Product Details

Uniprot No.

Target Names

Alternative Names

KIR3DL2 antibody; CD158K antibody; NKAT4Killer cell immunoglobulin-like receptor 3DL2 antibody; CD158 antigen-like family member K antibody; MHC class I NK cell receptor antibody; Natural killer-associated transcript 4 antibody; NKAT-4 antibody; p70 natural killer cell receptor clone CL-5 antibody; p70 NK receptor CL-5 antibody; CD antigen CD158k antibody

Species Reactivity

Human

Immunogen

Recombinant Human KIR3DL2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

8D10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:20-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

KIR3DL2, also designated CD158k, belongs to the killer cell immunoglobulin-like receptor family and plays a critical role in regulating natural killer cell function through recognition of MHC class I molecules. This inhibitory receptor has garnered significant research interest in immunology, particularly in studies examining NK cell education, tumor immunosurveillance, and certain T-cell lymphomas where KIR3DL2 serves as both a diagnostic marker and therapeutic target.

This recombinant monoclonal antibody, clone 8D10, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures lot-to-lot reproducibility, allowing you to generate comparable data across extended studies without concerns about antibody drift or batch variation. The human IgG1 isotype format and affinity chromatography purification deliver a reagent ready for demanding experimental workflows.

Validation studies demonstrate robust performance in flow cytometry applications, where the antibody clearly distinguishes KIR3DL2-expressing cells from negative populations. Testing with HEK-293T cells stably transfected with human KIR3DL2 showed strong positive staining compared to untransfected controls, confirming specificity for the target. Functional ELISA data further supports binding activity, with EC50 values in the 14.18–23.93 ng/mL range against immobilized recombinant human KIR3DL2 protein, indicating high-affinity target recognition.

For flow cytometry experiments, dilutions ranging from 1:20 to 1:200 provide flexibility to optimize signal-to-noise ratios across different sample types and instrument configurations. The unconjugated format allows pairing with your preferred secondary detection system for multicolor panel design. This antibody supports investigations into NK cell biology, immune checkpoint mechanisms, and lymphoma research where KIR3DL2 expression patterns inform both basic science and translational applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Receptor on natural killer (NK) cells and T cells for MHC class I molecules. Upon binding of peptide-free HLA-F open conformer, negatively regulates NK and T cell effector functions. Acts as a receptor on astrocytes for HLA-F. Through interaction with HLA-F, may protect motor neurons from astrocyte-induced toxicity.

Gene References into Functions

We show that KIR3DL2 expression is the most sensitive diagnostic criterion of Sezary syndrome when compared with all other available biological criteria. KIR3DL2 therefore represents a valuable tool for routine use as a clinical parameter at diagnosis, for prognosis and during patient follow-up PMID: 28119365
results identified significant KIR3DL2 expression in all cutaneous T-cell lymphomas subtypes PMID: 29089310
Overexpression of a single MHCI molecule, HLA-F, protects human MNs from ALS astrocyte-mediated toxicity, whereas knockdown of its receptor, the killer cell immunoglobulin-like receptor KIR3DL2, on human astrocytes results in enhanced motor neurons death PMID: 26928464
KIR-3DL2 binding to HLA-B27 licenses Th17 cell differentiation in spondyloarthritis. PMID: 26841353
In early axial spondyloarthritis and ankylosing spondylitis Dutch patients, no copy number changes were found for KIR3DL2. PMID: 25940819
we show that the allele KIR3DL2*001 and the single nucleotide polymorphism 1190T (rs3745902) are associated with differential susceptibility to pemphigus PMID: 25867094
CD158k expression was identified on cutaneous CD4+ T cells in healthy individuals and also mycosis fungoides patients. PMID: 25044837
These findings provide novel insights about the molecular basis of KIR3DL2 binding to B27 PMID: 25582852
these data provide evidence for a possible role of KIR3DL2 in the maintenance of a high circulating malignant-cell burden by preventing activation-induced cell death. PMID: 25414436
Our results indicate that KIR3DL2 can directly promote Sezary syndrome malignant cell death through the use of CpG ODN. PMID: 25007046
our results show that a multistep gating of CD158k+ cells is reliable to assess tumor burden in case of Sezary syndrome. PMID: 25158034
our results offer preclinical proof of concept for the clinical development of IPH4102, a humanized monoclonal antibody that targets the immune receptor KIR3DL2,to treat patients with nced cutaneous T-cell lymphoma PMID: 25361998
KIR3DL2 binds to HLA-B27 dimers and free H chains more strongly than other HLA class I and promotes the expansion of T cells in ankylosing spondylitis. PMID: 23440420
PLS3, Twist, KIR3DL2 and NKp46 gene expression can model efficient molecular Sezary syndrome diagnosis. PMID: 23429988
most frequently expressed KIR receptor in transformed mycosis fungoides PMID: 22621189
results suggest that carriers of A allele in exon 3 of killer cell immunoglobulin-like receptor 3DL2(KIR3DL2) have a decreased susceptibility to preeclampsia PMID: 21406041
Enhanced proliferation, survival, and interleukin (IL)-17 production of KIR3DL2+ CD4 T cells stimulated with antigen-presenting cells expressing HLA-B27 homodimers suggest new therapeutic strategies in ankylosing spondylitis. PMID: 21248258
Data show direct binding of KIR3DL2 to ODNs and we show that the D0 domain is involved primarily in this interaction. PMID: 20147700
The A52G polymorphism in exon 3 and the frequencies of C32T in exon 9 of KIR3DL2 gene polymorphism are associated with the pathogenesis of pre-eclampsia. PMID: 19953898
All genotypes seen included genes for KIR3DL2. PMID: 12559621
IR3DL2/p140 engagement does not result into inhibitory (nor activatory) effects on tumor-specific cytotoxic T lymphocytes. PMID: 14562047
A high level of KIR3DL2 allelic polymorphism has been identified. PMID: 15304002
Receptor is a marker for the diagnosis of Sezary syndrome. PMID: 16962036
Five new alleles are reported, and four previously known ones are confirmed. PMID: 17661911
observation that CD4 positive, CD7 negative T-cells are mostly CD158k negative suggests that CD158k may be able to help identify and enumerate neoplastic T-cells in Sezary syndrome PMID: 18061949
Functional analysis of KIR3DL2-single positive natural killer (NK) cells reveals a subset that is hyporesponsive in individuals harboring cognate ligands HLA-A3/A11. PMID: 18941190
Anti-p140 autoantibodies represent a major autoantibody subset in juvenile dermatomyositis. PMID: 19479859
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Immunoglobulin superfamily

Tissue Specificity

Expressed in astrocytes.

Database Links

HGNC: 6339

OMIM: 604947

KEGG: hsa:3812

STRING: 9606.ENSP00000325525

UniGene: Hs.645228

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Code	CSB-RA012365MA1HU
Size	US$210
	Order now
Image	The Binding Activity of Human KIR3DL2 with Anti-KIR3DL2 Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KIR3DL2(CSB-MP012365HU1) at 2 μg/mL can bind Anti-KIR3DL2 recombinant antibody , the EC₅₀ is 14.18-23.93 ng/mL. Untransfected HEK-293T cells (green line) and transfected Human KIR3DL2 HEK-293T stable cells (red line) were stained with anti-KIR3DL2 recombinant antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
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KIR3DL2 Recombinant Monoclonal Antibody

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Target Background