Code | CSB-RA010429A16me1HU |
Size | US$210 |
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Application | Recommended Dilution |
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IF | 1:30-1:200 |
The generation of the mono-methyl-Histone H4 (K16) recombinant monoclonal antibody entails a multi-step process that initiates with the cloning of the genes responsible for encoding the HIST1H4A antibody. These genes, encompassing both the heavy and light chains, are integrated into expression vectors designed for optimal performance. The next phase involves the introduction of the expression vectors into host cells through transfection, where the host cells are subsequently tasked with the production and secretion of the antibody. To ensure its purity and efficacy, the antibody then undergoes a meticulous purification procedure utilizing affinity chromatography. Once purified, the antibody is put through ELISA and IF tests, ultimately enabling precise and reliable detection of the human and rat HIST1H4A proteins mono-methylated at K16.
Mono-methylation of HIST1H4A at K16 primarily functions in chromatin compaction, transcriptional repression, DNA repair, cellular identity, long-range chromatin interactions, and epigenetic memory, and has implications in various diseases. It is a crucial epigenetic modification that helps regulate gene expression and chromatin structure.
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