CD73 (also known as Ecto-5'-Nucleotidase), a cell surface enzyme encoded by the NT5E gene, is a major enzyme catalyzing the formation of extracellular adenosine from AMP. The gene for human CD73 is located on chromosome 6q14-921. CD73 is conservative in different species. For example, the cDNA sequence of human CD73 has 86% homology with that of mouse.
CD73 is widely expressed on the surface of human tissue cells, including liver cells, fibroblasts, endothelial cells, lymphocytes and glial cells, and proximal tubular epithelial cells. CD73 is a metabolic immune checkpoint that coordinates the critical homeostasis of extracellular adenosine levels. It undergoes homo-dimerization mediated by N-linked glycosylation and disulfide bonds, and is anchored to the cell membrane surface through GPI. It is one of the rate-limiting enzymes in the extracellular purine metabolism pathway.
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● Recombinant Human 5'-nucleotidase(NT5E) (Active) CSB-MP723415HU
Validated Data
Greater than 95% as determined by SDS-PAGE.
Immobilized CD73 at 2 μg/ml can bind Anti- CD73 Rabbit Monoclonal Antibody (CSB-RA978310A0HU), the EC50 is 3.212-4.525 ng/ml.
● NT5E Recombinant Monoclonal Antibody CSB-RA978310A0HU
Validated Data
IHC image of CSB-RA978310A0HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA978310A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
The following NT5E reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
NT5E Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA420339 | NT5E Antibody |
Human | WB, ELISA |
CSB-PA564996 | NT5E Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA823804 | NT5E Antibody |
Human,Mouse,Rat | ELISA,IHC |
CSB-PA10529A0Rb | NT5E Antibody |
Human | ELISA, WB, IHC |
CSB-PA10529B0Rb | NT5E Antibody, HRP conjugated |
Human | ELISA |
CSB-PA10529C0Rb | NT5E Antibody, FITC conjugated |
Human | |
CSB-PA10529D0Rb | NT5E Antibody, Biotin conjugated |
Human | ELISA |
CSB-RA978310A0HU | NT5E Recombinant Monoclonal Antibody |
Human | ELISA, IHC |
NT5E Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP723415MO CSB-EP723415MO CSB-BP723415MO CSB-MP723415MO CSB-EP723415MO-B |
Recombinant Mouse 5'-nucleotidase (Nt5e) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-AP005101MO | Recombinant Mouse 5'-nucleotidase (Nt5e), partial (Active) |
Mammalian cell |
NT5E Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-EP723415HU1 | Recombinant Human 5'-nucleotidase (NT5E), partial |
E.coli |
CSB-EP723415HU1b1 | Recombinant Human 5'-nucleotidase (NT5E), partial |
E.coli |
CSB-AP005071HU | Recombinant Human 5'-nucleotidase (NT5E), partial (Active) |
Mammalian cell |
CSB-MP723415HU | Recombinant Human 5'-nucleotidase (NT5E) (Active) |
Mammalian cell |
NT5E ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E04682m | Mouse 5-Nucleotidase,5-NT ELISA Kit |
serum, plasma, tissue homogenates | 0.031 mU/mL |
NT5E ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E14099h | Human cluster of differentiation 73(CD73) ELISA Kit |
serum, plasma, tissue homogenates | 0.78 ng/mL |
NT5E ELISA Kit for Rattus norvegicus (Rat)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E14100r | Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit |
serum, plasma, tissue homogenates, cell culture supernates | 0.156 mIU/mL |