Code | CSB-RA213932A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The PDGFRB recombinant monoclonal antibody is generated through protein and DNA recombinant technologies. The process starts with the immunization of mice with a synthesized peptide obtained from human PDGFRB protein. The spleen cells are harvested from the immunized mice after a specific period under sterile conditions, and their total RNA is extracted. cDNA synthesized by RNA reverse transcription serves as the template for PCR amplification of the PDGFRB antibody gene. Subsequently, the gene is inserted into a vector and then transfected into host cells for cultivation. The PDGFRB recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography and verified for its accuracy. It is highly reliable and applicable in ELISA and IHC experiments for detecting human PDGFRB protein.
The PDGFRB protein is a receptor tyrosine kinase that is involved in various cellular processes, including cell proliferation, differentiation, and survival. When PDGFRB is activated by binding to its ligand PDGF, it undergoes dimerization, autophosphorylation, and activation of downstream signaling pathways, such as the PI3K/AKT pathway, JAK/STAT pathway, and RAS/MAPK pathway, which regulate cell differentiation, proliferation, and survival. Dysregulation of PDGFRB signaling has been implicated in the pathogenesis of several diseases, including cancer, fibrosis, and atherosclerosis.
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