Code | CSB-RA011818A386phHU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The synthesized DNA sequence corresponding to the phospho-IRF3 (S386) monoclonal antibody was cloned into the plasmid and then transfected into the cell line for expression. The product was purified through the affinity-chromatography method and obtained the p-S386-IRF3 recombinant monoclonal antibody. This anti-IRF3-pS386 recombinant antibody is a rabbit IgG and has been tested in ELISA and WB. It only targets the human IRF3 phosphorylated at Ser 386 residue.
IRF3 plays an important role in the innate immune defense against viral infection. Phosphorylation of IRF3's 7 C-terminal Ser/Thr residues, including Ser385, Ser386, Ser 396, Ser 398, Ser 402, Ser 405, and Thr 404, occurs when the host cell is infected. This phosphorylation causes IRF3 to form a complex with the coactivators CREB-binding protein (CBP)/p300, activating target genes in the nucleus. Autoinhibition is reduced when these 7 Ser/Thr residues are phosphorylated. Phosphorylation of Ser 386 was demonstrated to be essential for IRF3 activation since mutation of this residue abrogated all IRF3 activation.
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