Product Details

Uniprot No.

P21589

Target Names

NT5E

Alternative Names

5'-nucleotidase (5'-NT) (EC 3.1.3.5) (Ecto-5'-nucleotidase) (CD antigen CD73), NT5E, NT5 NTE

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CD73

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6F6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NT5E, also known as CD73 or ecto-5'-nucleotidase, is a GPI-anchored cell surface enzyme that catalyzes the dephosphorylation of extracellular AMP to adenosine. This enzymatic activity positions NT5E as a critical regulator of purinergic signaling, with significant implications for cardiovascular research, immune modulation, and tumor microenvironment studies. The adenosine generated by NT5E influences vascular tone, inflammatory responses, and has emerged as an important factor in cancer immunology, where its expression often correlates with immunosuppressive tumor phenotypes.

This recombinant monoclonal antibody, clone 6F6, offers the reproducibility and consistency that demanding research protocols require. Because recombinant antibodies are produced from defined genetic sequences rather than hybridoma supernatants, you can expect reliable performance across experiments and between lot numbers, eliminating a common source of variability in longitudinal studies or multi-site collaborations. The rabbit IgG format provides excellent signal-to-noise characteristics, and affinity chromatography purification ensures high specificity for your target.

Validation testing has demonstrated robust performance in immunohistochemistry applications using human tissue samples. The antibody has been successfully tested in paraffin-embedded human endometrial cancer and colon cancer tissues at 1:100 dilution using citrate buffer antigen retrieval, producing clear DAB visualization on automated Leica Bond systems. These validated protocols in clinically relevant cancer tissues provide a solid foundation for researchers investigating NT5E expression patterns in oncology contexts. The antibody is also suitable for ELISA applications, with recommended IHC dilutions ranging from 1:50 to 1:200 to accommodate optimization across different tissue types and detection systems.

For cardiovascular and cancer researchers studying purinergic signaling pathways, this antibody provides a dependable tool for characterizing NT5E expression in human specimens.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA978310A0HU
Size	US$210
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Image	IHC image of CSB-RA978310A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA978310A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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NT5E Recombinant Monoclonal Antibody

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