GSTO1 Antibody

Datasheet
Code CSB-RA009986A0HU
Size US$350
Uniprot No. P78417
Image
  • Western Blot
    Positive WB detected in: Jurkat whole cell lysate, PC3 whole cell lysate, Raji whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
    All lanes: GSTO1 antibody at 1.25μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28, 24, 25 KDa
    Observed band size: 28 KDa

  • IHC image of CSB-RA009986A0HU diluted at 1:125 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA009986A0HU diluted at 1:125 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-RA009986A0HU at 1:41, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from human GSTO1
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Relevance Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroascorbate reductase, GSTO1, GSTTLP28
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Tags & Cell Markers
Gene Names GSTO1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.
Subcellular Location Cytoplasm, cytosol
Protein Families GST superfamily, Omega family
Tissue Specificity Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.
Database Links

HGNC: 13312

OMIM: 605482

KEGG: hsa:9446

STRING: 9606.ENSP00000358727

UniGene: Hs.190028

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