Code | CSB-RA010429A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:500 |
The production of the histone H4 recombinant monoclonal antibody starts with the isolation of genes responsible for encoding the HIST1H4A antibody from rabbits previously immunized with a synthesized peptide derived from the human HIST1H4A protein. These HIST1H4A antibody genes are then meticulously cloned into specialized expression vectors. Following this genetic modification, the modified vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. After this cultivation phase, the histone H4 recombinant monoclonal antibody is subjected to a rigorous purification process utilizing affinity chromatography techniques, effectively separating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a battery of tests, including ELISA, IHC, and FC tests, conclusively confirming its ability to interact effectively with the human histone H4.
Histone H4, as a crucial component of chromatin, participates in various cellular processes that govern gene expression, DNA integrity, and cell division. Its dynamic interactions with DNA and various post-translational modifications make it a key player in epigenetic regulation.
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