Code | CSB-RA050476A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The phospho-MAPT (S214) recombinant monoclonal antibody generation typically starts with the insertion of the MAPT antibody-encoding gene into expression vectors. These vectors are subsequently introduced into host cells via polyethyleneimine-mediated transfection. The host cells, containing these vectors, are cultured to produce and release the antibodies. Following purification using affinity chromatography, the antibodies undergo testing through ELISA and IHC assays to confirm their recognition of the human MAPT protein phosphorylated at S214.
MAPT is a microtubule-associated protein that plays a crucial role in binding to and stabilizing microtubules, which are essential components of the neuronal cytoskeleton. Phosphorylation of MAPT at S214 can modulate the affinity of MAPT for microtubules and influence microtubule dynamics.
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