LY6E Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA619076MA1HU
Size	US$210
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Image	Immunofluorescence staining of A549 cell with CSB-RA619076MA1HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). The Binding Activity of Human LY6E with Anti-LY6E recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human LY6E protein (CSB-MP619076HU) at 2 μg/mL can bind Anti-LY6E recombinant antibody. The EC₅₀ is 2.483-3.282 ng/mL.
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Product Details
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Target Background

Product Details

Uniprot No.

Q16553

Target Names

LY6E

Alternative Names

LY6E antibody; 9804 antibody; RIGE antibody; SCA2 antibody; TSA1 antibody; Lymphocyte antigen 6E antibody; Ly-6E antibody; Retinoic acid-induced gene E protein antibody; RIG-E antibody; Stem cell antigen 2 antibody; SCA-2 antibody; Thymic shared antigen 1 antibody; TSA-1 antibody

Species Reactivity

Human

Immunogen

Recombinant Human LY6E protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

mIgG2a

Clone No.

29C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IF

Recommended Dilution

Application	Recommended Dilution
IF	1:50-1:200

Protocols

ELISA Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

LY6E, also known as stem cell antigen 2 or retinoic acid-induced gene E protein, is a GPI-anchored cell surface protein belonging to the Ly-6 superfamily. This interferon-stimulated gene plays multifaceted roles in immune regulation, viral entry modulation, and cancer biology. LY6E has garnered significant attention for its involvement in both promoting and restricting viral infections depending on the pathogen, while also serving as a marker in stem cell research and tumor immunology studies.

This recombinant monoclonal antibody, clone 29C11, offers the reproducibility and consistency that demanding research applications require. Generated against recombinant human LY6E protein and produced using recombinant technology, this antibody provides sequence-defined specificity that eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The mouse IgG2a isotype and affinity-chromatography purification ensure reliable performance across experiments.

Validation studies demonstrate robust performance in immunofluorescence applications, where staining of A549 human lung carcinoma cells at dilutions of 1:50 to 1:200 reveals clear membrane and cytoplasmic localization patterns consistent with LY6E's known GPI-anchored distribution. Functional ELISA testing confirms high-affinity binding to immobilized human LY6E protein, with EC50 values in the low nanogram per milliliter range, indicating excellent sensitivity for detection-based assays.

The liquid format in glycerol-containing PBS buffer supports straightforward integration into existing protocols while maintaining antibody stability during storage at -20°C or -80°C. Researchers investigating interferon signaling pathways, viral host-factor interactions, cancer immunology, or stem cell biology will find this antibody a dependable tool for characterizing LY6E expression and function in human cell systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

GPI-anchored cell surface protein that regulates T-lymphocytes proliferation, differentiation, and activation. Regulates the T-cell receptor (TCR) signaling by interacting with component CD3Z/CD247 at the plasma membrane, leading to CD3Z/CD247 phosphorylation modulation. Restricts the entry of human coronaviruses, including SARS-CoV, MERS-CoV and SARS-CoV-2, by interfering with spike protein-mediated membrane fusion. Plays also an essential role in placenta formation by acting as the main receptor for syncytin-A (SynA). Therefore, participates in the normal fusion of syncytiotrophoblast layer I (SynT-I) and in the proper morphogenesis of both fetal and maternal vasculatures within the placenta. May also act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity.; (Microbial infection) Promotes entry, likely through an enhanced virus-cell fusion process, of various viruses including HIV-1, West Nile virus, dengue virus and Zika virus. In contrast, the paramyxovirus PIV5, which enters at the plasma membrane, does not require LY6E. Mechanistically, adopts a microtubule-like organization upon viral infection and enhances viral uncoating after endosomal escape.

Gene References into Functions

LY6E belongs to a growing class of interferon-inducible factors that broadly enhance viral infectivity in an interferon-independent manner. PMID: 30190477
LY6E knockdown by targeted-siRNA inhibited gastric cancer cell survival and proliferation and induced G1-S cell cycle arrest and apoptosis in gastric cancer cells PMID: 29448250
High LY6E expression is associated with breast cancer. PMID: 27589564
LY6E overexpression is associated with Breast Cancer Progression, Immune Escape, and Drug Resistance. PMID: 27197181
LY6E is a positive modulator of HIV-1 infection in primary human peripheral blood mononuclear cells, immortalized CD4+ T lymphoid cells, and macrophages. HIV Long Terminal Repeat-driven HIV-1 gene expression is also enhanced by LY6E, suggesting additional roles of LY6E in HIV-1 replication. PMID: 28130445
LY6E level of gene expression may serve as good biomarkers for systemic lupus erythematosus diagnosis. PMID: 25344775
the LY6E pathway in monocytes represents one of negative feedback mechanisms that counterbalance monocyte activation which may serve as a potential target for immune intervention. PMID: 25225669
This study suggested that a mutation in SCA2 or SCA3/MJD may be one of the genetic causes of Parkinson's disease in china. PMID: 19672991
Increased expression of the type I interferon-inducible gene, lymphocyte antigen 6 complex locus E, in peripheral blood cells is predictive of lupus activity in a large cohort of Chinese lupus patients. PMID: 18755862
Two SCA3 and one SCA2 cases have been identified which show autosomal dominant inheritance in Parkinson disease. PMID: 18990604

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity

Widely expressed, predominantly in liver, kidney, ovary, spleen and peripheral blood Leukocytes.

Database Links

HGNC: 6727

OMIM: 601384

KEGG: hsa:4061

STRING: 9606.ENSP00000292494

UniGene: Hs.521903

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Related pathways

Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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