Product Details

Uniprot No.

P01903

Target Names

HLA-DRA

Alternative Names

HLA class II histocompatibility antigen, DR alpha chain (MHC class II antigen DRA), HLA-DRA, HLA-DRA1

Species Reactivity

Human

Immunogen

A synthesized peptide from human HLA-DRA protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

HLA-DRA encodes the alpha chain of the MHC class II DR heterodimer, a critical component of the adaptive immune system responsible for presenting processed antigens to CD4+ T helper cells. This molecule plays a central role in initiating and regulating immune responses, making it an essential target for researchers investigating antigen presentation, autoimmune disorders, transplant rejection, and tumor immunology. Unlike the highly polymorphic beta chain, the alpha chain is relatively invariant, providing a stable marker for studying MHC class II-expressing cells including dendritic cells, macrophages, and B lymphocytes.

This recombinant monoclonal antibody, clone 6C11, offers the consistency and reproducibility that demanding immunological research requires. Because the antibody sequence is defined and produced recombinantly in rabbit host cells, you can expect identical performance across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The affinity-purified rabbit IgG format ensures high specificity for your human HLA-DRA detection needs.

Validation in western blot applications demonstrates reliable detection in Raji whole cell lysate, a well-characterized B lymphoblastoid cell line with robust MHC class II expression. The observed band at approximately 35 kDa, compared to the predicted 29 kDa molecular weight, reflects the glycosylated state of the mature protein as it exists in cellular contexts. Recommended working dilutions of 1:500 to 1:2000 for western blotting provide flexibility to optimize signal-to-background ratios for your specific experimental conditions, with additional utility confirmed for ELISA applications.

This antibody serves researchers exploring immune cell biology, cancer immunotherapy mechanisms, and inflammatory disease pathways where MHC class II expression patterns inform both basic understanding and therapeutic development.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.

Gene References into Functions

This study demonstrated that only the non-suicidal depressed subgroup revealed significantly lower microglial reaction, i.e., a decreased density of HLA-DR positive microglia versus both depressed suicide victims and controls. PMID: 28229240
Low HLA-DRA expression is associated with sepsis. PMID: 28771573
In this prospective study we evaluated dynamic changes in monocyte HLA-DR expression during sepsis in relation to changes in HLA-DRA gene expression and Class II transactivator (CIITA), measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). PMID: 27144640
Case-control study in Han Chinese from the southwest of the country suggests that risk of ALS is increased by the AA genotype at rs9268856 in the HLA-DRA/HLA-DRB5 gene, but not by the SNPs rs9268877 in HLA-DRA/HLA-DRB5, rs1980493 in BTNL2 or rs302668 in RAB38/CTSC PMID: 28131168
Kaposi's sarcoma-associated herpesvirus RTA not only downregulated HLA-DRalpha at the protein level through direct binding and degradation through the proteasome pathway but also indirectly downregulated the protein level of HLA-DRalpha by enhancing the expression of MARCH8, a member of the membrane-associated RING-CH (MARCH) proteins. PMID: 27356905
The frequency of CD14(+)HLA-DR-/low MDSCs could be considered as a poor prognostic predictor in small-cell lung cancer (SCLC) and the elimination of MDSCs during medical interventions may improve the prognosis of SCLC patients. PMID: 25792471
This meta-analysis showed that the HLA-DRA rs3129882 A/G polymorphism was not a risk factor for Parkinson's disease in Chinese. PMID: 24849299
CD14+HLA-DR(-/low) cells were significantly increased in gastric cancer tissue and were shown to have a critical role in CD4+T-cell immunosuppression. PMID: 25695487
No significant association was found for HLA-DRA/PARK18 rs3129882 polymorphism and Parkinson's disease risk (meta-analysis). PMID: 25720714
The results suggest that rs3129882 polymorphism in HLA-DR may be a risk factor for Parkinson's disease in Iranian. PMID: 25319953
Increased CD14+ monocytes with loss of HLA-DR expression were seen in patients with higher stage disease, more aggressive pathology, and in relapse or refractoriness to treatment. PMID: 24636145
HLA-DRA variants predict penicillin allergy in genome-wide fine-mapping genotyping PMID: 25224099
The effects of rs11248051 and rs1564282 variants of GAK, and the rs3129882 variant of HLA-DRA, were investigated in Parkinson's disease patients. PMID: 24039160
The SNP rs3129882 in intron 1 of HLA-DRA has been shown to be most robustly associated with the Parkinson's disease. PMID: 23083294
suggest that binding of MAM to HLA-DR leads to a conformational change in MAM structure allowing its interaction with TLR2 and TLR4 and a better recognition by T cells. PMID: 24493819
The HLA-DRA variation rs3129882 is not associated with Parkinson's disease in Sweden.( PMID: 23579001
The expression of human leucocyte antigen (HLA)-DR in epithelial cells and cluster of differentiation (CD8)-positive lymphocytes as possible markers of chronic ocular graft versus host disease, was investigated. PMID: 23878500
HLA-DR4, PAD4 and STAT4 are overexpressed in rheumatoid arthritis and may be involved in the pathogenesis of RA. PMID: 20584675
2 SNPs in the HLA-DRA gene were studied in Caucasian patients from 2 large studies of alcohol dependence. rs2239803 & rs4935356 were associated with AD in both cohorts. PMID: 22890421
Data indicate that 25 +/- 1.3% of CD74 and 17 +/- 0.3% of HLA-DR are colocalized. PMID: 22889831
In this study observe no association between this single nucleotide polymorphism of HLA-DRA and Parkinson's disease in a Taiwanese population. PMID: 22243834
genetic polymorphism is associated with the presence of nasal polyposis in asthmatic patients PMID: 22391069
The rs3129882 noncoding variant in intron 1 of HLA-DRA is associated with late-onset sporadic Parkinson disease in a Chinese Han population. PMID: 21791235
small interfering RNA-mediated knock-down of AIM2 resulted in reduced expression of HLA-DRA, HLA-DRB and CIITA in IFN-gamma-treated cells PMID: 21804607
SNP1 is a Parkinson's disease-associated variant in HLA-DRA and is associated with HLA-DRA, DRB5 and DQA2 gene expression. PMID: 22096524
Molecular Dynamics simulations of the MuBetaRho 83-99 (Phe91) and MuBetaRho 83-99 (Tyr91) peptide analogues in complex with HLA-DR2 (DRA, DRB1*1501) and T-cell receptors were performed. PMID: 21898163
This study indicated that the association between rs3129882 and parkinson disease remains unclear and requires further study. PMID: 21482477
HLA-DR(+) endothelial cells regulate the local inflammatory allogeneic response, promoting either an IL-6/STAT-3-dependent Th17 response or a contact-CD54-dependent regulatory response according to the cytokine environment. PMID: 21282653
Upregulation of HLA-DRA is associated with acute pancreas allograft rejection. PMID: 20497195
The skeletal muscles of myasthenia gravis up regulation of MHC class @@. PMID: 20546939
predictive value of expression in diagnosis of acute promyelocytic leukemia PMID: 20232575
Continuous veno-venous hemofiltration is effective in removal of many plasma cytokines and in improvement of monocyte HLA-DR expression in septic patients. PMID: 20202859
DNMT1 and HLA-DRalpha are prognostic factors for hepatocelluar carcinoma. PMID: 18931722
Data show that pollen grains triggered the production of IL-8, TNF-alpha, IL-6 and strongly upregulated the membrane expression of CD80, CD86, CD83, HLA-DR and caused only a slight increase in the expression of CD40. PMID: 20118277
Findings show that Sug1 is crucial for regulating histone H3K4me3 and H3R17me2 at the cytokine inducible MHC-II and CIITA promoters. PMID: 19660582
Studies indicate that five SNPs showed genome-wide significant association with MS: HLA-DRA, IL7R, IL2RA, CD58 and CLEC16A. PMID: 19834503
P. gingivalis membrane vesicles apparently inhibited IFN-gamma-induced MHC class II (HLA-DRalpha) by disrupting the IFN-gamma signaling transduction pathway. PMID: 11854199
IFN-gamma-induced HLA-DRA expression was inhibited by nitric oxide (NO) and antioxidants such as superoxide dismutase, catalase, pyrrolidine dithiocarbamate, and N-acetylcysteine PMID: 12006557
The donor TNFRSF6 polymorphism dierctly or indirectly influences acute kidney rejection episodes. PMID: 14736971
different regions of the cytoplasmic tails are involved in the association and the recruitment of HLA-DR(A & B1) into different compartments. HLA-DP and -DR interact differently with the cytoskeleton. PMID: 14976194
Data show that Oct-1 occupies the endogenous HLA-DRA promoter when the HLA-DRA promoter is inactive in retinoblastoma protein-defective cells. PMID: 15105429
Overexpression of HLA-DRA is associated with ovarian and other cancers PMID: 15467430
Downregulation of HLA-DRA is associated with early intrahepatic recurrence of hepatocellular carcinoma PMID: 15688398
Alleles of IL2RA and IL7RA and those in the HLA locus are identified as heritable risk factors for multiple sclerosis. PMID: 17660530
HLA-DR alpha 2 domain (sHLA-DRalpha2) induces negative signals by engaging TIRC7 on lymphocytes, inhibiting proliferation and inducing apoptosis in CD4+ and CD8+ T-cells via activation of the intrinsic pathway PMID: 18270567
although lysine 219 is absolutely required for modification of DRalpha, other features of the DRalpha tail act to limit the extent of ubiquitination. PMID: 19117940
T cell receptor view of HLA-DRA in pocket 9 shows that the arrangement of residues in this pocket promotes anchoring of either large aliphatic or aromatic amino acids at this position. PMID: 19648278

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Early endosome membrane; Single-pass type I membrane protein. Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Autolysosome membrane; Single-pass type I membrane protein.

Protein Families

MHC class II family

Tissue Specificity

Expressed in professional APCs: macrophages, dendritic cells and B cells (at protein level). Expressed in thymic epithelial cells (at protein level).

Database Links

HGNC: 4947

OMIM: 142860

KEGG: hsa:3122

STRING: 9606.ENSP00000378786

UniGene: Hs.520048

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Code	CSB-RA188867A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Raji whole cell lysate(30µg) All lanes: HLA DRA antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 29 kDa Observed band size: 35 kDa Exposure time：2min40s
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HLA-DRA Recombinant Monoclonal Antibody

Product Details

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Target Background