Code | CSB-RA697089A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The creation of the LTF recombinant monoclonal antibody involves a precise and comprehensive process to ensure its exceptional quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, where the synthesized peptide derived from human Lactoferrin acts as the immunogen. The RNA is extracted from the B cells and converted into cDNA through reverse transcription. The LTF antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently introduced into host cells via transfection, enabling the production of the LTF recombinant monoclonal antibody. Following a period of cell culture, the antibody is harvested from the cell culture supernatant and purified meticulously using affinity chromatography, resulting in a highly purified form suitable for various applications. Rigorous characterization assays, including ELISA and IHC analysis, are conducted to validate the antibody's specificity and functionality in detecting human LTF protein. The meticulous production process guarantees the development of a reliable and effective LTF recombinant monoclonal antibody, playing a pivotal role in diverse LTF-related research.
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