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IL34 Recombinant Monoclonal Antibody

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Datasheet
Code CSB-RA751146MA1HU
Size $49.9
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Image
  • Overlay Peak curve showing MCF-7 cells stained with CSB-RA751146MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Q6ZMJ4
Target Names
IL34
Alternative Names
C16orf77 antibody; Chromosome 16 open reading frame 77 antibody; FPT025 antibody; IL 34 antibody; IL-34 antibody; Il34 antibody; IL34_HUMAN antibody; Interleukin 34 antibody; Interleukin-34 antibody; Interleukin34 antibody; MGC34647 antibody
Species Reactivity
Human
Immunogen
Recombinant Human IL34 protein
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
hIgG1
Clone No.
17G7
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, FC
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Protocols
ELISA Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs
Antibody FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Interleukin-34 represents a fascinating addition to the cytokine landscape, functioning as an alternative ligand for the CSF-1 receptor alongside macrophage colony-stimulating factor. This dual-ligand system provides researchers with unique opportunities to investigate tissue-specific macrophage development, microglial homeostasis in the central nervous system, and inflammatory responses across diverse biological contexts. IL-34's distinct expression patterns and signaling kinetics compared to CSF-1 make it a compelling target for studies exploring immune cell differentiation, neuroinflammation, and tumor microenvironment dynamics.

This recombinant monoclonal antibody, clone 17G7, offers the reproducibility and consistency that demanding experimental workflows require. Produced using recombinant technology with a defined human IgG1 framework, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The sequence-defined nature of this antibody ensures that your results today will be reproducible months or years from now, providing a stable foundation for building upon your findings.

Validated for ELISA and flow cytometry applications, this antibody demonstrates reliable performance for detecting human IL-34. Flow cytometry validation using MCF-7 cells confirms specific intracellular staining, with clear signal separation from isotype control when cells are fixed and permeabilized. The recommended dilution range of 1:50 to 1:200 for flow cytometry provides flexibility to optimize signal-to-noise ratios across different experimental conditions and cell types.

Whether investigating macrophage biology, exploring CSF-1R signaling pathways, or characterizing IL-34 expression in disease models, this antibody delivers the specificity and batch consistency essential for generating publication-quality data in immunology and cancer research applications.

Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
Gene References into Functions
  1. Study found that high IL-34 levels in synovial fluid were significantly associated with the radiographic and symptomatic severity of knee osteoarthritis. PMID: 30159102
  2. Data showed that single expression of M-CSF or IL-34 can be observed in lung cancer tissues and correlated with poor survival. Additionally, their high co-expression correlates with disease stages and poor survival. Thus, evaluating the expression of both M-CSF and IL-34 may help to estimate disease progression and malignant degree in lung cancer patients. PMID: 29323162
  3. this study shows that IL-34 regulates IL-6 and IL-8 production in human lung fibroblasts via MAPK, PI3K-Akt, JAK and NF-kappaB signaling pathways PMID: 29857241
  4. Study indicates that IL-34 can be an indicator of liver inflammation and fibrosis in patients with chronic hepatitis B virus infection. PMID: 29599606
  5. In conclusion, elevated serum IL-34 levels were demonstrated to be independently associated with renal insufficiency and coronary artery disease in patients with chronic heart failure, regardless of the systolic function. PMID: 27982136
  6. Data indicate that the interleukin 34 (IL-34) is a feasible diagnostic marker of liver fibrosis in non-alcoholic fatty liver disease (NAFLD) patients. PMID: 27363523
  7. Findings uncover a novel function for IKKbeta/mHTTx1 interactions in regulating IL-34 production, and implicate a role for IL-34 in non-cell-autonomous, microglial-dependent neurodegeneration in HD. PMID: 28973132
  8. The IL-34/STAT3/miR-21 pathway is crucial for the survival of synovial fibroblasts in rheumatoid arthritis PMID: 27084907
  9. Data show that both serum interleukin-34 (IL-34) and IL-34 mRNA in mononuclear leukocytes (PBMCs) in chronic hepatitis B virus (HBV) patients was significantly decreased compared to the healthy controls. PMID: 28614380
  10. pathogenic role for IL34 in mediating immunosuppression and chemoresistance and identify it as a tractable target for anticancer therapy. PMID: 27550451
  11. The current study aimed to assess the IL-34 expression in response to two members of the transforming growth factor (TGF)-beta family, TGF-beta1 and bone morphogenetic protein (BMP)-2, in synovial fibroblasts from rheumatoid arthritis patients. PMID: 27865758
  12. The receiver operating characteristic (ROC) curve analysis has shown that IL-34 has more discriminatory power than C-reactive protein (CRP) for the risk of diabetic complications. The cut-off value for IL-34 was established as 91.2 pg/mL. The gist of our research was identification of IL-34 as an additional potential inflammatory biomarker for the prediction of the risk of vascular diabetic complications. PMID: 26849008
  13. miR-28-5p-IL-34-macrophage feedback loop modulates hepatocellular carcinoma metastasis PMID: 26754294
  14. findings demonstrated that M-CSF binds to IL-34; molecular docking studies predicted the formation of a heteromeric M-CSF/IL-34 cytokine PMID: 26095744
  15. These results suggest that IL-34, a novel osteoclastogenic cytokine, plays a role in rheumatoid arthritis-associated joint damage and is a potential biomarker for predicting subsequent radiographic progression in patients with RA. PMID: 25027626
  16. IL-34 is expressed by human FOXP3+CD45RCloCD8+ and CD4+ Tregs and markedly inhibited alloreactive immune responses. PMID: 26389674
  17. This paper provides evidence of alternative binding of IL-34 to chondroitin sulphates and syndecan-1 at the cell surface that modulates M-CSFR activation. PMID: 25662098
  18. In vitro and in vivo experiments indicate that IL-34 expression is regulated by TNF-a and IL-1b and that its overexpression is associated with an increase in osteosarcoma growth and metastasis. PMID: 25471534
  19. the expression pattern of IL-34 in ileum and colon and suggest IL-34 as a new modulator of inflammation in inflammatory bowel disease PMID: 25896238
  20. IL-34 is up-regulated in inflammatory bowel disease and suggest a role for this cytokine in sustaining the inflammatory responses in this disease PMID: 25800277
  21. IL-34 is induced by IL-22 in the inflammatory cascade in response to IAV infection. PMID: 25415279
  22. IL-34 expression in human gingival fibroblasts, stimulated by TNF-alpha and IL-1beta PMID: 24339952
  23. potent profibrotic factor in hepatitis C virus liver fibrosis PMID: 25066464
  24. IL-34 is associated with insulin resistance. PMID: 24712570
  25. IL-34 promotes the development, survival, and function of microglia and Langerhans cells; therefore, this cytokine may predominately function in brain and skin biology.[review] PMID: 24737461
  26. Circulating IL-34 levels in rheumatoid arthritis correlated with autoantibody production. PMID: 23996288
  27. This study is the first to illustrate downstream transcriptional profiles and pathways of IL-34 in comparison with CSF-1 and identify notable differences in CCR2 expression. PMID: 23684409
  28. These findings suggest that IL-34 may play a role in the pathogenesis of rheumatoid arthritis. PMID: 23421370
  29. Data suggest that CSF-1R-independent actions of IL-34 via receptor-type protein-tyrosine phosphatase zeta (PTP-zeta) might be considered in evaluating IL-34 roles in development and disease. PMID: 23744080
  30. the feline CSF-1R was cloned and the responsiveness to CSF-1 and IL-34 from a range of species, was examined. PMID: 23260168
  31. IL-34 levels were significantly increased in patients with coronary artery disease (CAD), and positively correlated with hs-CRP levels, suggesting that IL-34 may be an independent predictor of CAD. PMID: 23206468
  32. IL-34 as a nonredundant cytokine for the development of Langerhans cells during embryogenesis as well as for their homeostasis in the adult skin. PMID: 23177320
  33. Data suggest a discrete role of IL-34 in inflammatory rheumatoid arthritis (RA) diseases. PMID: 22264405
  34. study identifies IL-34 expression in the synovial tissue of patients with arthritis; this cytokine, as a downstream effector of TNFalpha and IL-1beta, may contribute to inflammation and bone erosions in rheumatoid arthritis PMID: 22039170
  35. The different spatiotemporal expression of IL-34 and CSF-1 allows for complementary activation of the CSF-1R in developing and adult tissues. PMID: 20504948
  36. Discovered IL-34 and its receptor CSF1 by functional screening of the extracellular proteome. PMID: 18467591

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Subcellular Location
Secreted.
Protein Families
IL-34 family
Tissue Specificity
Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate
Database Links

HGNC: 28529

OMIM: 612081

KEGG: hsa:146433

STRING: 9606.ENSP00000288098

UniGene: Hs.461214

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