Product Details

Uniprot No.

Target Names

Alternative Names

Cytoplasmic protein NCK1 antibody; Melanoma Nck protein antibody; MGC12668 antibody; NCK 1 antibody; NCK adaptor protein 1 antibody; NCK alpha antibody; NCK antibody; NCK tyrosine kinase antibody; Nck-1 antibody; NCK1 antibody; NCK1_HUMAN antibody; NCKalpha antibody; Non catalytic region of tyrosine kinase antibody; SH2/SH3 adaptor protein NCK alpha antibody; SH2/SH3 adaptor protein NCK-alpha antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human NCK1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

2H9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NCK1 functions as a critical adaptor protein that bridges receptor tyrosine kinases to downstream signaling cascades, playing essential roles in cytoskeletal reorganization, cell migration, and T cell receptor signaling. Through its SH2 and SH3 domains, NCK1 coordinates protein-protein interactions that translate extracellular signals into cellular responses, making it a key node in understanding signal transduction networks relevant to cancer biology, immunology, and developmental research.

This recombinant monoclonal antibody, generated from clone 2H9, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly in rabbit host, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived reagents. Affinity chromatography purification ensures high specificity for your target.

Validation studies demonstrate robust performance across multiple applications. In Western blot analysis, the antibody detects NCK1 in a diverse panel of human cell lines including HeLa, K562, A549, U-251MG, and PC-3, as well as mouse brain tissue lysate, confirming cross-species reactivity between human and mouse samples. The observed band at 47 kDa runs slightly higher than the 43 kDa predicted molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation events that regulate NCK1 activity. Flow cytometry validation in HeLa cells confirms utility for intracellular detection of NCK1 in fixed and permeabilized samples.

Whether investigating receptor tyrosine kinase signaling pathways, studying cytoskeletal dynamics, or exploring adaptor protein networks in cancer or immune cell biology, this antibody provides a reliable tool for characterizing NCK1 expression and localization across your experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.

Gene References into Functions

There was no difference in the Nck1 mRNA expression between the SLE patients and the healthy subjects. PMID: 29911835
this study highlights an important role for Nck1 in fine-tuning IRE1alpha expression and signaling that regulate PTP1B expression and subsequent activation of the PI3K-Akt pathway in HepG2 cells. PMID: 28455143
These findings suggest a novel STAT3 to NCK1 to PAK1/ERK signaling mechanism that is potentially critical for colorectal cancer metastasis and angiogenesis. PMID: 28455144
Authors provide evidence that Nck is an upstream regulator of RhoA-dependent, MMP14-mediated breast carcinoma cell invasion. PMID: 28954862
Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID: 27033705
Results demonstrate that Nck acts as an important hub integrating angiogenic cues with cytoskeletal changes that enable endothelial apical-basal polarization and lumen formation. PMID: 26157164
the binding of Nck to the TCR requires partial phosphorylation of CD3epsilon, as it is based on two cooperating interactions. PMID: 26590318
Nck was the most potent activator of N-WASP-driven actin assembly. PMID: 26554011
TSAD binds to and co-localizes with Nck. Expression of TSAD increases both Nck-Lck and Nck-SLP-76 interaction in T cells. PMID: 26163016
these data identify Nck as an important mediator of oxidative stress-induced inflammation and a potential therapeutic target for ischemia/reperfusion injury. PMID: 25714462
Nck1 depletion induces activation of the PI3K/Akt pathway by attenuating PTP1B protein expression PMID: 25398386
Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID: 25482634
biochemical reconstitution on supported lipid bilayers of protein clusters containing the adhesion receptor Nephrin and its cytoplasmic partners, Nck and N-WASP, is reported. PMID: 25321392
SDF1alpha-induced interaction of the adapter proteins Nck and HS1 facilitates actin polymerization and migration in T cells. PMID: 25359136
These results identify a new domain of CE that is specific to its function in cytoplasmic capping, and a new role for Nck1 in regulating gene expression through its role as the scaffold for assembly of the cytoplasmic capping complex. PMID: 25137142
Nck ubiquitylation might serve as a mechanism controlling Nck-mediated effector functions during cellular activation PMID: 25218436
the present study characterized a novel Nck-1-ELMO1 interaction and defined a new role for Nck-1 in regulating Rac1 activity. PMID: 24928514
Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID: 24287595
Our observations suggest that ITSN1 is an important general regulator of Cdc42-, Nck- and N-WASP-dependent actin polymerisation PMID: 24284073
we have optimized the GST-Nck1-SH2 pull-down procedure to obtain tyrosine-phosphorylated proteins in tumor tissues PMID: 23426619
Depletion of the adaptor protein NCK increases UV-induced p53 phosphorylation and promotes apoptosis. PMID: 24086708
ADAP and Nck adapter proteins cooperatively facilitate T cell adhesion to the LFA-1 ligand ICAM-1. PMID: 24769494
that Nck recruitment to the TCR is fundamental to mount an efficient T cell response in vivo, and that the Nck-CD3epsilon interaction may represent a target for pharmacological modulation of the immune response. PMID: 24470497
OSU-03012 and lapatinib co-treatment is an effective combination therapy, which functions to enhance cell killing through the Nck1/eIF2 complex PMID: 23706161
The study identifies Nck as a key coordinator of cytoskeletal changes that enable cell polarization and directional migration, which are crucial processes in development and disease. PMID: 23444376
NCK1 plays a pivotal role in integrating endoplasmic reticulum stress signals on cancer cell survival. PMID: 23448571
The results suggest that MNT, via interaction with Nck1, inhibits hepatoma cell migration. PMID: 22964333
LRFN4 complexed with 14-3-3s and NCK1 to mediate elongation in monocytic cells via Rac-1-mediated actin cytoskeleton reorganization PMID: 22677168
both T cell activation and the association between SLP-76 and Nck. After T cell receptor stimulation, SLP-76 was phosphorylated, which enabled the binding of Nck. PMID: 22534133
The results indicate that the density of Nck molecules in aggregates is a critical determinant of actin polymerization. PMID: 22613834
the negative loop on p38 is mediated by c-ABL phosphorylation at tyrosine 105 of the adaptor protein NCK1, while the phosphorylation at tyrosine 209 of GRB2 down-modulates ERK1/2 and JNKs signaling. PMID: 22327338
Decreased Nck1 protein in Jurkat T cells resulted in an impairment of TCR-CD3-mediated activation involving a defective Erk phosphorylation pathway. PMID: 22132889
Adapter protein Nck sequesters PAK1 in the cytoplasm and that coexpression of both PAK1 and Nck inhibits the amplifying effect of PRL-induced PAK1 on cyclin D1 promoter activity. PMID: 21719533
Nck1 association with RasGAP depends on cell adhesion to the substrate. PMID: 21664272
Studies reveal for the first time that the adaptor protein Dock/Nck attenuates insulin signalling by recruiting PTP61F/PTP1B to its substrate, the IR. PMID: 21707536
Nck1 is an adaptor protein composed of 3 N-terminal SH3 domains followed by a unique Cterminal SH2 domain. It plays a role in cell migration, cell adhesion, actin polymerization, stress responses and cell survival. It is implicated in melanoma. Review. PMID: 21880263
Nck1 activates RasGAP by direct binding in the substrate-attached but not in the suspended cells. PMID: 21664272
a functional cooperation between Nck and ADAP in stabilizing the recruitment of WASp to SLP76 regulates actin rearrangement. PMID: 21536650
Nck1 specifically localizes to invadopodia, but not to podosomes formed in macrophages or degradative structures formed in Src-transformed fibroblasts and PMA-stimulated endothelial cells. PMID: 20850195
Mass spectrometry analysis revealed a group of Nck1 SH2 domain-binding proteins that were differentially expressed in HCC. One of these proteins, dermcidin (DCD). PMID: 21397687
Results define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex, which is crucial for regulation of the actin machinery after T-cell activation. PMID: 20562827
The novel ponsin isoform and its interaction with Nck1/2 provide exciting insight into the convergence of signalling pathways at the costameres, and its crucial role for skeletal muscle differentiation and re-generation. PMID: 20129698
The results show that Bcr-Abl regulates the actin cytoskeleton and non-apoptotic membrane blebbing via a GADS/Slp-76/Nck1 adaptor protein pathway. PMID: 20079431
Modulation of protein translation by Nck-1. PMID: 11959995
Grb2 and Nck act cooperatively to promote actin-based motility of vaccinia virus PMID: 12007418
The association of NCK with Wiskott-Aldrich syndrome protein is associated with cell migration in stromal cell-derived factor-1alpha-stimulated Jurkat cells PMID: 12135674
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck: we propose that Rac1 and Nck cause dissociation of the WAVE1 complex, which releases active WAVE1-HSPC300 and leads to actin nucleation. PMID: 12181570
the interaction of the betaPIX.WASP.SPIN90 complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERK activation PMID: 14559906
Data show that clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly. PMID: 14757753
Nck and Crk mediate distinct VEGF-induced signaling pathways that serve overlapping functions in cell migration. PMID: 15051508

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Subcellular Location

Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.

Database Links

HGNC: 7664

OMIM: 600508

KEGG: hsa:4690

STRING: 9606.ENSP00000288986

UniGene: Hs.126889

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Code	CSB-RA097617A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HeLa whole cell lysate(30µg), K562 whole cell lysate(30µg), A549 whole cell lysate, U-251MG whole cell lysate(30µg), PC-3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg) All lanes: NCK1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43 kDa Observed band size: 47 kDa Exposure time: 1min Overlay Peak curve showing Hela cells stained with CSB-RA097617A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NCK1 Recombinant Monoclonal Antibody

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Target Background