Phospho-LEF1 (S42) Antibody

Code CSB-PA020264
Size US$167
Uniprot No. Q9UJU2
  • Western Blot analysis of 293 cells using Phospho-LEF-1 (S42) Polyclonal Antibody
Protocols Western Blotting(WB) Protocol
ELISA Protocol
Immunogen Synthesized peptide derived from Human LEF-1 around the phosphorylation site of S42.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Tested Applications WB,ELISA;WB:1/500-1/2000.ELISA:1/5000
Form Liquid
Conjugate Non-conjugated
Storage Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Alias LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
Immunogen Species Homo sapiens (Human)
Target Names LEF1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Subcellular Location Nucleus
Protein Families TCF/LEF family
Tissue Specificity Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several
Database Links

HGNC: 6551

OMIM: 153245

KEGG: hsa:51176

STRING: 9606.ENSP00000265165

UniGene: Hs.743478

Pathway Hippo signaling pathway
Wnt signaling pathway
Adherens junction

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