Code | CSB-RA581794A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
FC | 1:20-1:200 |
CUSABIO employed an immunization strategy by inoculating a rabbit with a human PARP1-derived peptide to stimulate an immune response. Splenocytes were subsequently obtained from the immunized rabbit, and RNA was extracted from these cells. Through reverse transcription, the extracted RNA was converted into complementary DNA (cDNA). The PARP1 antibody gene was then extended using degenerate primers. PCR amplification facilitated the generation of PARP1 antibody gene fragments. After purification, the fragments were cloned into an expression vector and transfected into a host system for efficient antibody production. The resulting PARP1 recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. Multiple applications including ELISA, WB, IHC, IF, and FC were conducted to validate the binding specificity and affinity of the PARP1 recombinant monoclonal antibody. Remarkably, this antibody exhibits specific recognition of the human PARP1 protein, showcasing its potential for targeted research and therapeutic applications.
Applications : Western Blot (WB)
Sample type: Human cancer cell line
Sample dilution: 1:1000
Review: clean specific band on western blot, low background and very easy to use
By Anonymous