PTBP2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA201933A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in: SH-SY5Y whole cell lysate(30µg), U251 whole cell lysate(30µg), LO2 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: PTBP2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time：60s IHC image of CSB-RA201933A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA201933A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of SH-SY5Y cell with CSB-RA201933A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA201933A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9UKA9

Target Names

PTBP2

Alternative Names

brPTB antibody; FLJ34897 antibody; MIBP antibody; Neural polypyrimidine tract binding protein antibody; Neural polypyrimidine tract-binding protein antibody; Neurally enriched homolog of PTB antibody; Neurally-enriched homolog of PTB antibody; nPTB 5 antibody; nPTB 6 antibody; nPTB 7 antibody; nPTB 8 antibody; nPTB antibody; nPTB5 antibody; nPTB6 antibody; nPTB7 antibody; nPTB8 antibody; Polypyrimidine tract binding protein 2 antibody; Polypyrimidine tract-binding protein 2 antibody; PTB antibody; PTB like antibody; PTB like protein antibody; PTB-like protein antibody; PTBLP antibody; PTBP 2 antibody; Ptbp2 antibody; PTBP2_HUMAN antibody; Splicing regulator antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human PTBP2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PTBP2, also known as neural polypyrimidine tract-binding protein, serves as a critical RNA-binding protein that regulates alternative splicing programs essential for neuronal differentiation and brain development. This splicing regulator plays a particularly important role in the transition from neural progenitor cells to mature neurons, making it a valuable target for researchers investigating neurodevelopmental processes, neurological disorders, and the mechanisms underlying cell fate determination.

This recombinant monoclonal antibody, clone 3E1, offers the reproducibility and consistency that demanding research applications require. Generated through recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for PTBP2 detection across your experimental workflow.

Validation data demonstrates robust performance across multiple applications. Western blot analysis confirms detection of PTBP2 at the expected 57 kDa molecular weight in human neuroblastoma cells (SH-SY5Y), human glioblastoma cells (U251), human hepatocytes (LO2), as well as mouse and rat brain tissue lysates, confirming cross-species reactivity across human, mouse, and rat samples. Immunohistochemistry staining has been validated in paraffin-embedded human testis and liver cancer tissues using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence analysis in SH-SY5Y cells reveals clear nuclear staining patterns consistent with PTBP2's role in pre-mRNA processing. Flow cytometry validation further demonstrates utility for quantitative single-cell analysis of PTBP2 expression levels.

This antibody supports investigations into epigenetics and nuclear signaling pathways, particularly studies examining splicing regulation during neural development, cancer biology, and tissue-specific gene expression programs.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.; Reduced affinity for RNA.

Gene References into Functions

It has been established that PTBP1 and PTBP2 are members of a family of cryptic exon repressors. PMID: 27681424
PTBP1 and PTBP2 impaired autoregulation of SRSF3 in oral squamous cell carcinoma cancer cells. PMID: 26416554
Data show that polypyrimidine tract-binding proteins nPTB and ROD1 interact with mitochondrial tRNA(Thr) in the cytoplasm outside of mitochondria. PMID: 26657638
In T98G glioma cells, the level of sumoylated PTBP2 was reduced compared to that of normal brain cells. Overall, this study shows that PTBP2 is posttranslationally modified by SUMO1. PMID: 24286314
MALAT1 binds to SFPQ releasing PTBP2 from the SFPQ/PTBP2 complex, the increased SFPQ-detached PTBP2 promotes cell proliferation and migration in colorectal cancer. PMID: 25025966
Defining the multifunctional roles of PTB will contribute to the understanding of key regulatory events in gene expression. PMID: 24264039
Changes in miR-223/PTBP2 pathway could contribute of abnormal splicing in chronic myeloid leukemia. PMID: 23174904
Regulation of the mutually exclusive exons 8a and 8 in the CaV1.2 calcium channel transcript by polypyrimidine tract-binding protein. PMID: 21282112
present fluorescence, NMR, and in vivo splicing data in support of a role of PTB in inducing RNA loops. We show that the RNA recognition motifs (RRMs) 3 and 4 of PTB can bind two distant pyrimidine tracts and bring their 5' and 3' ends in close proximity PMID: 20160105
The nPTB proximal promoter, although rich in G+C content and presenting putative binding sites for the transcription factors Sp1, NF-1, NF-kB and Oct-1, lacks a typical TATA box. PMID: 16002244
Study shows that PTB can function as an anti-repressor molecule to counteract the splicing inhibitory activity of SRp30c. PMID: 17548433

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Subcellular Location

Nucleus.

Tissue Specificity

Mainly expressed in brain although also detected in other tissues like heart and skeletal muscle. Isoform 1 and isoform 2 are specifically expressed in neuronal tissues. Isoform 3 and isoform 4 are expressed in non-neuronal tissues. Isoform 5 and isoform

Database Links

HGNC: 17662

OMIM: 608449

KEGG: hsa:58155

STRING: 9606.ENSP00000412788

UniGene: Hs.596061

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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