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GOLM1 Recombinant Monoclonal Antibody

Datasheet
Code CSB-RA009666A0HU
Size US$210
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Image
  • Western Blot
    Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate
    All lanes: GOLM1 antibody at 1.3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46, 45 KDa
    Observed band size: 80 KDa
  • Overlay histogram showing Hela cells stained with CSB-RA009666A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q8NBJ4
Target Names
GOLM1
Alternative Names
bA379P1.3 antibody; C9orf155 antibody; Chromosome 9 open reading frame 155 antibody; Golgi membrane protein 1 antibody; Golgi membrane protein GP73 antibody; Golgi phosphoprotein 2 antibody; Golgi protein 73 kD antibody; Golgi protein 73kD antibody; GOLM 1 antibody; GOLM1 antibody; GOLM1_HUMAN antibody; GOLPH 2 antibody; GOLPH2 antibody; GP 73 antibody; GP73 antibody; PSEC0257 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide derived from human GOLM1
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
9C2
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, WB, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Protocols
ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

GOLM1, also known as GP73 or Golgi phosphoprotein 2, is a type II Golgi membrane protein that has garnered significant attention in signal transduction research and as a potential biomarker in liver pathology. This protein is notably upregulated in hepatocellular carcinoma and various liver diseases, making it a valuable target for researchers investigating disease mechanisms, diagnostic marker development, and Golgi apparatus biology.

This recombinant monoclonal antibody, clone 9C2, offers the reproducibility and consistency that demanding experimental workflows require. Because it is produced from a defined sequence rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format and affinity-chromatography purification ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, this antibody successfully detects GOLM1 in both SH-SY5Y neuroblastoma and HepG2 hepatocellular carcinoma whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 80 kDa, higher than the predicted 45-46 kDa molecular weight, reflects the extensive glycosylation characteristic of this Golgi-resident protein. Flow cytometry validation in HeLa cells confirms reliable intracellular detection following appropriate fixation and permeabilization, with clear signal separation from control conditions.

Whether you are exploring GOLM1's role in hepatic disease progression, investigating Golgi membrane dynamics, or developing diagnostic assays, this antibody provides the validated performance and recombinant consistency needed to advance your research with confidence.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Customer Reviews and Q&A

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Target Background

Function
Unknown. Cellular response protein to viral infection.
Gene References into Functions
  1. GOLM1 acts as a critical oncogene by promoting prostate cancer cell proliferation, migration and invasion, and inhibiting apoptosis. GOLM1 plays oncogenic functions mainly through activating PI3K-AKT-mTOR signaling pathway. PMID: 29181846
  2. Results showed that GOLM1 was significantly upregulated in both LUAD and LUSC tissues compared to the normal controls. However, GOLM1 expression was higher in LUAD tissues than in LUSC tissues. PMID: 29843532
  3. GOLM1 was highly expressed in lung adenocarcinoma cells and associated with low survival ratio and higher grade malignancy; overexpression of GOLM1 promoted the cell proliferation. PMID: 29710483
  4. PDGFA/PDGFRalpha-regulated GOLM1 promotes glioma progression possibly through activation of a key signaling kinase, AKT PMID: 29282077
  5. GP73 is an effective and reliable serological marker for the diagnosis of advanced fibrosis and prediction of appearance of cirrhosis PMID: 29256057
  6. GOLPH2 promotes the progression of pancreatic ductal adenocarcinoma. PMID: 29344673
  7. serum GP73 is an accurate serum marker for significant fibrosis in chronic HBV infection, with higher accuracy than APRI and FIB-4. Serum GP73 is potentially a complementary tool for TE when evaluating the necessity of antiviral treatment, particularly in patients without definite antiviral indication. PMID: 29082644
  8. The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
  9. The mechanism of hepatocarcinogenesis and a promising blueprint for miR-493-5p-GP73 axis-oriented treatment of HCC. PMID: 28419971
  10. GP73 unglycosylation is associated with hepatocellular carcinoma cell motility and invasiveness. PMID: 26993603
  11. The GOLPH2 is a novel marker for non-small-cell lung cancer. PMID: 28880107
  12. Findings suggest that GP73 silencing through siRNA delivery may provide a low-toxicity therapy for the inhibition of tumor proliferation and metastasis. PMID: 26870893
  13. Both gain- and loss-of-function studies determine that GOLM1 acts as a key oncogene by promoting HCC growth and metastasis. PMID: 27569582
  14. A cytokine QTL at the NAA35-GOLM1 locus markedly modulated interleukin (IL)-6 production in response to multiple pathogens and was associated with susceptibility to candidemia. PMID: 27376574
  15. knock-down of GP73 down-regulates HCV infection and replication in Huh7-MAVSR cells and primary human hepatocytes PMID: 28394926
  16. Studies indicate that golgi membrane protein 1 (GOLM1) may promote HCC by regulation of epidermal growth factor receptor (EGFR) recycling, and suggest that therapeutic targeting of GOLM1 may be a potential strategy for combating hepatocellular carcinoma (HCC) metastasis. PMID: 27858335
  17. these studies revealed that GP73 promotes hepatitis C virus (HCV) secretion by directly mediating the interaction of ApoE with HCV replication complex through binding with HCV NS5A. PMID: 27697522
  18. Significantly higher serum levels of GP-73 and PIVKA-II were detected in the hepatocellular carcinoma patients than in controls. PMID: 28276727
  19. The critical role of GP73 in hepatocellular carcinoma invasion, epithelial-mesenchymal transition and metastasis PMID: 28075476
  20. Results show that overexpression of GP73 in hepatocellular carcinoma cell (HCC) line retrieved the expression of epithelial-mesenchymal transition (EMT) markers, and promoted cell motility and invasion. High expression of GP73 was also found in HCC tissues with metastasis providing evidence for an important role of GP73 in HCC metastasis. PMID: 26820712
  21. Serum GP73 had limited diagnostic value for HBV-related liver cancer. The combined use of serum GP73 and AFP levels improved the diagnostic efficacy. PMID: 26617863
  22. GP73 enhances MMP-13 expression through cAMP responsive element binding protein (CREB)-mediated transcription activation. Levels of GP73 and MMP-13 are increased and positively correlated in human HCC tissues. Augmented MMP-13 potentiates HCC cell metastasis. PMID: 26378022
  23. the specific antibody could block the binding of A10-2 to GP73, and the specific binding of A10-2 to GP73 was also supported by the observation that several tumor cell lines exhibited variable expression level of GP73. PMID: 26583119
  24. Transcatheter arterial chemoembolization significantly increased GP73 expression in patients with hepatocellular carcinoma. PMID: 26256086
  25. GP73 and AFP-L3 are superior biomarkers to aid the diagnosis of small PHC with negative AFP. The combined detection of biomarkers improved the diagnostic accuracy. PMID: 26406957
  26. The results of this meta-analysis showed that serum GP73 + AFP exhibited significantly higher diagnostic accuracy for HCC than did serum GP73 or AFP alone. PMID: 26441340
  27. Sublingual vein parameters and AFP, AFP-L3, and GP73 serum gene expression facilitate early diagnosis of patients with hepatocellular carcinoma. PMID: 26125916
  28. GOLPH2 may be a useful tissue biomarker for oesophageal disease. PMID: 26461057
  29. a GP73 assay is not suitable for discriminating between primary malignant and benign tumors of the liver. PMID: 25033446
  30. Increased expression of GP73 promotes proliferation and migration of hepatocellular carcinoma cell lines and growth of xenograft tumors in mice. PMID: 25980751
  31. Results show that GOLPH2 is upregulated in Gastric cancer and correlated with shorter overall survival suggesting that GLPH2 is associated with the development and progression of the disease. PMID: 25119897
  32. serum GP73 was found to be correlated with liver pathological grading and staging in patients with CHB, and may be an effective indicator for the evaluation of disease progression. PMID: 25524053
  33. GP73 may play an important role in the inhibitory regulation of autophagy. PMID: 25527157
  34. GOLM1 was directly regulated by miR-27b in prostate cancer cells PMID: 25115396
  35. The variation trend of gp73 in chronic liver disease may indicate that monitoring of serum gp73 is helpful to diagnose cirrhosis in population with chronic HBV infection. PMID: 25168922
  36. GP73 might play an important role in proliferation and apoptosis in hepatocellular carcinoma cells. PMID: 25170213
  37. Studied the serum levels of GP73 in patients with HBV-ACLF. Found concentrations of GP73 in patients with HBV-ACLF (285.3 +/- 128.5 ng/mL) were markedly higher than HCC patients (159.1 +/- 105.8 ng/mL),CHB patients (64.65 +/- 44.99 ng/mL),and healthy controls. PMID: 24560809
  38. The expression levels of GOLPH2 and IL-12A were negatively correlated. PMID: 24289573
  39. The results demonstrate the critical function of GP73 in HCV secretion and provide new insights into the therapeutic design of antiviral strategies. PMID: 24608522
  40. Data demonstrate that chimera GOLM1-MAK10 encodes a secreted fusion protein. Mechanistic studies reveal that GOLM1-MAK10 is likely derived from transcription read-through/splicing rather than being generated from a fusion gene. PMID: 24243830
  41. Overexpression of GOLM1 is associated with hepatocellular carcinoma. PMID: 23838921
  42. Loss of the tumor-suppressive miR-143/145 cluster enhanced cancer cell migration and invasion in PCa through directly regulating GOLM1. PMID: 24284362
  43. GP73 is involved in the regulation of epithelial-mesenchymal transformation and invasiveness of hepatocellular carcinoma. PMID: 24313979
  44. The usefulness of serum Transforming growth factor-beta1 (TGF-beta1), Glypican-3 (GPC3), and Golgi protein-73 (GP73) mRNAs as early biomarkers in HCC Egyptian patients, was investigated. PMID: 24186850
  45. GP73 is a potential marker for evaluating AIDS progression and antiretroviral therapy efficacy. PMID: 24068434
  46. Serum GP73 has a relatively high diagnostic accuracy in primary hepatic carcinoma with better sensitivity and high-specificity than alpha-fetoprotein.[review] PMID: 24779292
  47. Serum GP73 were increased in patients with fatty liver disease. PMID: 24579464
  48. GP73 is down-regulated in gastric cancer and associated with tumor differentiation. PMID: 23742050
  49. GP73 may be a marker for diagnosing significant fibrosis in patients with chronic HBV infections, and may be a new contributor to fibrogensis. PMID: 23418424
  50. GP73 content of liver cancer patients was significantly higher than the chronic hepatitis, cirrhosis patients and controls. PMID: 23627036

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Subcellular Location
Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein. Note=Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
Protein Families
GOLM1/CASC4 family
Tissue Specificity
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in n
Database Links

HGNC: 15451

OMIM: 606804

KEGG: hsa:51280

STRING: 9606.ENSP00000373363

UniGene: Hs.494337

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