Code | CSB-RA157463A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
The INPP5D recombinant antibody is prepared using protein and DNA recombinant technologies. First, a synthesized peptide derived from human INPP5D is used to immunize mice. After a specific period, the spleen of mice is aseptically removed, and total RNA is extracted from the spleen cells. The extracted RNA is reverse transcribed to cDNA, which is then used as a template for PCR amplification of the INPP5D antibody gene. The INPP5D antibody gene is then cloned into a vector, which is transfected into host cells for culture. The INPP5D recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. This antibody undergoes rigorous verification and can be utilized in ELISA, WB, IHC, and IP experiments for the detection of human INPP5D protein.
INPP5D, also known as SHIP-1, is a phosphatase enzyme that plays a key role in regulating intracellular signaling pathways in various cells, including immune cells.
INPP5D dephosphorylates the PIP3 to generate PIP2, thereby inhibiting downstream signaling events mediated by the PI3K pathway. This negative regulation of PI3K signaling by INPP5D is important for the maintenance of immune cell homeostasis and the prevention of autoimmunity.
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