Code | CSB-RA015007A1HU |
Size | US$210 |
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The generation of the MS4A1 recombinant monoclonal antibody involves a meticulous step-by-step process to ensure its exceptional quality and specificity. It begins with the isolation of B cells from an immunized animal, where the recombinant human MS4A1 protein is used as the immunogen. Total RNA is extracted from these B cells and converted into cDNA through reverse transcription. The MS4A1 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently introduced into host cells via transfection to facilitate the production of the MS4A1 recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. ELISA is conducted to validate the antibody's specificity and functionality in detecting human MS4A1 protein. This stringent production process ensures the generation of a reliable and effective MS4A1 recombinant monoclonal antibody, essential for a wide range of MS4A1-related research.
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