Phospho-GYS1 (S641) Antibody

Code CSB-RA010078A641phHU
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
    All lanes Phospho-GYS1 antibody at 1.12μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 85 KDa
    Observed band size: 85 KDa

  • Immunofluorescence staining of HepG2 cells with CSB-RA010078A641phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Uniprot No. P13807
Target Names GYS1
Alternative Names Glycogen [starch] synthase antibody; Glycogen synthase 1 (muscle) antibody; Glycogen synthase 1 antibody; GSY antibody; GYS antibody; Gys1 antibody; GYS1_HUMAN antibody; muscle antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from Human Phospho-GYS1 (S641)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 1D1
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IF 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Gene References into Functions
  1. Insulin-stimulated glycogen synthase (GS) activity was completely ablated during hyperinsulinemic hypoglycemia, and catecholamine signaling via cAMP-dependent protein kinase (PKA) and phosphorylation of inhibiting sites on GS all increased. PMID: 28596236
  2. Overexpression of GYS1, MIF, and MYC is associated with adverse outcome and poor response to azacitidine in myelodysplastic syndromes and acute myeloid leukemia PMID: 25487600
  3. over-expression of muscle glycogen synthase (MGS)was detected in diabetic human kidney PMID: 25371328
  4. High glycogen synthase 1 expression is associated with myeloid leukemia. PMID: 25703587
  5. Data suggest that although COOH-terminal dephosphorylation is likely necessary for GS activation, protein kinase Akt-2- (Akt2)-dependent NH2-terminal dephosphorylation is site for "fine-tuning" insulin-mediated GS activation in skeletal muscle. PMID: 23321478
  6. Allosteric regulation and the relationship between phosphorylation and the kinetics of glycogen synthase. [Review] PMID: 23134486
  7. The present findings demonstrate that physical inactivity-induced insulin resistance in muscle is associated with lower content/activity of key proteins in glucose transport/phosphorylation and storage. PMID: 22403297
  8. Exercise unmaskd the effect associated with the GYS1 polymorphism, rendering carriers of this allele less susceptible to the protective effect of exercise on the risk of cardiovascular mortality. PMID: 17356695
  9. the M416V polymorphism of glycogen synthase 1 gene is not associated with insulin resistance in type 2 diabetes PMID: 12870167
  10. Phosphorylation at site 2 in the elderly and at site 3a + 3b in young twins had a genetic component. PMID: 15855312
  11. no nuclear export signal was identified in the sequence of the protein, the region comprising amino acids 555-633, which has an Arg-rich cluster involved in the allosteric activation is crucial for its nuclear concentration and aggregation. PMID: 15955076
  12. 3 siblings were identified with profound muscle glycogen deficiency and homozygous stop mutations in GYS1 PMID: 17928598
  13. Pioglitazone treatment improved insulin-stimulated glucose metabolism and glycogen synthase activity in PCOS. PMID: 18544618
  14. After overnight low muscle glycogen level and/or in response to exhausting exercise-induced glycogenolysis, GSY is associated with spherical structures at the I-band of sarcomeres. PMID: 19339242
  15. Dysregulation of glycogen synthase phosphorylation plays a major role in impaired insulin regulation of GS in obesity and type 2 diabetes mellitus. PMID: 19837931

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Involvement in disease Muscle glycogen storage disease 0 (GSD0b)
Protein Families Glycosyltransferase 3 family
Database Links

HGNC: 4706

OMIM: 138570

KEGG: hsa:2997

STRING: 9606.ENSP00000317904

UniGene: Hs.386225

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