Product Details

Uniprot No.

Q9UJU2

Target Names

LEF1

Alternative Names

Lymphoid enhancer-binding factor 1 (LEF-1) (T cell-specific transcription factor 1-alpha) (TCF1-alpha), LEF1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human LEF1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

LEF1 (Lymphoid enhancer-binding factor 1) serves as a critical transcription factor within the Wnt signaling pathway, where it partners with β-catenin to regulate genes essential for cell proliferation, differentiation, and survival. Its dysregulation has been implicated in various malignancies, making LEF1 a compelling target for researchers investigating cancer biology, stem cell maintenance, and developmental processes. The protein's role in T cell development and its expression patterns in lymphoid tissues further extend its relevance to immunological research.

This recombinant monoclonal antibody, clone 13B7, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma-derived sources, researchers benefit from lot-to-lot uniformity that supports longitudinal studies and ensures comparable results across experiments. The rabbit IgG format, purified by affinity chromatography, delivers the specificity needed for reliable target detection in human samples.

Validation through immunohistochemistry demonstrates this antibody's utility in formalin-fixed, paraffin-embedded tissue analysis. Testing in human tonsil tissue confirms detection in a lymphoid context where LEF1 naturally functions, while staining in human rectal cancer tissue showcases its applicability to oncology research where aberrant Wnt pathway activation is frequently observed. Working dilutions of 1:50 to 1:200 provide flexibility for optimization across different tissue types and detection systems.

Whether investigating Wnt pathway dynamics in developmental models, characterizing LEF1 expression in tumor specimens, or exploring its role in stem cell biology, this antibody provides a dependable tool for nuclear transcription factor detection in clinically and biologically relevant human tissues.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.; Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.; Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.; Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.

Gene References into Functions

Experimental analysis showed the importance of LEF1, ETV4 and FABP6 as three co-regulated prognostic markers in patients with colorectal cancer metastasis. PMID: 30193961
LEF-1 and CDX2 performed at least as well as beta-catenin, if not better, as a diagnostic marker for pilomatrical carcinomas PMID: 29369390
study identifies a novel mechanism of hepatocellular carcinoma inhibition through beta-catenin-independent Wnt signalling, which is regulated by WT1-associated LEF1 repression. The study also highlights mangiferin as a promising Wnt inhibitor for HCC treatment. PMID: 29953980
High LEF1 expression is associated with papillary thyroid carcinoma. PMID: 28677753
LEF1 was consistently expressed in the tubal-peritoneal junctions and all tubal intraepithelial lesions, independent of p53 status. PMID: 28664938
these data suggest that LEF1 rather has tumor suppressive functions and attenuates aggressiveness in a subset of RMS. PMID: 27965462
Positive LEF-1 favors a benign neoplasm. PMID: 28972308
Results show that high enhancer-binding factor-1 (LEF1) expression is associated with poor survival for chronic lymphocytic leukemia (CLL) patients. PMID: 26950276
Knockdown of GATA6 completely eliminated the effect of TCF1, while forced expression of GATA6 induced hESC differentiation PMID: 28943339
Lef1 and Tcf1 showed oncogenic effect in colonic carcinogenesis. Cellular context of miRNAs might play important roles in carcinogenesis by altering the expression pattern of Lef/Tcfs members. PMID: 27433921
Data indicate that microRNA miR-27a directly targets GSK-3beta and increases expression of beta-catenin and LEF1 in all-trans-retinoic acid (ATRA)-induced Hep2 cells. PMID: 28122350
and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
MicroRNA-557 might work as a tumor suppressor by negatively regulating the expression of lymphocyte enhancement factor 1 in lung cancer cells. PMID: 28639890
EVI1 transcription is directly regulated by LEF1/beta-catenin complex in myeloid blast crisis of chronic myeloid leukemia. Loss of p53 function as a key regulator for beta-catenin-EVI1 in myeloid blast crisis of chronic myeloid leukemia. PMID: 27908728
LEF1 is a sensitive and specific marker for chronic lymphocytic B-cell leukemia PMID: 28395058
a frequency of 4-9% expression of LEF1 bymantle cell lymphoma PMID: 28038713
The results suggest a role for beta-catenin/LEF1-mediated transcription in both malignant transformation and metastasis of prostate cancer PMID: 27067790
The results describe a novel mutation in LEF1 causing dysregulation of Wnt/B catenin signaling in eyelid sebaceous cancinoma PMID: 25639559
LEF-1 and MITF regulate tyrosinase gene transcription in vitro via binding to its promoter. PMID: 26580798
Methylprednisolone can suppress Wnt signaling pathway by down-regulating LEF-1 protein expression in chronic lymphocyte leukemia cells. PMID: 26339357
Overexpression of LEF1 is a favorable prognostic factor in childhood ALL. PMID: 25955539
Authors found that the N-terminus of delta-catenin bound to the middle region of LEF-1 unlike beta-catenin. Overexpressed delta-catenin entered the nucleus and inhibited LEF-1-mediated transcriptional activity in Bosc23 and DLD-1 cell lines. PMID: 25808920
high LEF1 expression and mutation are associated with high-risk leukemia and our results also revealed that LEF1 high expression and/or gain-of-function mutations are involved in leukemogenesis of ALL. PMID: 25942645
Study found a negative correlation between miR-34a and LEF1 expression in prostate cancer cell lines and tumors and that miR-34a regulated epithelial-mesenchymal transition through direct binding to LEF1 mRNA 3' UTR region and silencing its translation. PMID: 25587085
Our study suggests LEF1 expression in oral squamous cell carcinoma may play an important role in tumor progression and can be served as a predictor of poor prognosis for patients with oral squamous cell carcinoma. PMID: 24021930
Elevated TCF-1 and LEF-1 expression is characteristic of malignant gliomas PMID: 24858819
LEF-1 expression is associated with the presence of KRAS mutations and may have prognostic value as a trend of worse overall survival is seen in patients with LEF-1-positive colorectal carcinoma. PMID: 25394300
These results further implicate the CTNNB1/LEF1 transcriptional complex in the development of solid-pseudopapillary neoplasms of the pancreas PMID: 24658583
study identified TCF1 and LEF1 as Tax antagonistic factors in vivo, a fact which may critically influence the peripheral T-cell tropism of this virus PMID: 25646419
miR26b may act as a potential therapeutic agent in reducing cancer cell proliferation through repressing LEF1 activation of c-Myc and cyclin D1 expression PMID: 24785257
Tcf1 and Lef1 cooperate with Runx factors to achieve stable silencing of the Cd4 gene in CD8(+) T cells. PMID: 24847765
LEF-1 expression in both basal cell adenomas and basal cell adenocarcinomas preferentially over other salivary gland tumors suggesting some utility as a diagnostic marker PMID: 25497834
Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells PMID: 25596247
LEF1 expression is an independent prognostic factor in APL, and could be used in patients risk stratification. PMID: 24378360
Selenite caused CYLD upregulation via LEF1 and cIAP downregulation, both of which contribute to the degradation of ubiquitin chains on RIP1 and subsequent caspase-8 activation and colorectal tumor cell apoptosis. PMID: 24577083
Results indicate that lymphoid enhancer-binding factor 1 (LEF1) contributes to the pathophysiology of acute myeloid leukemia (AML) and could be a predictor of better treatment response. PMID: 23713453
the expression of LEF1 is associated with the progression of human renal cell carcinoma and LEF1 maybe involved in the development of RCC PMID: 24897388
By beta-catenin's association with LEF1 and BCL9-2/B9L. PMID: 24419084
LEF1 protein was overexpressed in colon cancer tissues and knockdown of LEF1 expression inhibited colon cancer growth in vitro and in vivo. PMID: 24098538
Studied the expression of TCF/LEF and SFRP family members (SFRP1 and SFRP3) to gain a better understanding of biological signaling pathways responsible for epidemiology and clinical parameters of clear cell RCC (cRCC). PMID: 23572277
High LEF1 expression is associated with prostate cancer. PMID: 23913826
DNA-dependent protein kinase catalytic subunit is a novel interaction partner of lymphocyte enhancer factor 1. PMID: 23325550
This study supports a functionally important role for LEF1 and its target genes in Burkitt's lymphoma. PMID: 23375451
this study demonstrated that miR-449a directly targets LEF-1, which in turn affects the expression of Sox 9, ultimately leading to the proper regulation of the differentiation and chondrogenesis PMID: 22769578
Nuclear LEF1 correlate with cerebral metastasis of lung adenocarcinomas. PMID: 23224985
LEF-1 might play an important role in colon carcinogenesis by acting as a regulator. PMID: 22639890
Lef1 is overexpressed in human endometrial tumors, consistent with it playing a role in gland proliferation. PMID: 22792274
An association between the maternal genotype and the occurrence of cleft lip or palate was observed at two polymorphic loci (rs10022956 and rs10025431), while a foetal-maternal effect modulating the risk of clefting was found at locus rs10025431. PMID: 21781440
n-Butyl benzyl phthalate promotes breast cancer progression by inducing expression of lymphoid enhancer factor 1 PMID: 22905168
interaction of HCLS1 with LEF-1 is essential for G-CSF-triggered myeloid differentiation PMID: 23001182

Hide All

Subcellular Location

Nucleus.

Protein Families

TCF/LEF family

Tissue Specificity

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several

Database Links

HGNC: 6551

OMIM: 153245

KEGG: hsa:51176

STRING: 9606.ENSP00000265165

UniGene: Hs.743478

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Code	CSB-RA561630A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB. IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
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LEF1 Recombinant Monoclonal Antibody

Product Details

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Target Background