Phospho-GYS1 (S641) Antibody

Datasheet
Code CSB-RA010078A641phHU
Size US$350
Uniprot No. P13807
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
    All lanes:Phospho-GYS1 antibody at 1.12μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 85 KDa
    Observed band size: 85 KDa

  • Immunofluorescence staining of HepG2 cells with CSB-RA010078A641phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from Human Phospho-GYS1 (S641)
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Relevance Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Glycogen [starch] synthase, muscle, GYS1, GYS
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Gene Names GYS1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Involvement in disease Muscle glycogen storage disease 0 (GSD0b)
Protein Families Glycosyltransferase 3 family
Database Links

HGNC: 4706

OMIM: 138570

KEGG: hsa:2997

STRING: 9606.ENSP00000317904

UniGene: Hs.386225

Pathway PI3K-Akt signaling pathway
AMPK signaling
Glucagon signaling pathway
Insulin signaling pathway

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