NT5E Research Reagents

CD73 (also known as Ecto-5'-Nucleotidase), a cell surface enzyme encoded by the NT5E gene, is a major enzyme catalyzing the formation of extracellular adenosine from AMP. The gene for human CD73 is located on chromosome 6q14-921. CD73 is conservative in different species. For example, the cDNA sequence of human CD73 has 86% homology with that of mouse.

CD73 is widely expressed on the surface of human tissue cells, including liver cells, fibroblasts, endothelial cells, lymphocytes and glial cells, and proximal tubular epithelial cells. CD73 is a metabolic immune checkpoint that coordinates the critical homeostasis of extracellular adenosine levels. It undergoes homo-dimerization mediated by N-linked glycosylation and disulfide bonds, and is anchored to the cell membrane surface through GPI. It is one of the rate-limiting enzymes in the extracellular purine metabolism pathway.

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Popular Product

Recombinant Human 5'-nucleotidase(NT5E) (Active) (CSB-MP723415HU)

Validated Data

Purity determined
by SDS-PAGE

Greater than 95% as determined by SDS-PAGE.

The binding activity of antigen-antibody validated by functional ELISA

Immobilized CD73 at 2 μg/ml can bind Anti- CD73 Rabbit Monoclonal Antibody (CSB-RA978310A0HU), the EC₅₀ is 3.212-4.525 ng/ml.