Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
MS4A1; CD20; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD antigen CD20
Species
Homo sapiens (Human)
Expression Region
141-297aa
Target Protein Sequence
IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Yeast-expressed Recombinant partial-length Human B-lymphocyte antigen CD20 (MS4A1) contains the region of 141-297aa of human MS4A1. It was fused with a 6xHis-tag at the N-terminus. The purity of this protein is over 90% as measured by SDS-PAGE. On the reducing SDS-PAGE gel, this MS4A1 protein migrated to the molecular weight band of about 22 kDa. This observed molecular mass is slightly higher than the calculated one (20.0 kDa) due to glycosylation. Except for antibody synthesis, this recombinant MS4A1 protein also may be used in the studies of MS4A1-associated immunology.
CD20, encoded by the gene MS4A1, is a B cell differentiation antigen expressed from the pre-B to mature B cell stage, but lost upon terminal
differentiation into plasma cells. CD20 is physiologically directly required for efficient BCR signaling in B cells. It normally exists as homodimeric and homotetrameric oligomers on the cell surface and combines with other cell-surface and cytoplasmic proteins, contributing to the signal transduction. Currently, CD20 antigen has been considered as an ideal target for immunotherapy of B cell malignancies and B cell-mediated autoimmune diseases.