Product Details

Uniprot No.

Target Names

Alternative Names

CD 112 antibody; CD112 antibody; CD112 antigen antibody; Herpes virus entry mediator B antibody; Herpes virus entry protein B antibody; Herpesvirus entry mediator B antibody; Herpesvirus entry protein B antibody; Hve B antibody; HveB antibody; Nectin-2 antibody; Nectin2 antibody; Poliovirus receptor like 2 antibody; Poliovirus receptor related 2 (herpesvirus entry mediator B) antibody; Poliovirus receptor related 2 antibody; Poliovirus receptor related protein 2 antibody; Poliovirus receptor-related protein 2 antibody; PRR 2 antibody; PRR2 antibody; PVRL 2 antibody; PVRL2 antibody; PVRL2_HUMAN antibody; PVRR 2 antibody; PVRR2 antibody

Species Reactivity

Macaca fascicularis

Immunogen

Recombinant Human NECTIN2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

6C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:5000-1:50000

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NECTIN2, also known as CD112 or poliovirus receptor-related protein 2, serves as a critical cell adhesion molecule and immune checkpoint regulator that has garnered significant attention in immunology research. This protein functions as an entry mediator for certain herpesviruses and plays essential roles in cell-cell adhesion at adherens junctions. Importantly, NECTIN2 interacts with immune receptors including TIGIT and CD226, positioning it as a key player in modulating T cell and NK cell responses—making it a compelling target for researchers investigating immune regulation and checkpoint biology.

This recombinant monoclonal antibody (clone 6C10) offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined genetic sequences rather than hybridoma supernatants, researchers benefit from lot-to-lot uniformity that supports reliable data across extended studies and multi-site collaborations. The human IgG1 isotype format and affinity-chromatography purification further ensure high purity suitable for sensitive detection methods.

Validation studies demonstrate robust binding activity against Macaca fascicularis NECTIN2 in functional ELISA, with EC50 values ranging from 1.011 to 1.193 ng/mL when tested against immobilized antigen at 2 μg/mL. This high-affinity binding supports reliable detection at working dilutions between 1:5000 and 1:50000, offering flexibility to optimize signal-to-background ratios for your specific experimental conditions.

For researchers exploring immune checkpoint mechanisms, viral entry pathways, or cell adhesion biology in non-human primate models, this antibody provides a sequence-defined tool with demonstrated performance in ELISA-based applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein.; (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Gene References into Functions

Nectin-2 mutation in men with severe teratospermia PMID: 28689229
In the infection with 3 MLD50 (50 % mouse lethal dose), effective resistance was not observed in transgenic mice expressing nectin-2Ig. PMID: 28671524
Our data provide important structural and biochemical determinants responsible for the recognition of nectin-2 by TIGIT. PMID: 27978489
Chromosomal breakpoints involved the PVRR2 gene in 19q31 is associated with Diffuse Large B-Cell Lymphomas. PMID: 27356265
energetic basis for the TIGIT/nectin-2 interaction and revealed that an "aromatic key" of nectin-2 is critical for this interaction, whereas variations in the lock were tolerated. PMID: 28515320
PVRL2 is a plasma cholesterol-responsive gene acting at endothelial sites of vascular inflammation to regulate transendothelial migration of leukocytes. PMID: 28062492
Serum levels of nectin-2 may have diagnostic roles for colorectal cancer patients. PMID: 26184725
Soluble form of nectin-2 is required for exerting the resistance against HSV-2 infection. PMID: 26982466
PVRL2, TOMM40 and APOE might be associated with human longevity. PMID: 24924924
Nectin-3 trans-interacts with Nectin-2 to promote lymphocyte and monocyte extravasation. PMID: 24116228
Chorionic gonadotropin induces vascular endothelial growth factor (VEGFA)-dependent downregulation of nectin 2, which increases the endothelial permeability in the coculture system. PMID: 23465821
Structure of Nectin-2 reveals determinants of homophilic and heterophilic interactions that control cell-cell adhesion. PMID: 22927415
a possible etiologic role of PRR2 in nonsyndromic cleft lip with or without cleft palate PMID: 20662561
The CD112 is highly expressed in colon carcinoma tissues and cell lines. PMID: 20617580
The authors now show that human cytomegalovirus targets CD112 for proteasome-mediated degradation by 48 h post-infection, thus removing both activating ligands for DNAM-1 from the cell surface during productive infection. PMID: 20410314
differences in the N termini of herpes simplex virus type 1 and 2 gDs that influence functional interactions with the human entry receptor Nectin-2 PMID: 12915581
There is an allelic association of sequence variation in PVRL2 and the severity of multiple sclerosis. PMID: 16738668
CD226/CD112 (DNAM-1/Nectin-2) mast cell stimulation has a role in the allergic process PMID: 16831868
identified PVRL2 as a new recurrent partner gene of the TRA@ locus in peripheral T-cell lymphoma PMID: 17696193
Crystallization and preliminary X-ray analysis of the V domain of human nectin-2 are reported. PMID: 19478445
Regions of nectin-2 protein important for herpesvirus entry activity and homotypic nectin-nectin interactions are overlapping but not identical. PMID: 12438620

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Nectin family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 9707

OMIM: 600798

KEGG: hsa:5819

STRING: 9606.ENSP00000252483

UniGene: Hs.655455

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA835690MA1HU
Size	$49.9
	Order now
Promotion
Image	The Binding Activity of Macaca fascicularis NECTIN2 with Anti-NECTIN2 recombinant antibody Activity: Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis NECTIN2(CSB-MP5776MOV) at 2 μg/mL can bind Anti-NECTIN2 recombinant antibody. The EC₅₀ is 1.011-1.193 ng/mL.
Have Questions?	Leave a Message or Start an on-line Chat

NECTIN2 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background