Phospho-IRF3 (S386) Antibody

Datasheet
Code CSB-RA011818A386phHU
Size US$350
Uniprot No. Q14653
Image
  • Western Blot
    Positive WB detected in:Hela whole cell lysate(treated with Calyculin A or not)
    All lanes:Phospho-IRF3 antibody at 1.03μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47 KDa
    Observed band size: 47 KDa

Immunogen A synthesized peptide derived from Human Phospho-IRF3 (S386)
Species Reactivity Human
Tested Applications ELISA, WB; Recommended dilution: WB:1:500-1:5000
Relevance Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Interferon regulatory factor 3, IRF-3, IRF3
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Research Area Immunology
Gene Names IRF3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Involvement in disease Herpes simplex encephalitis 7 (HSE7)
Subcellular Location Cytoplasm, Nucleus
Protein Families IRF family
Tissue Specificity Expressed constitutively in a variety of tissues.
Database Links

HGNC: 6118

OMIM: 603734

KEGG: hsa:3661

STRING: 9606.ENSP00000310127

UniGene: Hs.289052

Pathway NOD-like receptor signaling pathway
Toll-like receptor signaling pathway

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