PVRL2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA582013A0HU
Size	US$210
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Image	IHC image of CSB-RA582013A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA582013A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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Product Details
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Target Background

Product Details

Uniprot No.

Q92692

Target Names

PVRL2

Alternative Names

CD 112 antibody; CD112 antibody; CD112 antigen antibody; Herpes virus entry mediator B antibody; Herpes virus entry protein B antibody; Herpesvirus entry mediator B antibody; Herpesvirus entry protein B antibody; Hve B antibody; HveB antibody; Nectin-2 antibody; Nectin2 antibody; Poliovirus receptor like 2 antibody; Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody; Poliovirus receptor related 2 antibody; Poliovirus receptor related protein 2 antibody; Poliovirus receptor-related protein 2 antibody; PRR 2 antibody; PRR2 antibody; PVRL 2 antibody; PVRL2 antibody; PVRL2_HUMAN antibody; PVRR 2 antibody; PVRR2 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PVRL2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

16F10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PVRL2, also known as Nectin-2 or CD112, is a cell adhesion molecule belonging to the immunoglobulin superfamily that plays multifaceted roles in cell-cell interactions, viral entry, and immune regulation. As a ligand for TIGIT and CD226 (DNAM-1), PVRL2 has emerged as a significant player in immunology research, particularly in studies examining immune checkpoint pathways and tumor microenvironment dynamics. Its expression patterns across various tissues make it a valuable marker for understanding both normal physiology and disease states.

This recombinant monoclonal antibody, clone 16F10, offers the reproducibility and consistency that demanding research protocols require. Produced using recombinant technology with a sequence-defined approach, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for human PVRL2, while affinity chromatography purification delivers a clean reagent suitable for sensitive detection methods.

Validation in immunohistochemistry demonstrates reliable performance across distinct human tissue contexts. Testing in paraffin-embedded human testis tissue and human prostate cancer specimens at 1:100 dilution using citrate buffer antigen retrieval confirms the antibody's utility for both normal tissue characterization and oncology-focused investigations. The validated IHC dilution range of 1:50-1:200 provides flexibility for optimization across different sample types and detection systems.

For researchers investigating immune checkpoint biology, cell adhesion mechanisms, or tumor immunology, this antibody provides a dependable tool for characterizing PVRL2 expression in human tissues. Its compatibility with standard IHC workflows on automated platforms like the Leica Bond system supports integration into established laboratory protocols.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.; (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Gene References into Functions

Nectin-2 mutation in men with severe teratospermia PMID: 28689229
In the infection with 3 MLD50 (50 % mouse lethal dose), effective resistance was not observed in transgenic mice expressing nectin-2Ig. PMID: 28671524
Our data provide important structural and biochemical determinants responsible for the recognition of nectin-2 by TIGIT. PMID: 27978489
Chromosomal breakpoints involved the PVRR2 gene in 19q31 is associated with Diffuse Large B-Cell Lymphomas. PMID: 27356265
energetic basis for the TIGIT/nectin-2 interaction and revealed that an "aromatic key" of nectin-2 is critical for this interaction, whereas variations in the lock were tolerated. PMID: 28515320
PVRL2 is a plasma cholesterol-responsive gene acting at endothelial sites of vascular inflammation to regulate transendothelial migration of leukocytes. PMID: 28062492
Serum levels of nectin-2 may have diagnostic roles for colorectal cancer patients. PMID: 26184725
Soluble form of nectin-2 is required for exerting the resistance against HSV-2 infection. PMID: 26982466
PVRL2, TOMM40 and APOE might be associated with human longevity. PMID: 24924924
Nectin-3 trans-interacts with Nectin-2 to promote lymphocyte and monocyte extravasation. PMID: 24116228
Chorionic gonadotropin induces vascular endothelial growth factor (VEGFA)-dependent downregulation of nectin 2, which increases the endothelial permeability in the coculture system. PMID: 23465821
Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. PMID: 22927415
a possible etiologic role of PRR2 in nonsyndromic cleft lip with or without cleft palate PMID: 20662561
The CD112 is highly expressed in colon carcinoma tissues and cell lines. PMID: 20617580
The authors now show that human cytomegalovirus targets CD112 for proteasome-mediated degradation by 48 h post-infection, thus removing both activating ligands for DNAM-1 from the cell surface during productive infection. PMID: 20410314
differences in the N termini of herpes simplex virus type 1 and 2 gDs that influence functional interactions with the human entry receptor Nectin-2 PMID: 12915581
There is an allelic association of sequence variation in PVRL2 and the severity of multiple sclerosis. PMID: 16738668
CD226/CD112 (DNAM-1/Nectin-2) mast cell stimulation has a role in the allergic process PMID: 16831868
identified PVRL2 as a new recurrent partner gene of the TRA@ locus in peripheral T-cell lymphoma PMID: 17696193
Crystallization and preliminary X-ray analysis of the V domain of human nectin-2 are reported. PMID: 19478445
Regions of nectin-2 protein important for herpesvirus entry activity and homotypic nectin-nectin interactions are overlapping but not identical. PMID: 12438620

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Nectin family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9707

OMIM: 600798

KEGG: hsa:5819

STRING: 9606.ENSP00000252483

UniGene: Hs.655455

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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