Phospho-LEF1 (S42) Antibody

Code CSB-PA020264
Size US$100
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  • Western Blot analysis of 293 cells using Phospho-LEF-1 (S42) Polyclonal Antibody
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Product Details

Uniprot No.
Target Names
LEF1
Alternative Names
DKFZp586H0919 antibody; FLJ46390 antibody; LEF 1 antibody; LEF-1 antibody; Lef1 antibody; LEF1_HUMAN antibody; Lymphoid enhancer binding factor 1 antibody; Lymphoid enhancer-binding factor 1 antibody; T cell specific transcription factor 1 alpha antibody; T cell-specific transcription factor 1-alpha antibody; TCF 1 alpha antibody; TCF1 alpha antibody; TCF1-alpha antibody; TCF10 antibody; TCF1alpha antibody; TCF7L3 antibody; Transcription factor T cell specific 1 alpha antibody
Raised in
Rabbit
Species Reactivity
Human,Mouse,Rat
Immunogen
Synthesized peptide derived from Human LEF-1 around the phosphorylation site of S42.
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Isotype
IgG
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form
Liquid
Tested Applications
WB, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
ELISA 1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.; Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.; Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.; Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
Gene References into Functions
  1. Experimental analysis showed the importance of LEF1, ETV4 and FABP6 as three co-regulated prognostic markers in patients with colorectal cancer metastasis. PMID: 30193961
  2. LEF-1 and CDX2 performed at least as well as beta-catenin, if not better, as a diagnostic marker for pilomatrical carcinomas PMID: 29369390
  3. study identifies a novel mechanism of hepatocellular carcinoma inhibition through beta-catenin-independent Wnt signalling, which is regulated by WT1-associated LEF1 repression. The study also highlights mangiferin as a promising Wnt inhibitor for HCC treatment. PMID: 29953980
  4. High LEF1 expression is associated with papillary thyroid carcinoma. PMID: 28677753
  5. LEF1 was consistently expressed in the tubal-peritoneal junctions and all tubal intraepithelial lesions, independent of p53 status. PMID: 28664938
  6. these data suggest that LEF1 rather has tumor suppressive functions and attenuates aggressiveness in a subset of RMS. PMID: 27965462
  7. Positive LEF-1 favors a benign neoplasm. PMID: 28972308
  8. Results show that high enhancer-binding factor-1 (LEF1) expression is associated with poor survival for chronic lymphocytic leukemia (CLL) patients. PMID: 26950276
  9. Knockdown of GATA6 completely eliminated the effect of TCF1, while forced expression of GATA6 induced hESC differentiation PMID: 28943339
  10. Lef1 and Tcf1 showed oncogenic effect in colonic carcinogenesis. Cellular context of miRNAs might play important roles in carcinogenesis by altering the expression pattern of Lef/Tcfs members. PMID: 27433921
  11. Data indicate that microRNA miR-27a directly targets GSK-3beta and increases expression of beta-catenin and LEF1 in all-trans-retinoic acid (ATRA)-induced Hep2 cells. PMID: 28122350
  12. and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
  13. MicroRNA-557 might work as a tumor suppressor by negatively regulating the expression of lymphocyte enhancement factor 1 in lung cancer cells. PMID: 28639890
  14. EVI1 transcription is directly regulated by LEF1/beta-catenin complex in myeloid blast crisis of chronic myeloid leukemia. Loss of p53 function as a key regulator for beta-catenin-EVI1 in myeloid blast crisis of chronic myeloid leukemia. PMID: 27908728
  15. LEF1 is a sensitive and specific marker for chronic lymphocytic B-cell leukemia PMID: 28395058
  16. a frequency of 4-9% expression of LEF1 bymantle cell lymphoma PMID: 28038713
  17. The results suggest a role for beta-catenin/LEF1-mediated transcription in both malignant transformation and metastasis of prostate cancer PMID: 27067790
  18. The results describe a novel mutation in LEF1 causing dysregulation of Wnt/B catenin signaling in eyelid sebaceous cancinoma PMID: 25639559
  19. LEF-1 and MITF regulate tyrosinase gene transcription in vitro via binding to its promoter. PMID: 26580798
  20. Methylprednisolone can suppress Wnt signaling pathway by down-regulating LEF-1 protein expression in chronic lymphocyte leukemia cells. PMID: 26339357
  21. Overexpression of LEF1 is a favorable prognostic factor in childhood ALL. PMID: 25955539
  22. Authors found that the N-terminus of delta-catenin bound to the middle region of LEF-1 unlike beta-catenin. Overexpressed delta-catenin entered the nucleus and inhibited LEF-1-mediated transcriptional activity in Bosc23 and DLD-1 cell lines. PMID: 25808920
  23. high LEF1 expression and mutation are associated with high-risk leukemia and our results also revealed that LEF1 high expression and/or gain-of-function mutations are involved in leukemogenesis of ALL. PMID: 25942645
  24. Study found a negative correlation between miR-34a and LEF1 expression in prostate cancer cell lines and tumors and that miR-34a regulated epithelial-mesenchymal transition through direct binding to LEF1 mRNA 3' UTR region and silencing its translation. PMID: 25587085
  25. Our study suggests LEF1 expression in oral squamous cell carcinoma may play an important role in tumor progression and can be served as a predictor of poor prognosis for patients with oral squamous cell carcinoma. PMID: 24021930
  26. Elevated TCF-1 and LEF-1 expression is characteristic of malignant gliomas PMID: 24858819
  27. LEF-1 expression is associated with the presence of KRAS mutations and may have prognostic value as a trend of worse overall survival is seen in patients with LEF-1-positive colorectal carcinoma. PMID: 25394300
  28. These results further implicate the CTNNB1/LEF1 transcriptional complex in the development of solid-pseudopapillary neoplasms of the pancreas PMID: 24658583
  29. study identified TCF1 and LEF1 as Tax antagonistic factors in vivo, a fact which may critically influence the peripheral T-cell tropism of this virus PMID: 25646419
  30. miR26b may act as a potential therapeutic agent in reducing cancer cell proliferation through repressing LEF1 activation of c-Myc and cyclin D1 expression PMID: 24785257
  31. Tcf1 and Lef1 cooperate with Runx factors to achieve stable silencing of the Cd4 gene in CD8(+) T cells. PMID: 24847765
  32. LEF-1 expression in both basal cell adenomas and basal cell adenocarcinomas preferentially over other salivary gland tumors suggesting some utility as a diagnostic marker PMID: 25497834
  33. Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells PMID: 25596247
  34. LEF1 expression is an independent prognostic factor in APL, and could be used in patients risk stratification. PMID: 24378360
  35. Selenite caused CYLD upregulation via LEF1 and cIAP downregulation, both of which contribute to the degradation of ubiquitin chains on RIP1 and subsequent caspase-8 activation and colorectal tumor cell apoptosis. PMID: 24577083
  36. Results indicate that lymphoid enhancer-binding factor 1 (LEF1) contributes to the pathophysiology of acute myeloid leukemia (AML) and could be a predictor of better treatment response. PMID: 23713453
  37. the expression of LEF1 is associated with the progression of human renal cell carcinoma and LEF1 maybe involved in the development of RCC PMID: 24897388
  38. By beta-catenin's association with LEF1 and BCL9-2/B9L. PMID: 24419084
  39. LEF1 protein was overexpressed in colon cancer tissues and knockdown of LEF1 expression inhibited colon cancer growth in vitro and in vivo. PMID: 24098538
  40. Studied the expression of TCF/LEF and SFRP family members (SFRP1 and SFRP3) to gain a better understanding of biological signaling pathways responsible for epidemiology and clinical parameters of clear cell RCC (cRCC). PMID: 23572277
  41. High LEF1 expression is associated with prostate cancer. PMID: 23913826
  42. DNA-dependent protein kinase catalytic subunit is a novel interaction partner of lymphocyte enhancer factor 1. PMID: 23325550
  43. This study supports a functionally important role for LEF1 and its target genes in Burkitt's lymphoma. PMID: 23375451
  44. this study demonstrated that miR-449a directly targets LEF-1, which in turn affects the expression of Sox 9, ultimately leading to the proper regulation of the differentiation and chondrogenesis PMID: 22769578
  45. Nuclear LEF1 correlate with cerebral metastasis of lung adenocarcinomas. PMID: 23224985
  46. LEF-1 might play an important role in colon carcinogenesis by acting as a regulator. PMID: 22639890
  47. Lef1 is overexpressed in human endometrial tumors, consistent with it playing a role in gland proliferation. PMID: 22792274
  48. An association between the maternal genotype and the occurrence of cleft lip or palate was observed at two polymorphic loci (rs10022956 and rs10025431), while a foetal-maternal effect modulating the risk of clefting was found at locus rs10025431. PMID: 21781440
  49. n-Butyl benzyl phthalate promotes breast cancer progression by inducing expression of lymphoid enhancer factor 1 PMID: 22905168
  50. interaction of HCLS1 with LEF-1 is essential for G-CSF-triggered myeloid differentiation PMID: 23001182

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Subcellular Location
Nucleus.
Protein Families
TCF/LEF family
Tissue Specificity
Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several
Database Links

HGNC: 6551

OMIM: 153245

KEGG: hsa:51176

STRING: 9606.ENSP00000265165

UniGene: Hs.743478

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