

Promotions
Reward For Reviews
Free antibody trial
Contact Us

  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Related Articles
Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?
Related pathways
ErbB signaling pathway MAPK signaling pathway Focal adhesion MAPK Erk pathway Ras signaling pathway Insulin signaling pathway
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of email
Email
[email protected]
icon of address
Address
No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2026 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Privacy Policy
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*