Product Details

Uniprot No.

P51884

Target Names

LUM

Alternative Names

Lumican (Keratan sulfate proteoglycan lumican) (KSPG lumican), LUM, LDC SLRR2D

Species Reactivity

Human

Immunogen

A synthesized peptide from human LUM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

17F4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Lumican is a small leucine-rich proteoglycan that plays essential roles in collagen fibrillogenesis, tissue organization, and extracellular matrix assembly. Beyond its structural functions, lumican has emerged as a significant player in wound healing, corneal transparency, and tumor microenvironment regulation, making it a compelling target for researchers investigating fibrosis, cancer progression, and connective tissue biology.

This recombinant monoclonal antibody, clone 17F4, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a sequence-defined rabbit IgG framework, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The antibody is raised against a synthetic peptide derived from human lumican and purified by affinity chromatography to ensure high specificity.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects lumican in Jurkat, HT-29, A431, and HeLa whole cell lysates at dilutions of 1:500–1:2000. The observed band size of 38–58 kDa, compared to the predicted 38 kDa molecular weight, reflects the extensive glycosylation characteristic of this keratan sulfate proteoglycan. Immunofluorescence staining of HeLa cells reveals clear cytoplasmic and extracellular matrix localization, while flow cytometry analysis confirms reliable detection in fixed and permeabilized cell populations.

This versatility across western blot, immunofluorescence, flow cytometry, and ELISA applications provides researchers with flexibility to characterize lumican expression through complementary approaches. Whether investigating stromal-epithelial interactions in cancer models, studying corneal pathology, or exploring fibrotic disease mechanisms, this antibody delivers the consistent performance needed for meaningful experimental insights.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA560532A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg), HT-29 whole cell lysate(30µg), A431 whole cell lysate(30µg), Hela whole cell lysate(30µg) All lanes: Lumican antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 38 kDa Observed band size: 38-58 kDa Exposure time：10s Immunofluorescence staining of Hela cell with CSB-RA560532A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA560532A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

LUM Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background